(Linear) (Six-base) MAPSORT of: KIEE1540.seq  Check: 4069  from: 1  to: 375



KIEE1540.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:41  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      39     375 
   Size:         39     336
 
ApoI r'AATT_y

Cuts at:      0     328     375 
   Size:        328      47
 
AvaI C'yCGr_G

Cuts at:      0     155     375 
   Size:        155     220
 
BamHI G'GATC_C

Cuts at:      0      66     375 
   Size:         66     309
 
BbvCI CC'TCA_GC

Cuts at:      0      25     375 
   Size:         25     350
 
Bpu10I CC'TnA_GC

Cuts at:      0      25     375 
   Size:         25     350
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     375 
   Size:         37     338
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25      42     375 
   Size:         25      17     333

  Fragments arranged by size: 

                 333      25      17
 
BstYI r'GATC_y

Cuts at:      0      66     375 
   Size:         66     309
 
EaeI y'GGCC_r

Cuts at:      0      41     375 
   Size:         41     334
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     375 
   Size:         19     356
 
HaeII r_GCGC'y

Cuts at:      0      25     375 
   Size:         25     350
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     244     276     375 
   Size:        244      32      99

  Fragments arranged by size: 

                 244      99      32
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     141     375 
   Size:        141     234
 
PstI C_TGCA'G

Cuts at:      0      53     375 
   Size:         53     322
 
SfcI C'TryA_G

Cuts at:      0      49     375 
   Size:         49     326
 
SmaI CCC'GGG

Cuts at:      0     157     375 
   Size:        157     218
 
SmlI C'TyrA_G

Cuts at:      0      16     375 
   Size:         16     359
 
SrfI GCCC'GGGC

Cuts at:      0     157     375 
   Size:        157     218
 
XmaI C'CCGG_G

Cuts at:      0     155     375 
   Size:        155     220
 

 Enzymes that do cut:

    AlwNI     ApoI     AvaI    BamHI    BbvCI   Bpu10I    BpuEI    BseRI
    BstYI     EaeI     EciI    HaeII    Hin4I    PflMI     PstI     SfcI
     SmaI     SmlI     SrfI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI