Restriction map for
pFN21AE1537
(Linear) (Six-base) MAPSORT of: KIEE1537.seq Check: 3171 from: 1 to: 2736
KIEE1537.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 141 2736
Size: 141 2595
AatII G_ACGT'C
Cuts at: 0 2392 2736
Size: 2392 344
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1681 2521 2736
Size: 1681 840 215
Fragments arranged by size:
1681 840 215
AhdI GACnn_n'nnGTC
Cuts at: 0 714 2736
Size: 714 2022
AleI CACnn'nnGTG
Cuts at: 0 1962 2736
Size: 1962 774
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1794 2122 2736
Size: 1794 328 614
Fragments arranged by size:
1794 614 328
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1588 1620 2736
Size: 1588 32 1116
Fragments arranged by size:
1588 1116 32
AlwNI CAG_nnn'CTG
Cuts at: 0 144 513 1018 1692 2511 2736
Size: 144 369 505 674 819 225
Fragments arranged by size:
819 674 505 369 225 144
ApaI G_GGCC'C
Cuts at: 0 265 1060 1957 2736
Size: 265 795 897 779
Fragments arranged by size:
897 795 779 265
ApoI r'AATT_y
Cuts at: 0 1790 2668 2736
Size: 1790 878 68
Fragments arranged by size:
1790 878 68
AvaI C'yCGr_G
Cuts at: 0 14 563 926 1123 2328 2339 2455 2549
Size: 14 549 363 197 1205 11 116 94
Cuts at: 2549 2736
Size: 187
Fragments arranged by size:
1205 549 363 197 187 116 94 14
11
BamHI G'GATC_C
Cuts at: 0 2278 2736
Size: 2278 458
BanI G'GyrC_C
Cuts at: 0 161 333 1080 1643 1859 2736
Size: 161 172 747 563 216 877
Fragments arranged by size:
877 747 563 216 172 161
BanII G_rGCy'C
Cuts at: 0 256 265 836 948 1060 1123 1400 1516
Size: 256 9 571 112 112 63 277 116
Cuts at: 1516 1745 1957 2590 2736
Size: 229 212 633 146
Fragments arranged by size:
633 571 277 256 229 212 146 116
112 112 63 9
BbeI G_GCGC'C
Cuts at: 0 165 1084 1863 2736
Size: 165 919 779 873
Fragments arranged by size:
919 873 779 165
BbsI GAAGACnn'nnnn_
Cuts at: 0 11 322 2534 2736
Size: 11 311 2212 202
Fragments arranged by size:
2212 311 202 11
BbvCI CC'TCA_GC
Cuts at: 0 77 1508 1837 2353 2736
Size: 77 1431 329 516 383
Fragments arranged by size:
1431 516 383 329 77
BglI GCCn_nnn'nGGC
Cuts at: 0 19 280 2459 2736
Size: 19 261 2179 277
Fragments arranged by size:
2179 277 261 19
BlpI GC'TnA_GC
Cuts at: 0 793 1903 2623 2736
Size: 793 1110 720 113
Fragments arranged by size:
1110 793 720 113
Bme1580I G_kGCm'C
Cuts at: 0 265 1060 1957 2736
Size: 265 795 897 779
Fragments arranged by size:
897 795 779 265
BmgBI CAC'GTC
Cuts at: 0 1482 1803 1964 2736
Size: 1482 321 161 772
Fragments arranged by size:
1482 772 321 161
BmrI ACTGGGnnnn_n'
Cuts at: 0 268 2736
Size: 268 2468
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 129 2365 2736
Size: 129 2236 371
Fragments arranged by size:
2236 371 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 166 331 481 544 622 986 1051
Size: 124 42 165 150 63 78 364 65
Cuts at: 1051 1090 1151 1183 2017 2378 2671 2736
Size: 39 61 32 834 361 293 65
Fragments arranged by size:
834 364 361 293 165 150 124 78
65 65 63 61 42 39 32
Bpu10I CC'TnA_GC
Cuts at: 0 77 1508 1837 2312 2353 2584 2736
Size: 77 1431 329 475 41 231 152
Fragments arranged by size:
1431 475 329 231 152 77 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1828 2044 2736
Size: 1828 216 692
Fragments arranged by size:
1828 692 216
BsaI GGTCTCn'nnnn_
Cuts at: 0 2629 2676 2736
Size: 2629 47 60
Fragments arranged by size:
2629 60 47
BsaAI yAC'GTr
Cuts at: 0 195 576 1293 2736
Size: 195 381 717 1443
Fragments arranged by size:
1443 717 381 195
BsaHI Gr'CG_yC
Cuts at: 0 162 496 1022 1081 1860 2083 2389 2736
Size: 162 334 526 59 779 223 306 347
Fragments arranged by size:
779 526 347 334 306 223 162 59
BsaWI w'CCGG_w
Cuts at: 0 734 899 932 2736
Size: 734 165 33 1804
Fragments arranged by size:
1804 734 165 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 373 403 2736
Size: 373 30 2333
Fragments arranged by size:
2333 373 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 619 1378 1483 1524 2341 2537 2647 2668
Size: 619 759 105 41 817 196 110 21
Cuts at: 2668 2677 2736
Size: 9 59
Fragments arranged by size:
817 759 619 196 110 105 59 41
21 9
BseYI C'CCAG_C
Cuts at: 0 165 249 518 1035 1384 2323 2589 2720
Size: 165 84 269 517 349 939 266 131
Cuts at: 2720 2736
Size: 16
Fragments arranged by size:
939 517 349 269 266 165 131 84
16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 961 1204 1297 1639 2715 2736
Size: 436 525 243 93 342 1076 21
Fragments arranged by size:
1076 525 436 342 243 93 21
BsiEI CG_ry'CG
Cuts at: 0 763 1156 1428 2736
Size: 763 393 272 1308
Fragments arranged by size:
1308 763 393 272
BsiHKAI G_wGCw'C
Cuts at: 0 1400 2259 2736
Size: 1400 859 477
Fragments arranged by size:
1400 859 477
BsmI GAATG_Cn'
Cuts at: 0 1348 1410 2736
Size: 1348 62 1326
Fragments arranged by size:
1348 1326 62
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1472 2380 2396 2736
Size: 1472 908 16 340
Fragments arranged by size:
1472 908 340 16
Bsp1286I G_dGCh'C
Cuts at: 0 256 265 836 948 1060 1123 1400 1516
Size: 256 9 571 112 112 63 277 116
Cuts at: 1516 1745 1957 2259 2590 2736
Size: 229 212 302 331 146
Fragments arranged by size:
571 331 302 277 256 229 212 146
116 112 112 63 9
BspEI T'CCGG_A
Cuts at: 0 734 2736
Size: 734 2002
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 141 429 1841 2027 2627 2736
Size: 141 288 1412 186 600 109
Fragments arranged by size:
1412 600 288 186 141 109
BsrBI CCG'CTC
Cuts at: 0 570 585 2049 2241 2736
Size: 570 15 1464 192 495
Fragments arranged by size:
1464 570 495 192 15
BsrDI GCAATG_nn'
Cuts at: 0 2379 2736
Size: 2379 357
BsrFI r'CCGG_y
Cuts at: 0 336 452 755 2736
Size: 336 116 303 1981
Fragments arranged by size:
1981 336 303 116
BsrGI T'GTAC_A
Cuts at: 0 671 2736
Size: 671 2065
BssSI C'ACGA_G
Cuts at: 0 1948 2736
Size: 1948 788
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1227 2004 2736
Size: 1227 777 732
Fragments arranged by size:
1227 777 732
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1964 2736
Size: 1964 772
BstYI r'GATC_y
Cuts at: 0 2278 2736
Size: 2278 458
BtgI C'CryG_G
Cuts at: 0 446 650 1334 1639 1676 2474 2736
Size: 446 204 684 305 37 798 262
Fragments arranged by size:
798 684 446 305 262 204 37
BtsI GCAGTG_nn'
Cuts at: 0 1994 2736
Size: 1994 742
EaeI y'GGCC_r
Cuts at: 0 2153 2379 2736
Size: 2153 226 357
Fragments arranged by size:
2153 357 226
EarI CTCTTCn'nnn_
Cuts at: 0 113 1172 1327 1733 2736
Size: 113 1059 155 406 1003
Fragments arranged by size:
1059 1003 406 155 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 595 2124 2536 2736
Size: 595 1529 412 200
Fragments arranged by size:
1529 595 412 200
EcoICRI GAG'CTC
Cuts at: 0 1398 2736
Size: 1398 1338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 166 331 481 544 622 986 1051
Size: 124 42 165 150 63 78 364 65
Cuts at: 1051 1090 1151 1183 1681 2017 2378 2521 2671
Size: 39 61 32 498 336 361 143 150
Cuts at: 2671 2736
Size: 65
Fragments arranged by size:
498 364 361 336 165 150 150 143
124 78 65 65 63 61 42 39
32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 148 1465 2736
Size: 148 1317 1271
Fragments arranged by size:
1317 1271 148
EcoO109I rG'GnC_Cy
Cuts at: 0 34 217 262 1056 1596 1830 1953 2452
Size: 34 183 45 794 540 234 123 499
Cuts at: 2452 2542 2565 2736
Size: 90 23 171
Fragments arranged by size:
794 540 499 234 183 171 123 90
45 34 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 300 2736
Size: 300 2436
FspI TGC'GCA
Cuts at: 0 1297 2736
Size: 1297 1439
FspAI rTGC'GCAy
Cuts at: 0 1297 2736
Size: 1297 1439
HaeII r_GCGC'y
Cuts at: 0 165 1084 1863 2040 2736
Size: 165 919 779 177 696
Fragments arranged by size:
919 779 696 177 165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 700 732 909 941 2736
Size: 373 32 295 32 177 32 1795
Fragments arranged by size:
1795 373 295 177 32 32 32
HincII GTy'rAC
Cuts at: 0 657 1806 2736
Size: 657 1149 930
Fragments arranged by size:
1149 930 657
KasI G'GCGC_C
Cuts at: 0 161 1080 1859 2736
Size: 161 919 779 877
Fragments arranged by size:
919 877 779 161
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 2216 2639 2736
Size: 743 1473 423 97
Fragments arranged by size:
1473 743 423 97
MscI TGG'CCA
Cuts at: 0 2155 2736
Size: 2155 581
MslI CAynn'nnrTG
Cuts at: 0 490 1405 1681 1962 2736
Size: 490 915 276 281 774
Fragments arranged by size:
915 774 490 281 276
MspA1I CmG'CkG
Cuts at: 0 322 405 459 522 818 1641 1659 1689
Size: 322 83 54 63 296 823 18 30
Cuts at: 1689 1857 2398 2649 2736
Size: 168 541 251 87
Fragments arranged by size:
823 541 322 296 251 168 87 83
63 54 30 18
NaeI GCC'GGC
Cuts at: 0 454 2736
Size: 454 2282
NarI GG'CG_CC
Cuts at: 0 162 1081 1860 2736
Size: 162 919 779 876
Fragments arranged by size:
919 876 779 162
NcoI C'CATG_G
Cuts at: 0 1334 2736
Size: 1334 1402
NgoMIV G'CCGG_C
Cuts at: 0 452 2736
Size: 452 2284
NspI r_CATG'y
Cuts at: 0 241 249 678 1302 2002 2736
Size: 241 8 429 624 700 734
Fragments arranged by size:
734 700 624 429 241 8
PasI CC'CwG_GG
Cuts at: 0 500 2569 2736
Size: 500 2069 167
Fragments arranged by size:
2069 500 167
PflMI CCAn_nnn'nTGG
Cuts at: 0 144 177 1335 2736
Size: 144 33 1158 1401
Fragments arranged by size:
1401 1158 144 33
PfoI T'CCnGG_A
Cuts at: 0 287 308 803 2609 2736
Size: 287 21 495 1806 127
Fragments arranged by size:
1806 495 287 127 21
PmlI CAC'GTG
Cuts at: 0 195 576 1293 2736
Size: 195 381 717 1443
Fragments arranged by size:
1443 717 381 195
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2336 2368 2736
Size: 2336 32 368
Fragments arranged by size:
2336 368 32
PpuMI rG'GwC_Cy
Cuts at: 0 217 1596 1830 2565 2736
Size: 217 1379 234 735 171
Fragments arranged by size:
1379 735 234 217 171
PspOMI G'GGCC_C
Cuts at: 0 261 1056 1953 2736
Size: 261 795 897 783
Fragments arranged by size:
897 795 783 261
PstI C_TGCA'G
Cuts at: 0 191 206 1373 1549 1658 1718 2446 2736
Size: 191 15 1167 176 109 60 728 290
Fragments arranged by size:
1167 728 290 191 176 109 60 15
PvuI CG_AT'CG
Cuts at: 0 1428 2736
Size: 1428 1308
PvuII CAG'CTG
Cuts at: 0 459 522 1659 2398 2649 2736
Size: 459 63 1137 739 251 87
Fragments arranged by size:
1137 739 459 251 87 63
RsrII CG'GwC_CG
Cuts at: 0 2408 2736
Size: 2408 328
SacI G_AGCT'C
Cuts at: 0 1400 2736
Size: 1400 1336
SacII CC_GC'GG
Cuts at: 0 1642 2736
Size: 1642 1094
SanDI GG'GwC_CC
Cuts at: 0 2565 2736
Size: 2565 171
SapI GCTCTTCn'nnn_
Cuts at: 0 113 1172 1733 2736
Size: 113 1059 561 1003
Fragments arranged by size:
1059 1003 561 113
SexAI A'CCwGG_T
Cuts at: 0 1011 2736
Size: 1011 1725
SfcI C'TryA_G
Cuts at: 0 187 202 893 1369 1545 1654 1714 2442
Size: 187 15 691 476 176 109 60 728
Cuts at: 2442 2736
Size: 294
Fragments arranged by size:
728 691 476 294 187 176 109 60
15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 280 2459 2736
Size: 280 2179 277
Fragments arranged by size:
2179 280 277
SfoI GGC'GCC
Cuts at: 0 163 1082 1861 2736
Size: 163 919 779 875
Fragments arranged by size:
919 875 779 163
SmaI CCC'GGG
Cuts at: 0 565 2736
Size: 565 2171
SmlI C'TyrA_G
Cuts at: 0 1843 2059 2549 2736
Size: 1843 216 490 187
Fragments arranged by size:
1843 490 216 187
SphI G_CATG'C
Cuts at: 0 241 1302 2736
Size: 241 1061 1434
Fragments arranged by size:
1434 1061 241
StuI AGG'CCT
Cuts at: 0 283 2736
Size: 283 2453
StyI C'CwwG_G
Cuts at: 0 266 684 1076 1334 1358 2736
Size: 266 418 392 258 24 1378
Fragments arranged by size:
1378 418 392 266 258 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 560 2425 2736
Size: 560 1865 311
Fragments arranged by size:
1865 560 311
TatI w'GTAC_w
Cuts at: 0 671 2736
Size: 671 2065
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 687 719 970 1002 2736
Size: 687 32 251 32 1734
Fragments arranged by size:
1734 687 251 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2232 2736
Size: 2232 504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 483 1938 2646 2736
Size: 483 1455 708 90
Fragments arranged by size:
1455 708 483 90
XhoI C'TCGA_G
Cuts at: 0 2549 2736
Size: 2549 187
XmaI C'CCGG_G
Cuts at: 0 563 2736
Size: 563 2173
XmnI GAAnn'nnTTC
Cuts at: 0 283 730 1378 2736
Size: 283 447 648 1358
Fragments arranged by size:
1358 648 447 283
ZraI GAC'GTC
Cuts at: 0 2390 2736
Size: 2390 346
Enzymes that do cut:
AarI AatII AcuI AhdI AleI AlfI AloI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI
BstYI BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SanDI
SapI SexAI SfcI SfiI SfoI SmaI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI ApaLI
AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI
BfrBI BglII BmtI BsaBI BsiWI BspHI BssHII BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoRI EcoRV FseI HindIII HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PshAI PsiI PspXI PsrI SalI SbfI
ScaI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
XbaI