Restriction map for
pFN21AE1535
(Linear) (Six-base) MAPSORT of: KIEE1535.seq Check: 1279 from: 1 to: 2331
KIEE1535.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 307 1246 1761 2331
Size: 295 12 939 515 570
Fragments arranged by size:
939 570 515 295 12
AflIII A'CryG_T
Cuts at: 0 174 1904 2331
Size: 174 1730 427
Fragments arranged by size:
1730 427 174
AleI CACnn'nnGTG
Cuts at: 0 1108 1255 2331
Size: 1108 147 1076
Fragments arranged by size:
1108 1076 147
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2008 2040 2331
Size: 2008 32 291
Fragments arranged by size:
2008 291 32
AlwNI CAG_nnn'CTG
Cuts at: 0 57 1224 1359 1539 1605 2331
Size: 57 1167 135 180 66 726
Fragments arranged by size:
1167 726 180 135 66 57
ApoI r'AATT_y
Cuts at: 0 138 430 2331
Size: 138 292 1901
Fragments arranged by size:
1901 292 138
AvaI C'yCGr_G
Cuts at: 0 703 1439 1749 2331
Size: 703 736 310 582
Fragments arranged by size:
736 703 582 310
BanI G'GyrC_C
Cuts at: 0 16 945 2226 2331
Size: 16 929 1281 105
Fragments arranged by size:
1281 929 105 16
BanII G_rGCy'C
Cuts at: 0 368 471 641 881 977 1036 1804 2331
Size: 368 103 170 240 96 59 768 527
Fragments arranged by size:
768 527 368 240 170 103 96 59
BbeI G_GCGC'C
Cuts at: 0 949 2331
Size: 949 1382
BbvCI CC'TCA_GC
Cuts at: 0 488 897 956 2331
Size: 488 409 59 1375
Fragments arranged by size:
1375 488 409 59
BglI GCCn_nnn'nGGC
Cuts at: 0 1444 1501 1787 1927 1945 2029 2331
Size: 1444 57 286 140 18 84 302
Fragments arranged by size:
1444 302 286 140 84 57 18
BglII A'GATC_T
Cuts at: 0 89 2331
Size: 89 2242
Bme1580I G_kGCm'C
Cuts at: 0 419 1058 1094 1458 2331
Size: 419 639 36 364 873
Fragments arranged by size:
873 639 419 364 36
BmgBI CAC'GTC
Cuts at: 0 177 420 2331
Size: 177 243 1911
Fragments arranged by size:
1911 243 177
BmrI ACTGGGnnnn_n'
Cuts at: 0 486 1011 1934 2331
Size: 486 525 923 397
Fragments arranged by size:
923 525 486 397
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1155 1753 2331
Size: 1155 598 578
Fragments arranged by size:
1155 598 578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1381 1561 1594 1660 1693 2331
Size: 214 1167 180 33 66 33 638
Fragments arranged by size:
1167 638 214 180 66 33 33
Bpu10I CC'TnA_GC
Cuts at: 0 488 897 956 971 2331
Size: 488 409 59 15 1360
Fragments arranged by size:
1360 488 409 59 15
BsaAI yAC'GTr
Cuts at: 0 854 2331
Size: 854 1477
BsaHI Gr'CG_yC
Cuts at: 0 946 2331
Size: 946 1385
BsaWI w'CCGG_w
Cuts at: 0 356 992 2331
Size: 356 636 1339
Fragments arranged by size:
1339 636 356
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2008 2027 2038 2057 2331
Size: 2008 19 11 19 274
Fragments arranged by size:
2008 274 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 790 793 796 838 885 1033 1219
Size: 283 507 3 3 42 47 148 186
Cuts at: 1219 1513 2331
Size: 294 818
Fragments arranged by size:
818 507 294 283 186 148 47 42
3 3
BseYI C'CCAG_C
Cuts at: 0 737 1703 1768 1858 1867 1885 1911 2180
Size: 737 966 65 90 9 18 26 269
Cuts at: 2180 2246 2267 2312 2331
Size: 66 21 45 19
Fragments arranged by size:
966 737 269 90 66 65 45 26
21 19 18 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1058 1600 2331
Size: 532 526 542 731
Fragments arranged by size:
731 542 532 526
BsiEI CG_ry'CG
Cuts at: 0 1313 2331
Size: 1313 1018
BsiHKAI G_wGCw'C
Cuts at: 0 977 1319 1472 1739 2331
Size: 977 342 153 267 592
Fragments arranged by size:
977 592 342 267 153
BsmI GAATG_Cn'
Cuts at: 0 499 2331
Size: 499 1832
BsmBI CGTCTCn'nnnn_
Cuts at: 0 34 754 2174 2331
Size: 34 720 1420 157
Fragments arranged by size:
1420 720 157 34
Bsp1286I G_dGCh'C
Cuts at: 0 368 419 471 641 881 977 1036 1058
Size: 368 51 52 170 240 96 59 22
Cuts at: 1058 1094 1319 1458 1472 1739 1804 2331
Size: 36 225 139 14 267 65 527
Fragments arranged by size:
527 368 267 240 225 170 139 96
65 59 52 51 36 22 14
BspEI T'CCGG_A
Cuts at: 0 356 2331
Size: 356 1975
BspHI T'CATG_A
Cuts at: 0 237 401 2141 2331
Size: 237 164 1740 190
Fragments arranged by size:
1740 237 190 164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 290 2331
Size: 290 2041
BsrBI CCG'CTC
Cuts at: 0 1626 2331
Size: 1626 705
BsrFI r'CCGG_y
Cuts at: 0 1068 1786 1833 2292 2331
Size: 1068 718 47 459 39
Fragments arranged by size:
1068 718 459 47 39
BsrGI T'GTAC_A
Cuts at: 0 1820 2331
Size: 1820 511
BssHII G'CGCG_C
Cuts at: 0 1668 1670 2331
Size: 1668 2 661
Fragments arranged by size:
1668 661 2
BssSI C'ACGA_G
Cuts at: 0 1213 2331
Size: 1213 1118
BstAPI GCAn_nnn'nTGC
Cuts at: 0 130 2331
Size: 130 2201
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1110 1113 2079 2331
Size: 1110 3 966 252
Fragments arranged by size:
1110 966 252 3
BstYI r'GATC_y
Cuts at: 0 89 320 1427 1520 2331
Size: 89 231 1107 93 811
Fragments arranged by size:
1107 811 231 93 89
BtgI C'CryG_G
Cuts at: 0 263 2078 2108 2261 2331
Size: 263 1815 30 153 70
Fragments arranged by size:
1815 263 153 70 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 458 1448 2178 2331
Size: 458 990 730 153
Fragments arranged by size:
990 730 458 153
BtsI GCAGTG_nn'
Cuts at: 0 540 1871 2331
Size: 540 1331 460
Fragments arranged by size:
1331 540 460
DraIII CAC_nnn'GTG
Cuts at: 0 1818 2331
Size: 1818 513
EaeI y'GGCC_r
Cuts at: 0 225 248 1310 1592 2048 2331
Size: 225 23 1062 282 456 283
Fragments arranged by size:
1062 456 283 282 225 23
EagI C'GGCC_G
Cuts at: 0 1310 2331
Size: 1310 1021
EarI CTCTTCn'nnn_
Cuts at: 0 283 930 1264 1330 1460 2331
Size: 283 647 334 66 130 871
Fragments arranged by size:
871 647 334 283 130 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 94 835 889 2331
Size: 94 741 54 1442
Fragments arranged by size:
1442 741 94 54
EcoICRI GAG'CTC
Cuts at: 0 975 2331
Size: 975 1356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 295 307 1246 1381 1561 1594 1660
Size: 214 81 12 939 135 180 33 66
Cuts at: 1660 1693 1761 2331
Size: 33 68 570
Fragments arranged by size:
939 570 214 180 135 81 68 66
33 33 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 37 334 772 1085 2331
Size: 37 297 438 313 1246
Fragments arranged by size:
1246 438 313 297 37
EcoO109I rG'GnC_Cy
Cuts at: 0 279 2031 2331
Size: 279 1752 300
Fragments arranged by size:
1752 300 279
EcoRI G'AATT_C
Cuts at: 0 138 430 2331
Size: 138 292 1901
Fragments arranged by size:
1901 292 138
HaeII r_GCGC'y
Cuts at: 0 805 904 949 1234 1450 1512 1518 1605
Size: 805 99 45 285 216 62 6 87
Cuts at: 1605 2331
Size: 726
Fragments arranged by size:
805 726 285 216 99 87 62 45
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 1125 1157 2331
Size: 61 32 1032 32 1174
Fragments arranged by size:
1174 1032 61 32 32
HincII GTy'rAC
Cuts at: 0 159 2331
Size: 159 2172
HindIII A'AGCT_T
Cuts at: 0 1480 2331
Size: 1480 851
KasI G'GCGC_C
Cuts at: 0 945 2331
Size: 945 1386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 2331
Size: 41 2290
MscI TGG'CCA
Cuts at: 0 227 250 1594 2331
Size: 227 23 1344 737
Fragments arranged by size:
1344 737 227 23
MslI CAynn'nnrTG
Cuts at: 0 406 1108 1255 2331
Size: 406 702 147 1076
Fragments arranged by size:
1076 702 406 147
MspA1I CmG'CkG
Cuts at: 0 14 503 654 741 792 1356 1377 1527
Size: 14 489 151 87 51 564 21 150
Cuts at: 1527 1590 1846 2005 2331
Size: 63 256 159 326
Fragments arranged by size:
564 489 326 256 159 151 150 87
63 51 21 14
NaeI GCC'GGC
Cuts at: 0 1070 1788 1835 2294 2331
Size: 1070 718 47 459 37
Fragments arranged by size:
1070 718 459 47 37
NarI GG'CG_CC
Cuts at: 0 946 2331
Size: 946 1385
NcoI C'CATG_G
Cuts at: 0 263 2078 2108 2331
Size: 263 1815 30 223
Fragments arranged by size:
1815 263 223 30
NgoMIV G'CCGG_C
Cuts at: 0 1068 1786 1833 2292 2331
Size: 1068 718 47 459 39
Fragments arranged by size:
1068 718 459 47 39
NspI r_CATG'y
Cuts at: 0 352 1284 1347 1866 1908 2133 2331
Size: 352 932 63 519 42 225 198
Fragments arranged by size:
932 519 352 225 198 63 42
PciI A'CATG_T
Cuts at: 0 1904 2331
Size: 1904 427
PflMI CCAn_nnn'nTGG
Cuts at: 0 1539 2019 2223 2331
Size: 1539 480 204 108
Fragments arranged by size:
1539 480 204 108
PfoI T'CCnGG_A
Cuts at: 0 383 1127 2331
Size: 383 744 1204
Fragments arranged by size:
1204 744 383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1168 1200 2008 2040 2331
Size: 1168 32 808 32 291
Fragments arranged by size:
1168 808 291 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 279 2331
Size: 279 2052
PshAI GACnn'nnGTC
Cuts at: 0 846 2331
Size: 846 1485
PstI C_TGCA'G
Cuts at: 0 508 773 791 1238 1412 1610 2331
Size: 508 265 18 447 174 198 721
Fragments arranged by size:
721 508 447 265 198 174 18
PvuII CAG'CTG
Cuts at: 0 654 741 792 1356 1377 1527 1590 2331
Size: 654 87 51 564 21 150 63 741
Fragments arranged by size:
741 654 564 150 87 63 51 21
SacI G_AGCT'C
Cuts at: 0 977 2331
Size: 977 1354
SapI GCTCTTCn'nnn_
Cuts at: 0 1460 2331
Size: 1460 871
SbfI CC_TGCA'GG
Cuts at: 0 773 1610 2331
Size: 773 837 721
Fragments arranged by size:
837 773 721
ScaI AGT'ACT
Cuts at: 0 589 2331
Size: 589 1742
SfcI C'TryA_G
Cuts at: 0 504 769 787 1234 1408 1606 2331
Size: 504 265 18 447 174 198 725
Fragments arranged by size:
725 504 447 265 198 174 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1945 2029 2331
Size: 1945 84 302
Fragments arranged by size:
1945 302 84
SfoI GGC'GCC
Cuts at: 0 947 2331
Size: 947 1384
SmaI CCC'GGG
Cuts at: 0 1441 2331
Size: 1441 890
SphI G_CATG'C
Cuts at: 0 352 1347 1866 2331
Size: 352 995 519 465
Fragments arranged by size:
995 519 465 352
StuI AGG'CCT
Cuts at: 0 340 2331
Size: 340 1991
StyI C'CwwG_G
Cuts at: 0 108 263 869 2078 2108 2331
Size: 108 155 606 1209 30 223
Fragments arranged by size:
1209 606 223 155 108 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 468 2331
Size: 468 1863
TatI w'GTAC_w
Cuts at: 0 371 587 920 1394 1820 2331
Size: 371 216 333 474 426 511
Fragments arranged by size:
511 474 426 371 333 216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2027 2059 2331
Size: 2027 32 272
Fragments arranged by size:
2027 272 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 375 1110 2085 2331
Size: 375 735 975 246
Fragments arranged by size:
975 735 375 246
XmaI C'CCGG_G
Cuts at: 0 1439 2331
Size: 1439 892
XmnI GAAnn'nnTTC
Cuts at: 0 576 2331
Size: 576 1755
Enzymes that do cut:
AcuI AflIII AleI AloI AlwNI ApoI AvaI BanI
BanII BbeI BbvCI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PpiI PpuMI PshAI PstI PvuII SacI SapI SbfI
ScaI SfcI SfiI SfoI SmaI SphI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BfrBI BlpI
BmtI BpuEI BsaI BsaBI BsiWI BsrDI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRV FalI
FseI FspI FspAI HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SexAI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XhoI ZraI