(Linear) (Six-base) MAPSORT of: KIEE1542.seq  Check: 7097  from: 1  to: 588



KIEE1542.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:41  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     588 
   Size:        212     376
 
AflIII A'CryG_T

Cuts at:      0     466     588 
   Size:        466     122
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     407     588 
   Size:        407     181
 
AlwNI CAG_nnn'CTG

Cuts at:      0     337     394     588 
   Size:        337      57     194

  Fragments arranged by size: 

                 337     194      57
 
ApoI r'AATT_y

Cuts at:      0     277     588 
   Size:        277     311
 
AvaI C'yCGr_G

Cuts at:      0     425     588 
   Size:        425     163
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     323     588 
   Size:        323     265
 
BglII A'GATC_T

Cuts at:      0     124     588 
   Size:        124     464
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     212     588 
   Size:        212     376
 
BsaAI yAC'GTr

Cuts at:      0     467     588 
   Size:        467     121
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     301     588 
   Size:        301     287
 
BsmI GAATG_Cn'

Cuts at:      0     384     588 
   Size:        384     204
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     575     588 
   Size:        575      13
 
BsrDI GCAATG_nn'

Cuts at:      0     208     588 
   Size:        208     380
 
BstYI r'GATC_y

Cuts at:      0     124     588 
   Size:        124     464
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     453     488     588 
   Size:        453      35     100

  Fragments arranged by size: 

                 453     100      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     356     588 
   Size:        356     232
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     588 
   Size:        212     376
 
EcoRI G'AATT_C

Cuts at:      0     277     588 
   Size:        277     311
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     116     123     148     155     588 
   Size:        116       7      25       7     433

  Fragments arranged by size: 

                 433     116      25       7       7
 
MslI CAynn'nnrTG

Cuts at:      0     389     588 
   Size:        389     199
 
PmlI CAC'GTG

Cuts at:      0     467     588 
   Size:        467     121
 
PstI C_TGCA'G

Cuts at:      0     572     588 
   Size:        572      16
 
SbfI CC_TGCA'GG

Cuts at:      0     572     588 
   Size:        572      16
 
SfcI C'TryA_G

Cuts at:      0     434     568     588 
   Size:        434     134      20

  Fragments arranged by size: 

                 434     134      20
 
SmlI C'TyrA_G

Cuts at:      0     425     588 
   Size:        425     163
 
SspI AAT'ATT

Cuts at:      0     294     411     588 
   Size:        294     117     177

  Fragments arranged by size: 

                 294     177     117
 
StyI C'CwwG_G

Cuts at:      0     170     588 
   Size:        170     418
 
TatI w'GTAC_w

Cuts at:      0     177     588 
   Size:        177     411
 
XbaI T'CTAG_A

Cuts at:      0      60     588 
   Size:         60     528
 
XhoI C'TCGA_G

Cuts at:      0     425     588 
   Size:        425     163
 
XmnI GAAnn'nnTTC

Cuts at:      0     224     588 
   Size:        224     364
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI    AlwNI     ApoI     AvaI     BbsI    BglII
     BsaI    BsaAI    BseRI     BsmI    BspMI    BsrDI    BstYI    CspCI
     EarI  Eco57MI    EcoRI    Hin4I     MslI     PmlI     PstI     SbfI
     SfcI     SmlI     SspI     StyI     TatI     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TstI
  Tth111I     XcmI     XmaI     ZraI