(Linear) (Six-base) MAPSORT of: KIEE1555.seq  Check: 4491  from: 1  to: 459



KIEE1555.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:42  ..

AccI GT'mk_AC

Cuts at:      0     152     459 
   Size:        152     307
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     426     459 
   Size:        426      33
 
AfeI AGC'GCT

Cuts at:      0     315     459 
   Size:        315     144
 
ApoI r'AATT_y

Cuts at:      0     371     459 
   Size:        371      88
 
AseI AT'TA_AT

Cuts at:      0     383     459 
   Size:        383      76
 
AvaI C'yCGr_G

Cuts at:      0      51      97     459 
   Size:         51      46     362

  Fragments arranged by size: 

                 362      51      46
 
Bme1580I G_kGCm'C

Cuts at:      0      50     459 
   Size:         50     409
 
BmgBI CAC'GTC

Cuts at:      0      45     459 
   Size:         45     414
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282     459 
   Size:        282     177
 
BsaBI GATnn'nnATC

Cuts at:      0     255     459 
   Size:        255     204
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     459 
   Size:         50     409
 
BsrGI T'GTAC_A

Cuts at:      0     305     459 
   Size:        305     154
 
BstZ17I GTA'TAC

Cuts at:      0     153     459 
   Size:        153     306
 
BtgI C'CryG_G

Cuts at:      0     128     285     459 
   Size:        128     157     174

  Fragments arranged by size: 

                 174     157     128
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     396     459 
   Size:        396      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282     426     459 
   Size:        282     144      33

  Fragments arranged by size: 

                 282     144      33
 
HaeII r_GCGC'y

Cuts at:      0     317     459 
   Size:        317     142
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      34      66     459 
   Size:         34      32     393

  Fragments arranged by size: 

                 393      34      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      65     459 
   Size:         65     394
 
NcoI C'CATG_G

Cuts at:      0     128     459 
   Size:        128     331
 
NspI r_CATG'y

Cuts at:      0     337     459 
   Size:        337     122
 
PfoI T'CCnGG_A

Cuts at:      0     259     459 
   Size:        259     200
 
StyI C'CwwG_G

Cuts at:      0     128     459 
   Size:        128     331
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     157     162     459 
   Size:        157       5     297

  Fragments arranged by size: 

                 297     157       5
 
TatI w'GTAC_w

Cuts at:      0     305     330     459 
   Size:        305      25     129

  Fragments arranged by size: 

                 305     129      25
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     292     459 
   Size:        292     167
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     ApoI     AseI     AvaI Bme1580I    BmgBI
     BpmI    BsaBI Bsp1286I    BsrGI  BstZ17I     BtgI    BtgZI  Eco57MI
    HaeII    Hin4I     MmeI     NcoI     NspI     PfoI     StyI    TaqII
     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI