Restriction map for
pFN21AE1563
(Linear) (Six-base) MAPSORT of: KIEE1563.seq Check: 9656 from: 1 to: 1374
KIEE1563.orf
With 182 enzymes: SgfI *
July 3, 2012 16:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 554 1374
Size: 554 820
AccI GT'mk_AC
Cuts at: 0 143 770 1374
Size: 143 627 604
Fragments arranged by size:
627 604 143
AclI AA'CG_TT
Cuts at: 0 1225 1374
Size: 1225 149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1091 1374
Size: 1091 283
AhdI GACnn_n'nnGTC
Cuts at: 0 1208 1374
Size: 1208 166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 889 1374
Size: 889 485
AlwNI CAG_nnn'CTG
Cuts at: 0 759 1002 1174 1198 1374
Size: 759 243 172 24 176
Fragments arranged by size:
759 243 176 172 24
ApaI G_GGCC'C
Cuts at: 0 424 1374
Size: 424 950
AvaI C'yCGr_G
Cuts at: 0 583 590 1374
Size: 583 7 784
Fragments arranged by size:
784 583 7
BanII G_rGCy'C
Cuts at: 0 221 424 1284 1374
Size: 221 203 860 90
Fragments arranged by size:
860 221 203 90
BbsI GAAGACnn'nnnn_
Cuts at: 0 12 1374
Size: 12 1362
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 391 425 1374
Size: 391 34 949
Fragments arranged by size:
949 391 34
Bme1580I G_kGCm'C
Cuts at: 0 424 1374
Size: 424 950
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1298 1374
Size: 1298 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1374
Size: 208 1166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1244 1374
Size: 1244 130
BsaI GGTCTCn'nnnn_
Cuts at: 0 580 1374
Size: 580 794
BsaAI yAC'GTr
Cuts at: 0 183 1374
Size: 183 1191
BsaWI w'CCGG_w
Cuts at: 0 320 602 1374
Size: 320 282 772
Fragments arranged by size:
772 320 282
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 292 322 641 671 1374
Size: 292 30 319 30 703
Fragments arranged by size:
703 319 292 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 328 967 1290 1374
Size: 22 306 639 323 84
Fragments arranged by size:
639 323 306 84 22
BseYI C'CCAG_C
Cuts at: 0 972 1285 1374
Size: 972 313 89
Fragments arranged by size:
972 313 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1336 1374
Size: 1336 38
BsiHKAI G_wGCw'C
Cuts at: 0 695 1284 1374
Size: 695 589 90
Fragments arranged by size:
695 589 90
BsmI GAATG_Cn'
Cuts at: 0 127 365 878 1374
Size: 127 238 513 496
Fragments arranged by size:
513 496 238 127
Bsp1286I G_dGCh'C
Cuts at: 0 221 424 695 1284 1374
Size: 221 203 271 589 90
Fragments arranged by size:
589 271 221 203 90
BspEI T'CCGG_A
Cuts at: 0 320 1374
Size: 320 1054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 17 554 1374
Size: 17 537 820
Fragments arranged by size:
820 537 17
BsrGI T'GTAC_A
Cuts at: 0 104 1374
Size: 104 1270
BssSI C'ACGA_G
Cuts at: 0 214 1374
Size: 214 1160
BstEII G'GTnAC_C
Cuts at: 0 60 1111 1374
Size: 60 1051 263
Fragments arranged by size:
1051 263 60
BstXI CCAn_nnnn'nTGG
Cuts at: 0 563 1374
Size: 563 811
BtgI C'CryG_G
Cuts at: 0 645 1116 1205 1374
Size: 645 471 89 169
Fragments arranged by size:
645 471 169 89
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 449 484 1374
Size: 449 35 890
Fragments arranged by size:
890 449 35
EaeI y'GGCC_r
Cuts at: 0 662 761 1374
Size: 662 99 613
Fragments arranged by size:
662 613 99
EarI CTCTTCn'nnn_
Cuts at: 0 46 754 944 1374
Size: 46 708 190 430
Fragments arranged by size:
708 430 190 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 700 1374
Size: 700 674
EcoICRI GAG'CTC
Cuts at: 0 1282 1374
Size: 1282 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1091 1374
Size: 208 883 283
Fragments arranged by size:
883 283 208
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1006 1374
Size: 1006 368
EcoO109I rG'GnC_Cy
Cuts at: 0 420 532 1210 1366 1374
Size: 420 112 678 156 8
Fragments arranged by size:
678 420 156 112 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 1194 1226 1374
Size: 30 1164 32 148
Fragments arranged by size:
1164 148 32 30
HincII GTy'rAC
Cuts at: 0 1233 1374
Size: 1233 141
HpaI GTT'AAC
Cuts at: 0 1233 1374
Size: 1233 141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 784 1374
Size: 278 506 590
Fragments arranged by size:
590 506 278
MscI TGG'CCA
Cuts at: 0 664 763 1374
Size: 664 99 611
Fragments arranged by size:
664 611 99
MslI CAynn'nnrTG
Cuts at: 0 80 786 1374
Size: 80 706 588
Fragments arranged by size:
706 588 80
MspA1I CmG'CkG
Cuts at: 0 294 963 1356 1374
Size: 294 669 393 18
Fragments arranged by size:
669 393 294 18
NcoI C'CATG_G
Cuts at: 0 1116 1374
Size: 1116 258
NspI r_CATG'y
Cuts at: 0 255 1374
Size: 255 1119
PasI CC'CwG_GG
Cuts at: 0 417 1374
Size: 417 957
PflMI CCAn_nnn'nTGG
Cuts at: 0 759 1002 1374
Size: 759 243 372
Fragments arranged by size:
759 372 243
PfoI T'CCnGG_A
Cuts at: 0 353 1001 1374
Size: 353 648 373
Fragments arranged by size:
648 373 353
PpuMI rG'GwC_Cy
Cuts at: 0 1210 1374
Size: 1210 164
PspOMI G'GGCC_C
Cuts at: 0 420 1374
Size: 420 954
PstI C_TGCA'G
Cuts at: 0 293 1361 1374
Size: 293 1068 13
Fragments arranged by size:
1068 293 13
PvuII CAG'CTG
Cuts at: 0 294 1356 1374
Size: 294 1062 18
Fragments arranged by size:
1062 294 18
SacI G_AGCT'C
Cuts at: 0 1284 1374
Size: 1284 90
SapI GCTCTTCn'nnn_
Cuts at: 0 754 1374
Size: 754 620
SfcI C'TryA_G
Cuts at: 0 144 289 1357 1374
Size: 144 145 1068 17
Fragments arranged by size:
1068 145 144 17
SmaI CCC'GGG
Cuts at: 0 592 1374
Size: 592 782
SmlI C'TyrA_G
Cuts at: 0 1259 1374
Size: 1259 115
StyI C'CwwG_G
Cuts at: 0 221 764 798 1116 1374
Size: 221 543 34 318 258
Fragments arranged by size:
543 318 258 221 34
TatI w'GTAC_w
Cuts at: 0 104 1374
Size: 104 1270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 292 324 641 673 1374
Size: 292 32 317 32 701
Fragments arranged by size:
701 317 292 32 32
XmaI C'CCGG_G
Cuts at: 0 590 1374
Size: 590 784
XmnI GAAnn'nnTTC
Cuts at: 0 1010 1374
Size: 1010 364
Enzymes that do cut:
AarI AccI AclI AcuI AhdI AlfI AlwNI ApaI
AvaI BanII BbsI BcgI Bme1580I BplI BpmI BpuEI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrGI BssSI BstEII BstXI
BtgI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I Hin4I HincII HpaI MmeI MscI MslI MspA1I
NcoI NspI PasI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SapI SfcI SmaI SmlI StyI TatI
TstI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AgeI AleI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI Bpu10I BsaBI BsaHI BsiEI
BsiWI BsmBI BspHI BsrBI BsrDI BsrFI BssHII BstAPI
BstBI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI DraI
DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
ZraI