(Linear) (Six-base) MAPSORT of: KIEE1561.seq  Check: 6581  from: 1  to: 294



KIEE1561.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:42  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     294 
   Size:         98     196
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     230     262     294 
   Size:        230      32      32

  Fragments arranged by size: 

                 230      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     255     294 
   Size:        255      39
 
ApaLI G'TGCA_C

Cuts at:      0     212     294 
   Size:        212      82
 
BanI G'GyrC_C

Cuts at:      0      64     168     294 
   Size:         64     104     126

  Fragments arranged by size: 

                 126     104      64
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      86     294 
   Size:         86     208
 
BbvCI CC'TCA_GC

Cuts at:      0      19     267     294 
   Size:         19     248      27

  Fragments arranged by size: 

                 248      27      19
 
Bme1580I G_kGCm'C

Cuts at:      0     216     294 
   Size:        216      78
 
Bpu10I CC'TnA_GC

Cuts at:      0      19     267     294 
   Size:         19     248      27

  Fragments arranged by size: 

                 248      27      19
 
BseYI C'CCAG_C

Cuts at:      0      45     294 
   Size:         45     249
 
BsiHKAI G_wGCw'C

Cuts at:      0     216     294 
   Size:        216      78
 
Bsp1286I G_dGCh'C

Cuts at:      0     216     294 
   Size:        216      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     294 
   Size:         98     196
 
EcoO109I rG'GnC_Cy

Cuts at:      0     263     294 
   Size:        263      31
 
HaeII r_GCGC'y

Cuts at:      0     285     294 
   Size:        285       9
 
PasI CC'CwG_GG

Cuts at:      0     115     230     294 
   Size:        115     115      64

  Fragments arranged by size: 

                 115     115      64
 
PpuMI rG'GwC_Cy

Cuts at:      0     263     294 
   Size:        263      31
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     124     156     294 
   Size:        124      32     138

  Fragments arranged by size: 

                 138     124      32
 
PstI C_TGCA'G

Cuts at:      0     166     260     294 
   Size:        166      94      34

  Fragments arranged by size: 

                 166      94      34
 
SfcI C'TryA_G

Cuts at:      0     162     176     256     294 
   Size:        162      14      80      38

  Fragments arranged by size: 

                 162      80      38      14
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     227     294 
   Size:        227      67
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI    ApaLI     BanI     BbsI    BbvCI Bme1580I
   Bpu10I    BseYI  BsiHKAI Bsp1286I  Eco57MI EcoO109I    HaeII     PasI
    PpuMI     PsrI     PstI     SfcI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI