Restriction map for
pFN21AE1541
(Linear) (Six-base) MAPSORT of: KIEE1541.seq Check: 1618 from: 1 to: 1425
KIEE1541.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AccI GT'mk_AC
Cuts at: 0 539 1425
Size: 539 886
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 1425
Size: 877 548
AflIII A'CryG_T
Cuts at: 0 816 1425
Size: 816 609
AleI CACnn'nnGTG
Cuts at: 0 1405 1425
Size: 1405 20
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 230 1425
Size: 230 1195
AlwNI CAG_nnn'CTG
Cuts at: 0 420 1360 1425
Size: 420 940 65
Fragments arranged by size:
940 420 65
ApoI r'AATT_y
Cuts at: 0 1266 1348 1425
Size: 1266 82 77
Fragments arranged by size:
1266 82 77
AseI AT'TA_AT
Cuts at: 0 782 1425
Size: 782 643
BanI G'GyrC_C
Cuts at: 0 315 1425
Size: 315 1110
BanII G_rGCy'C
Cuts at: 0 255 1425
Size: 255 1170
BbsI GAAGACnn'nnnn_
Cuts at: 0 841 1425
Size: 841 584
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 125 159 1425
Size: 125 34 1266
Fragments arranged by size:
1266 125 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 421 1425
Size: 421 1004
BglI GCCn_nnn'nGGC
Cuts at: 0 859 1425
Size: 859 566
BlpI GC'TnA_GC
Cuts at: 0 762 1425
Size: 762 663
BmgBI CAC'GTC
Cuts at: 0 204 1152 1425
Size: 204 948 273
Fragments arranged by size:
948 273 204
BmrI ACTGGGnnnn_n'
Cuts at: 0 552 715 1348 1425
Size: 552 163 633 77
Fragments arranged by size:
633 552 163 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 639 991 1425
Size: 73 566 352 434
Fragments arranged by size:
566 434 352 73
Bpu10I CC'TnA_GC
Cuts at: 0 1258 1361 1425
Size: 1258 103 64
Fragments arranged by size:
1258 103 64
BsaWI w'CCGG_w
Cuts at: 0 607 1425
Size: 607 818
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 680 704 1425
Size: 680 24 721
Fragments arranged by size:
721 680 24
BseYI C'CCAG_C
Cuts at: 0 425 1425
Size: 425 1000
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 226 887 1425
Size: 151 75 661 538
Fragments arranged by size:
661 538 151 75
BsiHKAI G_wGCw'C
Cuts at: 0 255 1425
Size: 255 1170
BsmBI CGTCTCn'nnnn_
Cuts at: 0 194 1425
Size: 194 1231
Bsp1286I G_dGCh'C
Cuts at: 0 255 1425
Size: 255 1170
BsrBI CCG'CTC
Cuts at: 0 653 761 1425
Size: 653 108 664
Fragments arranged by size:
664 653 108
BsrDI GCAATG_nn'
Cuts at: 0 1078 1425
Size: 1078 347
BstEII G'GTnAC_C
Cuts at: 0 1398 1425
Size: 1398 27
BstXI CCAn_nnnn'nTGG
Cuts at: 0 612 1425
Size: 612 813
BtgI C'CryG_G
Cuts at: 0 84 1425
Size: 84 1341
BtsI GCAGTG_nn'
Cuts at: 0 47 888 1425
Size: 47 841 537
Fragments arranged by size:
841 537 47
EcoICRI GAG'CTC
Cuts at: 0 253 1425
Size: 253 1172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 639 877 991 1425
Size: 73 566 238 114 434
Fragments arranged by size:
566 434 238 114 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 701 1328 1425
Size: 701 627 97
Fragments arranged by size:
701 627 97
EcoO109I rG'GnC_Cy
Cuts at: 0 417 631 685 706 1425
Size: 417 214 54 21 719
Fragments arranged by size:
719 417 214 54 21
EcoRI G'AATT_C
Cuts at: 0 1266 1348 1425
Size: 1266 82 77
Fragments arranged by size:
1266 82 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 1425
Size: 46 32 1347
Fragments arranged by size:
1347 46 32
HincII GTy'rAC
Cuts at: 0 280 1071 1425
Size: 280 791 354
Fragments arranged by size:
791 354 280
HindIII A'AGCT_T
Cuts at: 0 298 551 1425
Size: 298 253 874
Fragments arranged by size:
874 298 253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1037 1425
Size: 224 813 388
Fragments arranged by size:
813 388 224
MslI CAynn'nnrTG
Cuts at: 0 1405 1425
Size: 1405 20
MspA1I CmG'CkG
Cuts at: 0 397 958 1425
Size: 397 561 467
Fragments arranged by size:
561 467 397
NdeI CA'TA_TG
Cuts at: 0 126 1425
Size: 126 1299
NspI r_CATG'y
Cuts at: 0 12 81 217 538 808 820 1425
Size: 12 69 136 321 270 12 605
Fragments arranged by size:
605 321 270 136 69 12 12
PasI CC'CwG_GG
Cuts at: 0 420 1425
Size: 420 1005
PciI A'CATG_T
Cuts at: 0 816 1425
Size: 816 609
PflMI CCAn_nnn'nTGG
Cuts at: 0 420 1425
Size: 420 1005
PpuMI rG'GwC_Cy
Cuts at: 0 685 1425
Size: 685 740
PstI C_TGCA'G
Cuts at: 0 581 1425
Size: 581 844
PvuII CAG'CTG
Cuts at: 0 397 958 1425
Size: 397 561 467
Fragments arranged by size:
561 467 397
SacI G_AGCT'C
Cuts at: 0 255 1425
Size: 255 1170
ScaI AGT'ACT
Cuts at: 0 172 1425
Size: 172 1253
SexAI A'CCwGG_T
Cuts at: 0 1016 1425
Size: 1016 409
SfcI C'TryA_G
Cuts at: 0 577 1325 1425
Size: 577 748 100
Fragments arranged by size:
748 577 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 333 417 1425
Size: 333 84 1008
Fragments arranged by size:
1008 333 84
TatI w'GTAC_w
Cuts at: 0 170 512 960 1425
Size: 170 342 448 465
Fragments arranged by size:
465 448 342 170
Tth111I GACn'n_nGTC
Cuts at: 0 544 1425
Size: 544 881
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApoI AseI
BanI BanII BbsI BcgI BciVI BglI BlpI BmgBI
BmrI BpmI Bpu10I BsaWI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BsrBI BsrDI BstEII BstXI BtgI BtsI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII
MmeI MslI MspA1I NdeI NspI PasI PciI PflMI
PpuMI PstI PvuII SacI ScaI SexAI SfcI TaqII
TatI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BclI BfrBI BglII Bme1580I BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsiEI
BsiWI BsmI BspEI BspHI BspMI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoRV FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TstI XbaI XcmI XhoI XmaI
XmnI ZraI