Restriction map for
pFN21AE1543
(Linear) (Six-base) MAPSORT of: KIEE1543.seq Check: 2266 from: 1 to: 1419
KIEE1543.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1268 1419
Size: 1268 151
AccI GT'mk_AC
Cuts at: 0 755 869 1419
Size: 755 114 550
Fragments arranged by size:
755 550 114
AflIII A'CryG_T
Cuts at: 0 345 1419
Size: 345 1074
ApaI G_GGCC'C
Cuts at: 0 1104 1419
Size: 1104 315
BanI G'GyrC_C
Cuts at: 0 156 599 1342 1419
Size: 156 443 743 77
Fragments arranged by size:
743 443 156 77
BanII G_rGCy'C
Cuts at: 0 68 1104 1238 1419
Size: 68 1036 134 181
Fragments arranged by size:
1036 181 134 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 867 1419
Size: 867 552
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 343 377 754 788 1419
Size: 343 34 377 34 631
Fragments arranged by size:
631 377 343 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 266 1419
Size: 266 1153
BglI GCCn_nnn'nGGC
Cuts at: 0 1351 1419
Size: 1351 68
Bme1580I G_kGCm'C
Cuts at: 0 128 324 1104 1419
Size: 128 196 780 315
Fragments arranged by size:
780 315 196 128
BmgBI CAC'GTC
Cuts at: 0 123 1419
Size: 123 1296
BmrI ACTGGGnnnn_n'
Cuts at: 0 408 666 859 1098 1136 1419
Size: 408 258 193 239 38 283
Fragments arranged by size:
408 283 258 239 193 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 583 1317 1358 1419
Size: 485 98 734 41 61
Fragments arranged by size:
734 485 98 61 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 136 1419
Size: 52 84 1283
Fragments arranged by size:
1283 84 52
BsaI GGTCTCn'nnnn_
Cuts at: 0 1006 1419
Size: 1006 413
BsaAI yAC'GTr
Cuts at: 0 1062 1419
Size: 1062 357
BsaBI GATnn'nnATC
Cuts at: 0 449 1419
Size: 449 970
BsaWI w'CCGG_w
Cuts at: 0 326 1419
Size: 326 1093
BseYI C'CCAG_C
Cuts at: 0 1214 1360 1419
Size: 1214 146 59
Fragments arranged by size:
1214 146 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 670 1419
Size: 205 465 749
Fragments arranged by size:
749 465 205
BsiEI CG_ry'CG
Cuts at: 0 243 1419
Size: 243 1176
BsiHKAI G_wGCw'C
Cuts at: 0 68 356 736 1238 1419
Size: 68 288 380 502 181
Fragments arranged by size:
502 380 288 181 68
BsmI GAATG_Cn'
Cuts at: 0 177 1419
Size: 177 1242
Bsp1286I G_dGCh'C
Cuts at: 0 68 128 324 356 736 1104 1238 1419
Size: 68 60 196 32 380 368 134 181
Fragments arranged by size:
380 368 196 181 134 68 60 32
BspHI T'CATG_A
Cuts at: 0 639 1419
Size: 639 780
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1024 1268 1419
Size: 1024 244 151
Fragments arranged by size:
1024 244 151
BsrDI GCAATG_nn'
Cuts at: 0 708 1315 1419
Size: 708 607 104
Fragments arranged by size:
708 607 104
BsrFI r'CCGG_y
Cuts at: 0 98 455 1419
Size: 98 357 964
Fragments arranged by size:
964 357 98
BstAPI GCAn_nnn'nTGC
Cuts at: 0 558 1419
Size: 558 861
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1111 1117 1204 1383 1419
Size: 1111 6 87 179 36
Fragments arranged by size:
1111 179 87 36 6
BtgI C'CryG_G
Cuts at: 0 59 786 1110 1116 1419
Size: 59 727 324 6 303
Fragments arranged by size:
727 324 303 59 6
BtsI GCAGTG_nn'
Cuts at: 0 568 593 1419
Size: 568 25 826
Fragments arranged by size:
826 568 25
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1297 1332 1419
Size: 1297 35 87
Fragments arranged by size:
1297 87 35
DraIII CAC_nnn'GTG
Cuts at: 0 1207 1419
Size: 1207 212
DrdI GACnn_nn'nnGTC
Cuts at: 0 372 511 1419
Size: 372 139 908
Fragments arranged by size:
908 372 139
EaeI y'GGCC_r
Cuts at: 0 96 476 789 1113 1194 1419
Size: 96 380 313 324 81 225
Fragments arranged by size:
380 324 313 225 96 81
EarI CTCTTCn'nnn_
Cuts at: 0 748 1419
Size: 748 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1171 1419
Size: 1171 248
EcoICRI GAG'CTC
Cuts at: 0 66 1236 1419
Size: 66 1170 183
Fragments arranged by size:
1170 183 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 583 1317 1358 1419
Size: 485 98 734 41 61
Fragments arranged by size:
734 485 98 61 41
EcoO109I rG'GnC_Cy
Cuts at: 0 16 822 1364 1419
Size: 16 806 542 55
Fragments arranged by size:
806 542 55 16
FseI GG_CCGG'CC
Cuts at: 0 102 1419
Size: 102 1317
FspI TGC'GCA
Cuts at: 0 427 1419
Size: 427 992
HaeII r_GCGC'y
Cuts at: 0 1125 1419
Size: 1125 294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 916 948 1419
Size: 916 32 471
Fragments arranged by size:
916 471 32
MscI TGG'CCA
Cuts at: 0 478 1115 1196 1419
Size: 478 637 81 223
Fragments arranged by size:
637 478 223 81
MslI CAynn'nnrTG
Cuts at: 0 293 644 1115 1419
Size: 293 351 471 304
Fragments arranged by size:
471 351 304 293
MspA1I CmG'CkG
Cuts at: 0 677 711 967 1218 1277 1336 1419
Size: 677 34 256 251 59 59 83
Fragments arranged by size:
677 256 251 83 59 59 34
NaeI GCC'GGC
Cuts at: 0 100 1419
Size: 100 1319
NcoI C'CATG_G
Cuts at: 0 59 786 1110 1116 1419
Size: 59 727 324 6 303
Fragments arranged by size:
727 324 303 59 6
NgoMIV G'CCGG_C
Cuts at: 0 98 1419
Size: 98 1321
PasI CC'CwG_GG
Cuts at: 0 957 1419
Size: 957 462
PflMI CCAn_nnn'nTGG
Cuts at: 0 474 1419
Size: 474 945
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 1419
Size: 523 32 864
Fragments arranged by size:
864 523 32
PpuMI rG'GwC_Cy
Cuts at: 0 1364 1419
Size: 1364 55
PspOMI G'GGCC_C
Cuts at: 0 1100 1419
Size: 1100 319
PstI C_TGCA'G
Cuts at: 0 341 1265 1419
Size: 341 924 154
Fragments arranged by size:
924 341 154
PvuII CAG'CTG
Cuts at: 0 711 967 1218 1336 1419
Size: 711 256 251 118 83
Fragments arranged by size:
711 256 251 118 83
SacI G_AGCT'C
Cuts at: 0 68 1238 1419
Size: 68 1170 181
Fragments arranged by size:
1170 181 68
SanDI GG'GwC_CC
Cuts at: 0 1364 1419
Size: 1364 55
SexAI A'CCwGG_T
Cuts at: 0 1203 1419
Size: 1203 216
SfcI C'TryA_G
Cuts at: 0 337 891 1261 1419
Size: 337 554 370 158
Fragments arranged by size:
554 370 337 158
SmlI C'TyrA_G
Cuts at: 0 67 151 1419
Size: 67 84 1268
Fragments arranged by size:
1268 84 67
SspI AAT'ATT
Cuts at: 0 222 1090 1419
Size: 222 868 329
Fragments arranged by size:
868 329 222
StuI AGG'CCT
Cuts at: 0 190 251 1354 1419
Size: 190 61 1103 65
Fragments arranged by size:
1103 190 65 61
StyI C'CwwG_G
Cuts at: 0 59 786 1110 1116 1419
Size: 59 727 324 6 303
Fragments arranged by size:
727 324 303 59 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 99 411 856 1099 1419
Size: 99 312 445 243 320
Fragments arranged by size:
445 320 312 243 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 269 301 1419
Size: 269 32 1118
Fragments arranged by size:
1118 269 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 27 1419
Size: 27 1392
Enzymes that do cut:
AarI AccI AflIII ApaI BanI BanII BbsI BcgI
BciVI BglI Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaAI BsaBI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstXI BtgI
BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FseI FspI HaeII Hin4I MscI MslI
MspA1I NaeI NcoI NgoMIV PasI PflMI PpiI PpuMI
PspOMI PstI PvuII SacI SanDI SexAI SfcI SmlI
SspI StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BclI
BfrBI BglII BlpI BmtI BplI Bpu10I BsaHI BsaXI
BseRI BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraI
EagI EcoNI EcoRI EcoRV FalI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI ZraI