Restriction map for
pFN21AE1553
(Linear) (Six-base) MAPSORT of: KIEE1553.seq Check: 3505 from: 1 to: 1896
KIEE1553.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1896
Size: 503 1393
AflIII A'CryG_T
Cuts at: 0 263 1074 1896
Size: 263 811 822
Fragments arranged by size:
822 811 263
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 776 808 1896
Size: 776 32 1088
Fragments arranged by size:
1088 776 32
AlwNI CAG_nnn'CTG
Cuts at: 0 762 1565 1896
Size: 762 803 331
Fragments arranged by size:
803 762 331
ApoI r'AATT_y
Cuts at: 0 459 1896
Size: 459 1437
AvaI C'yCGr_G
Cuts at: 0 836 877 1896
Size: 836 41 1019
Fragments arranged by size:
1019 836 41
BanI G'GyrC_C
Cuts at: 0 100 1896
Size: 100 1796
BanII G_rGCy'C
Cuts at: 0 1117 1337 1502 1896
Size: 1117 220 165 394
Fragments arranged by size:
1117 394 220 165
BbsI GAAGACnn'nnnn_
Cuts at: 0 1514 1896
Size: 1514 382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1257 1291 1896
Size: 1257 34 605
Fragments arranged by size:
1257 605 34
Bme1580I G_kGCm'C
Cuts at: 0 336 1896
Size: 336 1560
BmgBI CAC'GTC
Cuts at: 0 1075 1896
Size: 1075 821
BmrI ACTGGGnnnn_n'
Cuts at: 0 295 551 1553 1896
Size: 295 256 1002 343
Fragments arranged by size:
1002 343 295 256
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1205 1896
Size: 1205 691
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 1896
Size: 1000 896
BsaAI yAC'GTr
Cuts at: 0 264 1896
Size: 264 1632
BsaBI GATnn'nnATC
Cuts at: 0 703 1896
Size: 703 1193
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 895 997 1194 1355 1896
Size: 895 102 197 161 541
Fragments arranged by size:
895 541 197 161 102
BseYI C'CCAG_C
Cuts at: 0 1521 1896
Size: 1521 375
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 446 1896
Size: 55 391 1450
Fragments arranged by size:
1450 391 55
BsiEI CG_ry'CG
Cuts at: 0 660 1896
Size: 660 1236
BsmBI CGTCTCn'nnnn_
Cuts at: 0 647 742 1896
Size: 647 95 1154
Fragments arranged by size:
1154 647 95
Bsp1286I G_dGCh'C
Cuts at: 0 336 1117 1337 1502 1896
Size: 336 781 220 165 394
Fragments arranged by size:
781 394 336 220 165
BspHI T'CATG_A
Cuts at: 0 1221 1896
Size: 1221 675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 77 1896
Size: 77 1819
BsrBI CCG'CTC
Cuts at: 0 419 1896
Size: 419 1477
BsrDI GCAATG_nn'
Cuts at: 0 717 1155 1896
Size: 717 438 741
Fragments arranged by size:
741 717 438
BsrFI r'CCGG_y
Cuts at: 0 659 1135 1896
Size: 659 476 761
Fragments arranged by size:
761 659 476
BstBI TT'CG_AA
Cuts at: 0 405 1896
Size: 405 1491
BstYI r'GATC_y
Cuts at: 0 349 552 1896
Size: 349 203 1344
Fragments arranged by size:
1344 349 203
BtgI C'CryG_G
Cuts at: 0 182 1600 1896
Size: 182 1418 296
Fragments arranged by size:
1418 296 182
BtsI GCAGTG_nn'
Cuts at: 0 473 1439 1896
Size: 473 966 457
Fragments arranged by size:
966 473 457
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1589 1624 1896
Size: 1589 35 272
Fragments arranged by size:
1589 272 35
DraI TTT'AAA
Cuts at: 0 375 1896
Size: 375 1521
DraIII CAC_nnn'GTG
Cuts at: 0 1561 1896
Size: 1561 335
EaeI y'GGCC_r
Cuts at: 0 312 657 1896
Size: 312 345 1239
Fragments arranged by size:
1239 345 312
EagI C'GGCC_G
Cuts at: 0 657 1896
Size: 657 1239
EarI CTCTTCn'nnn_
Cuts at: 0 127 1896
Size: 127 1769
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1896
Size: 503 1393
EcoO109I rG'GnC_Cy
Cuts at: 0 816 1896
Size: 816 1080
FspI TGC'GCA
Cuts at: 0 907 1896
Size: 907 989
HaeII r_GCGC'y
Cuts at: 0 10 141 1896
Size: 10 131 1755
Fragments arranged by size:
1755 131 10
HincII GTy'rAC
Cuts at: 0 693 1896
Size: 693 1203
HindIII A'AGCT_T
Cuts at: 0 1475 1896
Size: 1475 421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 645 1562 1896
Size: 645 917 334
Fragments arranged by size:
917 645 334
MspA1I CmG'CkG
Cuts at: 0 211 1110 1157 1648 1896
Size: 211 899 47 491 248
Fragments arranged by size:
899 491 248 211 47
NaeI GCC'GGC
Cuts at: 0 661 1137 1896
Size: 661 476 759
Fragments arranged by size:
759 661 476
NgoMIV G'CCGG_C
Cuts at: 0 659 1135 1896
Size: 659 476 761
Fragments arranged by size:
761 659 476
NspI r_CATG'y
Cuts at: 0 1819 1896
Size: 1819 77
PasI CC'CwG_GG
Cuts at: 0 1165 1896
Size: 1165 731
PflMI CCAn_nnn'nTGG
Cuts at: 0 1548 1565 1896
Size: 1548 17 331
Fragments arranged by size:
1548 331 17
PshAI GACnn'nnGTC
Cuts at: 0 1414 1896
Size: 1414 482
PstI C_TGCA'G
Cuts at: 0 210 1516 1896
Size: 210 1306 380
Fragments arranged by size:
1306 380 210
PvuII CAG'CTG
Cuts at: 0 211 1157 1648 1896
Size: 211 946 491 248
Fragments arranged by size:
946 491 248 211
ScaI AGT'ACT
Cuts at: 0 1469 1896
Size: 1469 427
SfcI C'TryA_G
Cuts at: 0 206 828 1512 1896
Size: 206 622 684 384
Fragments arranged by size:
684 622 384 206
SmlI C'TyrA_G
Cuts at: 0 979 1896
Size: 979 917
StyI C'CwwG_G
Cuts at: 0 28 493 1896
Size: 28 465 1403
Fragments arranged by size:
1403 465 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1572 1896
Size: 1572 324
TatI w'GTAC_w
Cuts at: 0 1467 1798 1896
Size: 1467 331 98
Fragments arranged by size:
1467 331 98
Tth111I GACn'n_nGTC
Cuts at: 0 971 1896
Size: 971 925
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1545 1562 1896
Size: 1545 17 334
Fragments arranged by size:
1545 334 17
XmnI GAAnn'nnTTC
Cuts at: 0 488 1896
Size: 488 1408
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApoI AvaI BanI BanII
BbsI BcgI Bme1580I BmgBI BmrI BplI BpuEI BsaAI
BsaBI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BstBI BstYI BtgI BtsI
CspCI DraI DraIII EaeI EagI EarI Eco57MI EcoO109I
FspI HaeII HincII HindIII MmeI MspA1I NaeI NgoMIV
NspI PasI PflMI PshAI PstI PvuII ScaI SfcI
SmlI StyI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI BmtI BpmI Bpu10I BsaI BsaHI
BsaWI BsaXI BsiHKAI BsiWI BsmI BspEI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspAI Hin4I HpaI KasI KpnI MfeI MluI MscI
MslI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI XbaI XhoI XmaI ZraI