Restriction map for
pFN21AE1571
(Linear) (Six-base) MAPSORT of: KIEE1571.seq Check: 2504 from: 1 to: 780
KIEE1571.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 780
Size: 480 300
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 524 780
Size: 524 256
AlwNI CAG_nnn'CTG
Cuts at: 0 405 486 780
Size: 405 81 294
Fragments arranged by size:
405 294 81
ApaI G_GGCC'C
Cuts at: 0 624 780
Size: 624 156
ApaLI G'TGCA_C
Cuts at: 0 348 780
Size: 348 432
BanII G_rGCy'C
Cuts at: 0 53 102 412 624 780
Size: 53 49 310 212 156
Fragments arranged by size:
310 212 156 53 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 262 780
Size: 262 518
BbvCI CC'TCA_GC
Cuts at: 0 419 780
Size: 419 361
Bme1580I G_kGCm'C
Cuts at: 0 352 476 624 780
Size: 352 124 148 156
Fragments arranged by size:
352 156 148 124
BmrI ACTGGGnnnn_n'
Cuts at: 0 105 780
Size: 105 675
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 475 780
Size: 47 428 305
Fragments arranged by size:
428 305 47
Bpu10I CC'TnA_GC
Cuts at: 0 101 406 419 780
Size: 101 305 13 361
Fragments arranged by size:
361 305 101 13
BsaI GGTCTCn'nnnn_
Cuts at: 0 210 780
Size: 210 570
BsaWI w'CCGG_w
Cuts at: 0 759 780
Size: 759 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 730 733 780
Size: 730 3 47
Fragments arranged by size:
730 47 3
BseYI C'CCAG_C
Cuts at: 0 469 780
Size: 469 311
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 354 573 780
Size: 270 84 219 207
Fragments arranged by size:
270 219 207 84
BsiEI CG_ry'CG
Cuts at: 0 394 780
Size: 394 386
BsiHKAI G_wGCw'C
Cuts at: 0 352 780
Size: 352 428
BsmBI CGTCTCn'nnnn_
Cuts at: 0 401 780
Size: 401 379
Bsp1286I G_dGCh'C
Cuts at: 0 53 102 352 412 476 624 780
Size: 53 49 250 60 64 148 156
Fragments arranged by size:
250 156 148 64 60 53 49
BsrBI CCG'CTC
Cuts at: 0 388 780
Size: 388 392
BsrDI GCAATG_nn'
Cuts at: 0 327 780
Size: 327 453
BssSI C'ACGA_G
Cuts at: 0 145 780
Size: 145 635
BstXI CCAn_nnnn'nTGG
Cuts at: 0 17 780
Size: 17 763
BstYI r'GATC_y
Cuts at: 0 544 780
Size: 544 236
BtsI GCAGTG_nn'
Cuts at: 0 293 337 780
Size: 293 44 443
Fragments arranged by size:
443 293 44
DraIII CAC_nnn'GTG
Cuts at: 0 356 780
Size: 356 424
DrdI GACnn_nn'nnGTC
Cuts at: 0 390 780
Size: 390 390
EaeI y'GGCC_r
Cuts at: 0 7 532 614 780
Size: 7 525 82 166
Fragments arranged by size:
525 166 82 7
EarI CTCTTCn'nnn_
Cuts at: 0 371 780
Size: 371 409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 475 480 780
Size: 47 428 5 300
Fragments arranged by size:
428 300 47 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 148 780
Size: 148 632
EcoO109I rG'GnC_Cy
Cuts at: 0 38 402 620 621 780
Size: 38 364 218 1 159
Fragments arranged by size:
364 218 159 38 1
MscI TGG'CCA
Cuts at: 0 534 780
Size: 534 246
MslI CAynn'nnrTG
Cuts at: 0 577 780
Size: 577 203
MspA1I CmG'CkG
Cuts at: 0 469 504 704 780
Size: 469 35 200 76
Fragments arranged by size:
469 200 76 35
NspI r_CATG'y
Cuts at: 0 480 780
Size: 480 300
PasI CC'CwG_GG
Cuts at: 0 710 780
Size: 710 70
PfoI T'CCnGG_A
Cuts at: 0 740 780
Size: 740 40
PpuMI rG'GwC_Cy
Cuts at: 0 38 780
Size: 38 742
PspOMI G'GGCC_C
Cuts at: 0 620 780
Size: 620 160
PstI C_TGCA'G
Cuts at: 0 687 780
Size: 687 93
PvuII CAG'CTG
Cuts at: 0 469 504 704 780
Size: 469 35 200 76
Fragments arranged by size:
469 200 76 35
SanDI GG'GwC_CC
Cuts at: 0 38 780
Size: 38 742
SfcI C'TryA_G
Cuts at: 0 683 780
Size: 683 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 380 442 780
Size: 380 62 338
Fragments arranged by size:
380 338 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 331 363 780
Size: 331 32 417
Fragments arranged by size:
417 331 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 17 780
Size: 17 763
Enzymes that do cut:
AcuI AlfI AlwNI ApaI ApaLI BanII BbsI BbvCI
Bme1580I BmrI BpmI Bpu10I BsaI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BssSI
BstXI BstYI BtsI DraIII DrdI EaeI EarI Eco57MI
EcoNI EcoO109I MscI MslI MspA1I NspI PasI PfoI
PpuMI PspOMI PstI PvuII SanDI SfcI TaqII TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaXI BsiWI BsmI BspEI
BspHI BspMI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TatI Tth111I XbaI XhoI
XmaI XmnI ZraI