Restriction map for
pFN21AE1548
(Linear) (Six-base) MAPSORT of: KIEE1548.seq Check: 6564 from: 1 to: 783
KIEE1548.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 619 783
Size: 619 164
AatII G_ACGT'C
Cuts at: 0 605 783
Size: 605 178
AccI GT'mk_AC
Cuts at: 0 605 783
Size: 605 178
AleI CACnn'nnGTG
Cuts at: 0 82 783
Size: 82 701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 382 783
Size: 382 401
AlwNI CAG_nnn'CTG
Cuts at: 0 567 783
Size: 567 216
AvaI C'yCGr_G
Cuts at: 0 106 308 733 783
Size: 106 202 425 50
Fragments arranged by size:
425 202 106 50
BamHI G'GATC_C
Cuts at: 0 23 783
Size: 23 760
BanII G_rGCy'C
Cuts at: 0 356 386 666 776 783
Size: 356 30 280 110 7
Fragments arranged by size:
356 280 110 30 7
BbvCI CC'TCA_GC
Cuts at: 0 89 783
Size: 89 694
BglI GCCn_nnn'nGGC
Cuts at: 0 255 783
Size: 255 528
BlpI GC'TnA_GC
Cuts at: 0 73 783
Size: 73 710
BmgBI CAC'GTC
Cuts at: 0 225 783
Size: 225 558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 319 619 783
Size: 50 269 300 164
Fragments arranged by size:
300 269 164 50
Bpu10I CC'TnA_GC
Cuts at: 0 89 783
Size: 89 694
BsaAI yAC'GTr
Cuts at: 0 618 783
Size: 618 165
BsaBI GATnn'nnATC
Cuts at: 0 461 783
Size: 461 322
BsaHI Gr'CG_yC
Cuts at: 0 247 602 783
Size: 247 355 181
Fragments arranged by size:
355 247 181
BsaWI w'CCGG_w
Cuts at: 0 482 783
Size: 482 301
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 698 728 783
Size: 698 30 55
Fragments arranged by size:
698 55 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 520 783
Size: 214 306 263
Fragments arranged by size:
306 263 214
BseYI C'CCAG_C
Cuts at: 0 252 590 628 717 738 783
Size: 252 338 38 89 21 45
Fragments arranged by size:
338 252 89 45 38 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 763 783
Size: 351 412 20
Fragments arranged by size:
412 351 20
BsiHKAI G_wGCw'C
Cuts at: 0 79 356 626 783
Size: 79 277 270 157
Fragments arranged by size:
277 270 157 79
BsmBI CGTCTCn'nnnn_
Cuts at: 0 593 783
Size: 593 190
Bsp1286I G_dGCh'C
Cuts at: 0 79 356 386 626 666 776 783
Size: 79 277 30 240 40 110 7
Fragments arranged by size:
277 240 110 79 40 30 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 443 619 783
Size: 443 176 164
Fragments arranged by size:
443 176 164
BstXI CCAn_nnnn'nTGG
Cuts at: 0 651 783
Size: 651 132
BstYI r'GATC_y
Cuts at: 0 23 563 783
Size: 23 540 220
Fragments arranged by size:
540 220 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 82 783
Size: 82 701
DraIII CAC_nnn'GTG
Cuts at: 0 329 783
Size: 329 454
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 328 783
Size: 328 455
EcoICRI GAG'CTC
Cuts at: 0 354 783
Size: 354 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 319 619 783
Size: 50 269 300 164
Fragments arranged by size:
300 269 164 50
EcoO109I rG'GnC_Cy
Cuts at: 0 305 417 783
Size: 305 112 366
Fragments arranged by size:
366 305 112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 329 783
Size: 299 30 454
Fragments arranged by size:
454 299 30
MslI CAynn'nnrTG
Cuts at: 0 82 649 783
Size: 82 567 134
Fragments arranged by size:
567 134 82
MspA1I CmG'CkG
Cuts at: 0 252 372 783
Size: 252 120 411
Fragments arranged by size:
411 252 120
NspI r_CATG'y
Cuts at: 0 138 783
Size: 138 645
PflMI CCAn_nnn'nTGG
Cuts at: 0 567 783
Size: 567 216
PfoI T'CCnGG_A
Cuts at: 0 296 406 783
Size: 296 110 377
Fragments arranged by size:
377 296 110
PmlI CAC'GTG
Cuts at: 0 618 783
Size: 618 165
PpuMI rG'GwC_Cy
Cuts at: 0 305 783
Size: 305 478
SacI G_AGCT'C
Cuts at: 0 356 783
Size: 356 427
ScaI AGT'ACT
Cuts at: 0 274 783
Size: 274 509
SmaI CCC'GGG
Cuts at: 0 310 783
Size: 310 473
StuI AGG'CCT
Cuts at: 0 35 783
Size: 35 748
TatI w'GTAC_w
Cuts at: 0 272 783
Size: 272 511
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 313 345 698 730 783
Size: 313 32 353 32 53
Fragments arranged by size:
353 313 53 32 32
XmaI C'CCGG_G
Cuts at: 0 308 783
Size: 308 475
ZraI GAC'GTC
Cuts at: 0 603 783
Size: 603 180
Enzymes that do cut:
AarI AatII AccI AleI AlfI AlwNI AvaI BamHI
BanII BbvCI BglI BlpI BmgBI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BstXI BstYI BtgZI DraIII EciI
EcoICRI Eco57MI EcoO109I MmeI MslI MspA1I NspI PflMI
PfoI PmlI PpuMI SacI ScaI SmaI StuI TatI
TstI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbsI BcgI BciVI BclI BfrBI
BglII Bme1580I BmrI BmtI BplI BpuEI BsaI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI SexAI SfcI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TaqII Tth111I XbaI XcmI XhoI
XmnI