Restriction map for
pFN21AE1547
(Linear) (Six-base) MAPSORT of: KIEE1547.seq Check: 5536 from: 1 to: 1773
KIEE1547.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 1773
Size: 658 1115
AflII C'TTAA_G
Cuts at: 0 1216 1773
Size: 1216 557
AflIII A'CryG_T
Cuts at: 0 1121 1773
Size: 1121 652
AleI CACnn'nnGTG
Cuts at: 0 1584 1678 1773
Size: 1584 94 95
Fragments arranged by size:
1584 95 94
ApaI G_GGCC'C
Cuts at: 0 1257 1773
Size: 1257 516
ApaLI G'TGCA_C
Cuts at: 0 1577 1773
Size: 1577 196
AvaI C'yCGr_G
Cuts at: 0 110 473 1195 1607 1773
Size: 110 363 722 412 166
Fragments arranged by size:
722 412 363 166 110
BanI G'GyrC_C
Cuts at: 0 30 1078 1680 1773
Size: 30 1048 602 93
Fragments arranged by size:
1048 602 93 30
BanII G_rGCy'C
Cuts at: 0 455 769 1053 1257 1773
Size: 455 314 284 204 516
Fragments arranged by size:
516 455 314 284 204
BbeI G_GCGC'C
Cuts at: 0 34 1773
Size: 34 1739
BbsI GAAGACnn'nnnn_
Cuts at: 0 1751 1773
Size: 1751 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1666 1700 1773
Size: 1666 34 73
Fragments arranged by size:
1666 73 34
BclI T'GATC_A
Cuts at: 0 617 1773
Size: 617 1156
BlpI GC'TnA_GC
Cuts at: 0 454 1773
Size: 454 1319
Bme1580I G_kGCm'C
Cuts at: 0 1257 1581 1773
Size: 1257 324 192
Fragments arranged by size:
1257 324 192
BmgBI CAC'GTC
Cuts at: 0 1124 1773
Size: 1124 649
BmrI ACTGGGnnnn_n'
Cuts at: 0 1437 1527 1773
Size: 1437 90 246
Fragments arranged by size:
1437 246 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 469 1773
Size: 469 1304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 1773
Size: 377 1396
Bpu10I CC'TnA_GC
Cuts at: 0 393 847 1773
Size: 393 454 926
Fragments arranged by size:
926 454 393
BsaBI GATnn'nnATC
Cuts at: 0 965 1773
Size: 965 808
BsaHI Gr'CG_yC
Cuts at: 0 31 1773
Size: 31 1742
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 874 904 1773
Size: 874 30 869
Fragments arranged by size:
874 869 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 730 1773
Size: 730 1043
BsiHKAI G_wGCw'C
Cuts at: 0 455 769 1581 1773
Size: 455 314 812 192
Fragments arranged by size:
812 455 314 192
Bsp1286I G_dGCh'C
Cuts at: 0 455 769 1053 1257 1581 1773
Size: 455 314 284 204 324 192
Fragments arranged by size:
455 324 314 284 204 192
BspHI T'CATG_A
Cuts at: 0 1410 1773
Size: 1410 363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1710 1773
Size: 1710 63
BsrBI CCG'CTC
Cuts at: 0 117 1773
Size: 117 1656
BsrDI GCAATG_nn'
Cuts at: 0 172 1773
Size: 172 1601
BstAPI GCAn_nnn'nTGC
Cuts at: 0 90 1773
Size: 90 1683
BstBI TT'CG_AA
Cuts at: 0 519 1773
Size: 519 1254
BstXI CCAn_nnnn'nTGG
Cuts at: 0 662 1773
Size: 662 1111
BtgI C'CryG_G
Cuts at: 0 1364 1773
Size: 1364 409
BtsI GCAGTG_nn'
Cuts at: 0 32 1511 1773
Size: 32 1479 262
Fragments arranged by size:
1479 262 32
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1736 1771 1773
Size: 1736 35 2
Fragments arranged by size:
1736 35 2
EaeI y'GGCC_r
Cuts at: 0 182 248 1773
Size: 182 66 1525
Fragments arranged by size:
1525 182 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 163 191 1773
Size: 163 28 1582
Fragments arranged by size:
1582 163 28
EcoICRI GAG'CTC
Cuts at: 0 453 767 1773
Size: 453 314 1006
Fragments arranged by size:
1006 453 314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 658 1773
Size: 377 281 1115
Fragments arranged by size:
1115 377 281
EcoNI CCTnn'n_nnAGG
Cuts at: 0 250 1773
Size: 250 1523
EcoO109I rG'GnC_Cy
Cuts at: 0 120 397 720 1773
Size: 120 277 323 1053
Fragments arranged by size:
1053 323 277 120
EcoRV GAT'ATC
Cuts at: 0 349 1470 1773
Size: 349 1121 303
Fragments arranged by size:
1121 349 303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 276 1773
Size: 276 1497
HaeII r_GCGC'y
Cuts at: 0 34 433 1773
Size: 34 399 1340
Fragments arranged by size:
1340 399 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 874 906 1773
Size: 874 32 867
Fragments arranged by size:
874 867 32
HincII GTy'rAC
Cuts at: 0 1164 1421 1773
Size: 1164 257 352
Fragments arranged by size:
1164 352 257
HindIII A'AGCT_T
Cuts at: 0 1405 1565 1773
Size: 1405 160 208
Fragments arranged by size:
1405 208 160
KasI G'GCGC_C
Cuts at: 0 30 1773
Size: 30 1743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 1773
Size: 917 856
MscI TGG'CCA
Cuts at: 0 250 1773
Size: 250 1523
MslI CAynn'nnrTG
Cuts at: 0 550 664 748 1111 1584 1678 1773
Size: 550 114 84 363 473 94 95
Fragments arranged by size:
550 473 363 114 95 94 84
NarI GG'CG_CC
Cuts at: 0 31 1773
Size: 31 1742
NspI r_CATG'y
Cuts at: 0 1153 1287 1773
Size: 1153 134 486
Fragments arranged by size:
1153 486 134
PflMI CCAn_nnn'nTGG
Cuts at: 0 231 1773
Size: 231 1542
PspOMI G'GGCC_C
Cuts at: 0 1253 1773
Size: 1253 520
SacI G_AGCT'C
Cuts at: 0 455 769 1773
Size: 455 314 1004
Fragments arranged by size:
1004 455 314
ScaI AGT'ACT
Cuts at: 0 1537 1773
Size: 1537 236
SfcI C'TryA_G
Cuts at: 0 1176 1642 1773
Size: 1176 466 131
Fragments arranged by size:
1176 466 131
SfoI GGC'GCC
Cuts at: 0 32 1773
Size: 32 1741
SmaI CCC'GGG
Cuts at: 0 112 1609 1773
Size: 112 1497 164
Fragments arranged by size:
1497 164 112
SmlI C'TyrA_G
Cuts at: 0 473 1216 1773
Size: 473 743 557
Fragments arranged by size:
743 557 473
SpeI A'CTAG_T
Cuts at: 0 1326 1773
Size: 1326 447
SphI G_CATG'C
Cuts at: 0 1287 1773
Size: 1287 486
StuI AGG'CCT
Cuts at: 0 256 1624 1773
Size: 256 1368 149
Fragments arranged by size:
1368 256 149
StyI C'CwwG_G
Cuts at: 0 251 1656 1773
Size: 251 1405 117
Fragments arranged by size:
1405 251 117
TatI w'GTAC_w
Cuts at: 0 1103 1535 1773
Size: 1103 432 238
Fragments arranged by size:
1103 432 238
Tth111I GACn'n_nGTC
Cuts at: 0 1425 1773
Size: 1425 348
XhoI C'TCGA_G
Cuts at: 0 473 1773
Size: 473 1300
XmaI C'CCGG_G
Cuts at: 0 110 1607 1773
Size: 110 1497 166
Fragments arranged by size:
1497 166 110
Enzymes that do cut:
AcuI AflII AflIII AleI ApaI ApaLI AvaI BanI
BanII BbeI BbsI BcgI BclI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaBI BsaHI BsaXI BseRI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BstAPI BstBI
BstXI BtgI BtsI CspCI EaeI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI NarI NspI PflMI PspOMI
SacI ScaI SfcI SfoI SmaI SmlI SpeI SphI
StuI StyI TatI Tth111I XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BfrBI BglI BglII BmtI
BpuEI BsaI BsaAI BsaWI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BsrFI BsrGI BssHII BssSI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI
EagI EarI EcoRI FseI FspI FspAI HpaI KpnI
MfeI MluI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SnaBI SrfI SspI SwaI
TaqII TstI XbaI XcmI XmnI ZraI