Restriction map for
pFN21AE1549
(Linear) (Six-base) MAPSORT of: KIEE1549.seq Check: 7319 from: 1 to: 1527
KIEE1549.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AccI GT'mk_AC
Cuts at: 0 140 407 1195 1527
Size: 140 267 788 332
Fragments arranged by size:
788 332 267 140
ApoI r'AATT_y
Cuts at: 0 265 1098 1374 1429 1527
Size: 265 833 276 55 98
Fragments arranged by size:
833 276 265 98 55
BanII G_rGCy'C
Cuts at: 0 620 786 1527
Size: 620 166 741
Fragments arranged by size:
741 620 166
BbsI GAAGACnn'nnnn_
Cuts at: 0 392 1527
Size: 392 1135
BbvCI CC'TCA_GC
Cuts at: 0 130 1527
Size: 130 1397
BciVI GTATCCnnnnn_n'
Cuts at: 0 1441 1527
Size: 1441 86
BclI T'GATC_A
Cuts at: 0 1151 1527
Size: 1151 376
BfrBI ATG'CAT
Cuts at: 0 280 1527
Size: 280 1247
BmrI ACTGGGnnnn_n'
Cuts at: 0 1492 1527
Size: 1492 35
BmtI G_CTAG'C
Cuts at: 0 638 1527
Size: 638 889
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1190 1527
Size: 1190 337
Bpu10I CC'TnA_GC
Cuts at: 0 130 1527
Size: 130 1397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 1072 1527
Size: 824 248 455
Fragments arranged by size:
824 455 248
BsaHI Gr'CG_yC
Cuts at: 0 897 1353 1527
Size: 897 456 174
Fragments arranged by size:
897 456 174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 196 226 1188 1218 1527
Size: 196 30 962 30 309
Fragments arranged by size:
962 309 196 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1166 1527
Size: 1166 361
BseYI C'CCAG_C
Cuts at: 0 13 27 57 1308 1348 1527
Size: 13 14 30 1251 40 179
Fragments arranged by size:
1251 179 40 30 14 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 1527
Size: 344 1183
BsiHKAI G_wGCw'C
Cuts at: 0 620 786 1527
Size: 620 166 741
Fragments arranged by size:
741 620 166
BsmI GAATG_Cn'
Cuts at: 0 399 1527
Size: 399 1128
Bsp1286I G_dGCh'C
Cuts at: 0 620 786 1527
Size: 620 166 741
Fragments arranged by size:
741 620 166
BspHI T'CATG_A
Cuts at: 0 1154 1527
Size: 1154 373
BsrFI r'CCGG_y
Cuts at: 0 160 1527
Size: 160 1367
BstYI r'GATC_y
Cuts at: 0 1092 1527
Size: 1092 435
BstZ17I GTA'TAC
Cuts at: 0 1196 1527
Size: 1196 331
BtgI C'CryG_G
Cuts at: 0 314 1527
Size: 314 1213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 82 1527
Size: 82 1445
BtsI GCAGTG_nn'
Cuts at: 0 361 1527
Size: 361 1166
DraI TTT'AAA
Cuts at: 0 522 1527
Size: 522 1005
DrdI GACnn_nn'nnGTC
Cuts at: 0 1404 1527
Size: 1404 123
EaeI y'GGCC_r
Cuts at: 0 155 162 998 1166 1527
Size: 155 7 836 168 361
Fragments arranged by size:
836 361 168 155 7
EcoICRI GAG'CTC
Cuts at: 0 618 784 1527
Size: 618 166 743
Fragments arranged by size:
743 618 166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1190 1527
Size: 1190 337
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 579 1527
Size: 579 948
HincII GTy'rAC
Cuts at: 0 670 1527
Size: 670 857
MfeI C'AATT_G
Cuts at: 0 260 1527
Size: 260 1267
MscI TGG'CCA
Cuts at: 0 1000 1168 1527
Size: 1000 168 359
Fragments arranged by size:
1000 359 168
MspA1I CmG'CkG
Cuts at: 0 177 693 1527
Size: 177 516 834
Fragments arranged by size:
834 516 177
NaeI GCC'GGC
Cuts at: 0 162 1527
Size: 162 1365
NcoI C'CATG_G
Cuts at: 0 314 1527
Size: 314 1213
NdeI CA'TA_TG
Cuts at: 0 282 1527
Size: 282 1245
NgoMIV G'CCGG_C
Cuts at: 0 160 1527
Size: 160 1367
NheI G'CTAG_C
Cuts at: 0 634 1527
Size: 634 893
NsiI A_TGCA'T
Cuts at: 0 282 1527
Size: 282 1245
NspI r_CATG'y
Cuts at: 0 238 532 1527
Size: 238 294 995
Fragments arranged by size:
995 294 238
PflMI CCAn_nnn'nTGG
Cuts at: 0 69 229 1527
Size: 69 160 1298
Fragments arranged by size:
1298 160 69
PfoI T'CCnGG_A
Cuts at: 0 1206 1527
Size: 1206 321
PstI C_TGCA'G
Cuts at: 0 41 1527
Size: 41 1486
PvuII CAG'CTG
Cuts at: 0 177 693 1527
Size: 177 516 834
Fragments arranged by size:
834 516 177
SacI G_AGCT'C
Cuts at: 0 620 786 1527
Size: 620 166 741
Fragments arranged by size:
741 620 166
SexAI A'CCwGG_T
Cuts at: 0 167 741 1527
Size: 167 574 786
Fragments arranged by size:
786 574 167
SfcI C'TryA_G
Cuts at: 0 37 408 1527
Size: 37 371 1119
Fragments arranged by size:
1119 371 37
SmlI C'TyrA_G
Cuts at: 0 839 1087 1527
Size: 839 248 440
Fragments arranged by size:
839 440 248
SpeI A'CTAG_T
Cuts at: 0 973 1527
Size: 973 554
SphI G_CATG'C
Cuts at: 0 238 1527
Size: 238 1289
StyI C'CwwG_G
Cuts at: 0 228 314 1527
Size: 228 86 1213
Fragments arranged by size:
1213 228 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1202 1234 1527
Size: 1202 32 293
Fragments arranged by size:
1202 293 32
XbaI T'CTAG_A
Cuts at: 0 270 1527
Size: 270 1257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 646 1527
Size: 646 881
Enzymes that do cut:
AccI ApoI BanII BbsI BbvCI BciVI BclI BfrBI
BmrI BmtI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrFI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EcoICRI
Eco57MI FalI HincII MfeI MscI MspA1I NaeI NcoI
NdeI NgoMIV NheI NsiI NspI PflMI PfoI PstI
PvuII SacI SexAI SfcI SmlI SpeI SphI StyI
TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BcgI BglI BglII BlpI Bme1580I BmgBI BplI
BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI Bsu36I ClaI CspCI DraIII EagI EarI
EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HindIII HpaI KasI KpnI MluI MmeI
MslI NarI NotI NruI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI
StuI SwaI TaqII TatI Tth111I XhoI XmaI XmnI
ZraI