(Linear) (Six-base) MAPSORT of: KIEE1545.seq  Check: 8892  from: 1  to: 1221



KIEE1545.orf

 With 182 enzymes: SgfI * 

                        September 30, 2014 10:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470    1154    1221 
   Size:        470     684      67

  Fragments arranged by size: 

                 684     470      67
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     277     309     404     436    1221 
   Size:        277      32      95      32     785

  Fragments arranged by size: 

                 785     277      95      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     241     901    1221 
   Size:        241     660     320

  Fragments arranged by size: 

                 660     320     241
 
ApoI r'AATT_y

Cuts at:      0     114     679     701     952    1221 
   Size:        114     565      22     251     269

  Fragments arranged by size: 

                 565     269     251     114      22
 
AseI AT'TA_AT

Cuts at:      0     755    1221 
   Size:        755     466
 
AvaI C'yCGr_G

Cuts at:      0     478    1221 
   Size:        478     743
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1145    1221 
   Size:       1145      76
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     777    1221 
   Size:        777     444
 
BclI T'GATC_A

Cuts at:      0     980    1221 
   Size:        980     241
 
BfrBI ATG'CAT

Cuts at:      0     879    1221 
   Size:        879     342
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209    1221 
   Size:        209    1012
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     295    1221 
   Size:        295     926
 
BsaWI w'CCGG_w

Cuts at:      0     956    1221 
   Size:        956     265
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     277     307    1095    1125    1221 
   Size:        277      30     788      30      96

  Fragments arranged by size: 

                 788     277      96      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1204    1221 
   Size:       1204      17
 
BspHI T'CATG_A

Cuts at:      0     683     944    1221 
   Size:        683     261     277

  Fragments arranged by size: 

                 683     277     261
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     441    1221 
   Size:        441     780
 
BstBI TT'CG_AA

Cuts at:      0     423     699    1221 
   Size:        423     276     522

  Fragments arranged by size: 

                 522     423     276
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     336    1206    1221 
   Size:        336     870      15

  Fragments arranged by size: 

                 870     336      15
 
BstYI r'GATC_y

Cuts at:      0     347     690    1221 
   Size:        347     343     531

  Fragments arranged by size: 

                 531     347     343
 
BtgI C'CryG_G

Cuts at:      0      14     570    1221 
   Size:         14     556     651

  Fragments arranged by size: 

                 651     556      14
 
DraI TTT'AAA

Cuts at:      0     119    1221 
   Size:        119    1102
 
DraIII CAC_nnn'GTG

Cuts at:      0     793    1221 
   Size:        793     428
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470    1154    1221 
   Size:        470     684      67

  Fragments arranged by size: 

                 684     470      67
 
EcoRI G'AATT_C

Cuts at:      0     701     952    1221 
   Size:        701     251     269

  Fragments arranged by size: 

                 701     269     251
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     926     958    1221 
   Size:        926      32     263

  Fragments arranged by size: 

                 926     263      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     644     761    1221 
   Size:        263     381     117     460

  Fragments arranged by size: 

                 460     381     263     117
 
MslI CAynn'nnrTG

Cuts at:      0    1204    1221 
   Size:       1204      17
 
MspA1I CmG'CkG

Cuts at:      0      16    1221 
   Size:         16    1205
 
NsiI A_TGCA'T

Cuts at:      0     881    1221 
   Size:        881     340
 
NspI r_CATG'y

Cuts at:      0     883    1221 
   Size:        883     338
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     277     309    1221 
   Size:        277      32     912

  Fragments arranged by size: 

                 912     277      32
 
PsiI TTA'TAA

Cuts at:      0      23    1036    1221 
   Size:         23    1013     185

  Fragments arranged by size: 

                1013     185      23
 
PstI C_TGCA'G

Cuts at:      0     446    1221 
   Size:        446     775
 
SacII CC_GC'GG

Cuts at:      0      17    1221 
   Size:         17    1204
 
SfcI C'TryA_G

Cuts at:      0     442    1181    1221 
   Size:        442     739      40

  Fragments arranged by size: 

                 739     442      40
 
SmaI CCC'GGG

Cuts at:      0     480    1221 
   Size:        480     741
 
SmlI C'TyrA_G

Cuts at:      0     224    1221 
   Size:        224     997
 
SpeI A'CTAG_T

Cuts at:      0     312    1221 
   Size:        312     909
 
SphI G_CATG'C

Cuts at:      0     883    1221 
   Size:        883     338
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1095    1127    1140    1172    1221 
   Size:       1095      32      13      32      49

  Fragments arranged by size: 

                1095      49      32      32      13
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     333    1221 
   Size:        333     888
 
XmaI C'CCGG_G

Cuts at:      0     478    1221 
   Size:        478     743
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApoI     AseI     AvaI     BbsI    BciVI
     BclI    BfrBI    BpuEI     BsaI    BsaWI    BsaXI    BseRI    BspHI
   BstAPI    BstBI    BstXI    BstYI     BtgI     DraI   DraIII  Eco57MI
    EcoRI    Hin4I     MmeI     MslI   MspA1I     NsiI     NspI     PpiI
     PsiI     PstI    SacII     SfcI     SmaI     SmlI     SpeI     SphI
     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstEII  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmnI
     ZraI