Restriction map for
pFN21AE1539
(Linear) (Six-base) MAPSORT of: KIEE1539.seq Check: 7947 from: 1 to: 939
KIEE1539.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 570 939
Size: 85 485 369
Fragments arranged by size:
485 369 85
AfeI AGC'GCT
Cuts at: 0 72 939
Size: 72 867
AflII C'TTAA_G
Cuts at: 0 159 939
Size: 159 780
AflIII A'CryG_T
Cuts at: 0 549 939
Size: 549 390
AleI CACnn'nnGTG
Cuts at: 0 304 939
Size: 304 635
ApaI G_GGCC'C
Cuts at: 0 870 939
Size: 870 69
ApoI r'AATT_y
Cuts at: 0 381 472 767 939
Size: 381 91 295 172
Fragments arranged by size:
381 295 172 91
AseI AT'TA_AT
Cuts at: 0 530 939
Size: 530 409
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 359 392 939
Size: 359 33 547
Fragments arranged by size:
547 359 33
BanII G_rGCy'C
Cuts at: 0 870 939
Size: 870 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 405 939
Size: 405 534
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 136 170 939
Size: 136 34 769
Fragments arranged by size:
769 136 34
BglII A'GATC_T
Cuts at: 0 395 939
Size: 395 544
Bme1580I G_kGCm'C
Cuts at: 0 301 870 939
Size: 301 569 69
Fragments arranged by size:
569 301 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 521 939
Size: 95 426 418
Fragments arranged by size:
426 418 95
BsaAI yAC'GTr
Cuts at: 0 550 939
Size: 550 389
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 939
Size: 755 184
BsiEI CG_ry'CG
Cuts at: 0 30 939
Size: 30 909
Bsp1286I G_dGCh'C
Cuts at: 0 301 870 939
Size: 301 569 69
Fragments arranged by size:
569 301 69
BstYI r'GATC_y
Cuts at: 0 395 939
Size: 395 544
BtgI C'CryG_G
Cuts at: 0 36 875 939
Size: 36 839 64
Fragments arranged by size:
839 64 36
BtsI GCAGTG_nn'
Cuts at: 0 743 939
Size: 743 196
ClaI AT'CG_AT
Cuts at: 0 146 939
Size: 146 793
DraI TTT'AAA
Cuts at: 0 726 939
Size: 726 213
EaeI y'GGCC_r
Cuts at: 0 27 308 939
Size: 27 281 631
Fragments arranged by size:
631 281 27
EagI C'GGCC_G
Cuts at: 0 27 939
Size: 27 912
EarI CTCTTCn'nnn_
Cuts at: 0 61 405 939
Size: 61 344 534
Fragments arranged by size:
534 344 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 95 521 570 939
Size: 85 10 426 49 369
Fragments arranged by size:
426 369 85 49 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 693 939
Size: 693 246
EcoRI G'AATT_C
Cuts at: 0 767 939
Size: 767 172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 174 600 939
Size: 174 426 339
Fragments arranged by size:
426 339 174
HaeII r_GCGC'y
Cuts at: 0 57 74 939
Size: 57 17 865
Fragments arranged by size:
865 57 17
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 507 939
Size: 168 339 432
Fragments arranged by size:
432 339 168
MscI TGG'CCA
Cuts at: 0 310 939
Size: 310 629
MslI CAynn'nnrTG
Cuts at: 0 304 939
Size: 304 635
MspA1I CmG'CkG
Cuts at: 0 38 540 939
Size: 38 502 399
Fragments arranged by size:
502 399 38
NcoI C'CATG_G
Cuts at: 0 875 939
Size: 875 64
PmlI CAC'GTG
Cuts at: 0 550 939
Size: 550 389
PsiI TTA'TAA
Cuts at: 0 793 939
Size: 793 146
PspOMI G'GGCC_C
Cuts at: 0 866 939
Size: 866 73
PstI C_TGCA'G
Cuts at: 0 520 939
Size: 520 419
PvuII CAG'CTG
Cuts at: 0 540 939
Size: 540 399
SacII CC_GC'GG
Cuts at: 0 39 939
Size: 39 900
SapI GCTCTTCn'nnn_
Cuts at: 0 61 939
Size: 61 878
ScaI AGT'ACT
Cuts at: 0 326 939
Size: 326 613
SfcI C'TryA_G
Cuts at: 0 516 762 939
Size: 516 246 177
Fragments arranged by size:
516 246 177
SmlI C'TyrA_G
Cuts at: 0 159 939
Size: 159 780
SspI AAT'ATT
Cuts at: 0 789 939
Size: 789 150
StyI C'CwwG_G
Cuts at: 0 262 599 875 939
Size: 262 337 276 64
Fragments arranged by size:
337 276 262 64
SwaI ATTT'AAAT
Cuts at: 0 726 939
Size: 726 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 322 663 939
Size: 322 341 276
Fragments arranged by size:
341 322 276
TatI w'GTAC_w
Cuts at: 0 324 939
Size: 324 615
XmnI GAAnn'nnTTC
Cuts at: 0 583 926 939
Size: 583 343 13
Fragments arranged by size:
583 343 13
Enzymes that do cut:
AcuI AfeI AflII AflIII AleI ApaI ApoI AseI
BaeI BanII BbsI BcgI BglII Bme1580I BpmI BsaAI
BsgI BsiEI Bsp1286I BstYI BtgI BtsI ClaI DraI
EaeI EagI EarI Eco57MI EcoNI EcoRI FalI HaeII
MmeI MscI MslI MspA1I NcoI PmlI PsiI PspOMI
PstI PvuII SacII SapI ScaI SfcI SmlI SspI
StyI SwaI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI AlfI
AloI AlwNI ApaLI AscI AsiSI AvaI AvrII BamHI
BanI BbeI BbvCI BciVI BclI BfrBI BglI BlpI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI CspCI DraIII DrdI EciI EcoICRI EcoO109I EcoRV
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspXI PsrI PvuI
RsrII SacI SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI