Restriction map for
pFN21AE1560
(Linear) (Six-base) MAPSORT of: KIEE1560.seq Check: 6971 from: 1 to: 738
KIEE1560.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AccI GT'mk_AC
Cuts at: 0 683 738
Size: 683 55
AgeI A'CCGG_T
Cuts at: 0 586 738
Size: 586 152
AleI CACnn'nnGTG
Cuts at: 0 367 738
Size: 367 371
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 509 541 738
Size: 509 32 197
Fragments arranged by size:
509 197 32
AlwNI CAG_nnn'CTG
Cuts at: 0 120 738
Size: 120 618
ApaI G_GGCC'C
Cuts at: 0 228 614 738
Size: 228 386 124
Fragments arranged by size:
386 228 124
BamHI G'GATC_C
Cuts at: 0 567 738
Size: 567 171
BanII G_rGCy'C
Cuts at: 0 228 614 738
Size: 228 386 124
Fragments arranged by size:
386 228 124
BbsI GAAGACnn'nnnn_
Cuts at: 0 583 738
Size: 583 155
BglI GCCn_nnn'nGGC
Cuts at: 0 381 738
Size: 381 357
Bme1580I G_kGCm'C
Cuts at: 0 228 614 738
Size: 228 386 124
Fragments arranged by size:
386 228 124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 659 738
Size: 361 298 79
Fragments arranged by size:
361 298 79
BsaI GGTCTCn'nnnn_
Cuts at: 0 699 738
Size: 699 39
BsaWI w'CCGG_w
Cuts at: 0 586 738
Size: 586 152
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 4 70 738
Size: 4 66 668
Fragments arranged by size:
668 66 4
BseYI C'CCAG_C
Cuts at: 0 46 254 437 738
Size: 46 208 183 301
Fragments arranged by size:
301 208 183 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 517 738
Size: 444 73 221
Fragments arranged by size:
444 221 73
BsiHKAI G_wGCw'C
Cuts at: 0 460 738
Size: 460 278
BsmBI CGTCTCn'nnnn_
Cuts at: 0 526 738
Size: 526 212
Bsp1286I G_dGCh'C
Cuts at: 0 228 460 614 738
Size: 228 232 154 124
Fragments arranged by size:
232 228 154 124
BsrBI CCG'CTC
Cuts at: 0 249 738
Size: 249 489
BsrFI r'CCGG_y
Cuts at: 0 586 738
Size: 586 152
BssSI C'ACGA_G
Cuts at: 0 616 738
Size: 616 122
BstYI r'GATC_y
Cuts at: 0 567 738
Size: 567 171
BtgI C'CryG_G
Cuts at: 0 528 738
Size: 528 210
BtsI GCAGTG_nn'
Cuts at: 0 189 432 472 738
Size: 189 243 40 266
Fragments arranged by size:
266 243 189 40
EarI CTCTTCn'nnn_
Cuts at: 0 124 133 529 738
Size: 124 9 396 209
Fragments arranged by size:
396 209 124 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 659 738
Size: 361 298 79
Fragments arranged by size:
361 298 79
EcoO109I rG'GnC_Cy
Cuts at: 0 224 610 611 738
Size: 224 386 1 127
Fragments arranged by size:
386 224 127 1
HaeII r_GCGC'y
Cuts at: 0 735 738
Size: 735 3
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 171 203 671 703 738
Size: 171 32 468 32 35
Fragments arranged by size:
468 171 35 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 341 738
Size: 115 226 397
Fragments arranged by size:
397 226 115
MslI CAynn'nnrTG
Cuts at: 0 157 367 738
Size: 157 210 371
Fragments arranged by size:
371 210 157
MspA1I CmG'CkG
Cuts at: 0 184 339 367 420 738
Size: 184 155 28 53 318
Fragments arranged by size:
318 184 155 53 28
NcoI C'CATG_G
Cuts at: 0 528 738
Size: 528 210
PasI CC'CwG_GG
Cuts at: 0 40 738
Size: 40 698
PfoI T'CCnGG_A
Cuts at: 0 675 738
Size: 675 63
PspOMI G'GGCC_C
Cuts at: 0 224 610 738
Size: 224 386 128
Fragments arranged by size:
386 224 128
PstI C_TGCA'G
Cuts at: 0 338 479 738
Size: 338 141 259
Fragments arranged by size:
338 259 141
PvuII CAG'CTG
Cuts at: 0 184 339 367 420 738
Size: 184 155 28 53 318
Fragments arranged by size:
318 184 155 53 28
SfcI C'TryA_G
Cuts at: 0 334 475 738
Size: 334 141 263
Fragments arranged by size:
334 263 141
StyI C'CwwG_G
Cuts at: 0 218 528 636 738
Size: 218 310 108 102
Fragments arranged by size:
310 218 108 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 129 161 496 528 738
Size: 129 32 335 32 210
Fragments arranged by size:
335 210 129 32 32
Enzymes that do cut:
AccI AgeI AleI AloI AlwNI ApaI BamHI BanII
BbsI BglI Bme1580I BpmI BsaI BsaWI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BssSI BstYI
BtgI BtsI EarI Eco57MI EcoO109I HaeII Hin4I MmeI
MslI MspA1I NcoI PasI PfoI PspOMI PstI PvuII
SfcI StyI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI