Restriction map for
pFN21AE1568
(Linear) (Six-base) MAPSORT of: KIEE1568.seq Check: 8548 from: 1 to: 927
KIEE1568.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AatII G_ACGT'C
Cuts at: 0 358 927
Size: 358 569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 209 231 445 607 739 927
Size: 116 93 22 214 162 132 188
Fragments arranged by size:
214 188 162 132 116 93 22
AlwNI CAG_nnn'CTG
Cuts at: 0 22 206 927
Size: 22 184 721
Fragments arranged by size:
721 184 22
ApoI r'AATT_y
Cuts at: 0 10 474 927
Size: 10 464 453
Fragments arranged by size:
464 453 10
AvrII C'CTAG_G
Cuts at: 0 874 927
Size: 874 53
BanI G'GyrC_C
Cuts at: 0 419 447 927
Size: 419 28 480
Fragments arranged by size:
480 419 28
BanII G_rGCy'C
Cuts at: 0 35 393 804 927
Size: 35 358 411 123
Fragments arranged by size:
411 358 123 35
BbeI G_GCGC'C
Cuts at: 0 423 927
Size: 423 504
BglI GCCn_nnn'nGGC
Cuts at: 0 417 927
Size: 417 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 927
Size: 313 614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 788 805 927
Size: 745 43 17 122
Fragments arranged by size:
745 122 43 17
BsaAI yAC'GTr
Cuts at: 0 686 927
Size: 686 241
BsaHI Gr'CG_yC
Cuts at: 0 355 420 927
Size: 355 65 507
Fragments arranged by size:
507 355 65
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 927
Size: 43 884
BseYI C'CCAG_C
Cuts at: 0 559 927
Size: 559 368
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 872 927
Size: 732 140 55
Fragments arranged by size:
732 140 55
BsiEI CG_ry'CG
Cuts at: 0 99 927
Size: 99 828
BsiHKAI G_wGCw'C
Cuts at: 0 35 393 702 927
Size: 35 358 309 225
Fragments arranged by size:
358 309 225 35
BsmI GAATG_Cn'
Cuts at: 0 787 927
Size: 787 140
Bsp1286I G_dGCh'C
Cuts at: 0 35 393 702 804 927
Size: 35 358 309 102 123
Fragments arranged by size:
358 309 123 102 35
BsrDI GCAATG_nn'
Cuts at: 0 54 606 927
Size: 54 552 321
Fragments arranged by size:
552 321 54
BsrFI r'CCGG_y
Cuts at: 0 43 927
Size: 43 884
BssHII G'CGCG_C
Cuts at: 0 101 927
Size: 101 826
BstAPI GCAn_nnn'nTGC
Cuts at: 0 206 927
Size: 206 721
BtsI GCAGTG_nn'
Cuts at: 0 743 927
Size: 743 184
EaeI y'GGCC_r
Cuts at: 0 66 96 927
Size: 66 30 831
Fragments arranged by size:
831 66 30
EagI C'GGCC_G
Cuts at: 0 96 927
Size: 96 831
EarI CTCTTCn'nnn_
Cuts at: 0 460 927
Size: 460 467
EcoICRI GAG'CTC
Cuts at: 0 33 391 927
Size: 33 358 536
Fragments arranged by size:
536 358 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 209 231 313 445 607 739 927
Size: 116 93 22 82 132 162 132 188
Fragments arranged by size:
188 162 132 132 116 93 82 22
EcoRI G'AATT_C
Cuts at: 0 474 927
Size: 474 453
HaeII r_GCGC'y
Cuts at: 0 423 927
Size: 423 504
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 194 226 375 407 817 849 927
Size: 194 32 149 32 410 32 78
Fragments arranged by size:
410 194 149 78 32 32 32
HincII GTy'rAC
Cuts at: 0 318 927
Size: 318 609
KasI G'GCGC_C
Cuts at: 0 419 927
Size: 419 508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 927
Size: 188 739
NaeI GCC'GGC
Cuts at: 0 45 927
Size: 45 882
NarI GG'CG_CC
Cuts at: 0 420 927
Size: 420 507
NgoMIV G'CCGG_C
Cuts at: 0 43 927
Size: 43 884
NspI r_CATG'y
Cuts at: 0 179 927
Size: 179 748
PstI C_TGCA'G
Cuts at: 0 119 202 338 927
Size: 119 83 136 589
Fragments arranged by size:
589 136 119 83
SacI G_AGCT'C
Cuts at: 0 35 393 927
Size: 35 358 534
Fragments arranged by size:
534 358 35
SfcI C'TryA_G
Cuts at: 0 115 198 334 792 862 927
Size: 115 83 136 458 70 65
Fragments arranged by size:
458 136 115 83 70 65
SfoI GGC'GCC
Cuts at: 0 421 927
Size: 421 506
SmlI C'TyrA_G
Cuts at: 0 760 803 820 927
Size: 760 43 17 107
Fragments arranged by size:
760 107 43 17
StuI AGG'CCT
Cuts at: 0 82 927
Size: 82 845
StyI C'CwwG_G
Cuts at: 0 236 443 874 927
Size: 236 207 431 53
Fragments arranged by size:
431 236 207 53
TatI w'GTAC_w
Cuts at: 0 644 725 927
Size: 644 81 202
Fragments arranged by size:
644 202 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 764 796 927
Size: 764 32 131
Fragments arranged by size:
764 131 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 393 927
Size: 393 534
XmnI GAAnn'nnTTC
Cuts at: 0 152 787 927
Size: 152 635 140
Fragments arranged by size:
635 152 140
ZraI GAC'GTC
Cuts at: 0 356 927
Size: 356 571
Enzymes that do cut:
AatII AcuI AlwNI ApoI AvrII BanI BanII BbeI
BglI BpmI BpuEI BsaAI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BsrDI BsrFI BssHII BstAPI
BtsI EaeI EagI EarI EcoICRI Eco57MI EcoRI HaeII
Hin4I HincII KasI MmeI NaeI NarI NgoMIV NspI
PstI SacI SfcI SfoI SmlI StuI StyI TatI
TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaBI BsaWI BsaXI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrGI BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EciI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI