Restriction map for
pFN21AE1533
(Linear) (Six-base) MAPSORT of: KIEE1533.seq Check: 1504 from: 1 to: 2313
KIEE1533.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 254 466 664 2313
Size: 226 28 212 198 1649
Fragments arranged by size:
1649 226 212 198 28
AflII C'TTAA_G
Cuts at: 0 889 2313
Size: 889 1424
AflIII A'CryG_T
Cuts at: 0 516 2275 2313
Size: 516 1759 38
Fragments arranged by size:
1759 516 38
AgeI A'CCGG_T
Cuts at: 0 2216 2313
Size: 2216 97
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1118 2313
Size: 1118 1195
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1055 1087 2313
Size: 1055 32 1226
Fragments arranged by size:
1226 1055 32
AlwNI CAG_nnn'CTG
Cuts at: 0 530 1136 1530 2313
Size: 530 606 394 783
Fragments arranged by size:
783 606 530 394
ApoI r'AATT_y
Cuts at: 0 560 1253 1267 1986 2313
Size: 560 693 14 719 327
Fragments arranged by size:
719 693 560 327 14
AvaI C'yCGr_G
Cuts at: 0 689 2313
Size: 689 1624
BamHI G'GATC_C
Cuts at: 0 380 2110 2313
Size: 380 1730 203
Fragments arranged by size:
1730 380 203
BanII G_rGCy'C
Cuts at: 0 690 696 1307 1496 2313
Size: 690 6 611 189 817
Fragments arranged by size:
817 690 611 189 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 346 2210 2213 2313
Size: 346 1864 3 100
Fragments arranged by size:
1864 346 100 3
BglII A'GATC_T
Cuts at: 0 264 1297 2313
Size: 264 1033 1016
Fragments arranged by size:
1033 1016 264
BlpI GC'TnA_GC
Cuts at: 0 1306 1319 2313
Size: 1306 13 994
Fragments arranged by size:
1306 994 13
BmgBI CAC'GTC
Cuts at: 0 1869 2208 2278 2313
Size: 1869 339 70 35
Fragments arranged by size:
1869 339 70 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 1916 2313
Size: 1916 397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 571 970 994 2313
Size: 502 69 399 24 1319
Fragments arranged by size:
1319 502 399 69 24
Bpu10I CC'TnA_GC
Cuts at: 0 1129 1178 2313
Size: 1129 49 1135
Fragments arranged by size:
1135 1129 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 292 1216 2211 2313
Size: 102 190 924 995 102
Fragments arranged by size:
995 924 190 102 102
BsaI GGTCTCn'nnnn_
Cuts at: 0 1503 2313
Size: 1503 810
BsaHI Gr'CG_yC
Cuts at: 0 1938 2313
Size: 1938 375
BsaWI w'CCGG_w
Cuts at: 0 2216 2313
Size: 2216 97
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 992 1022 1354 1384 1483 1513 1673 1689
Size: 992 30 332 30 99 30 160 16
Cuts at: 1689 1703 1719 2313
Size: 14 16 594
Fragments arranged by size:
992 594 332 160 99 30 30 30
16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 562 1051 1504 1756 1774 2313
Size: 505 57 489 453 252 18 539
Fragments arranged by size:
539 505 489 453 252 57 18
BseYI C'CCAG_C
Cuts at: 0 1548 1798 1889 2313
Size: 1548 250 91 424
Fragments arranged by size:
1548 424 250 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1122 2313
Size: 1114 8 1191
Fragments arranged by size:
1191 1114 8
BsiHKAI G_wGCw'C
Cuts at: 0 524 1307 2313
Size: 524 783 1006
Fragments arranged by size:
1006 783 524
BsmI GAATG_Cn'
Cuts at: 0 397 1107 2313
Size: 397 710 1206
Fragments arranged by size:
1206 710 397
Bsp1286I G_dGCh'C
Cuts at: 0 524 690 696 1307 1496 2313
Size: 524 166 6 611 189 817
Fragments arranged by size:
817 611 524 189 166 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1482 2313
Size: 1482 831
BsrDI GCAATG_nn'
Cuts at: 0 500 2313
Size: 500 1813
BsrFI r'CCGG_y
Cuts at: 0 11 452 1940 2216 2313
Size: 11 441 1488 276 97
Fragments arranged by size:
1488 441 276 97 11
BstXI CCAn_nnnn'nTGG
Cuts at: 0 873 904 2313
Size: 873 31 1409
Fragments arranged by size:
1409 873 31
BstYI r'GATC_y
Cuts at: 0 264 380 1297 2110 2313
Size: 264 116 917 813 203
Fragments arranged by size:
917 813 264 203 116
Bsu36I CC'TnA_GG
Cuts at: 0 1646 1961 2313
Size: 1646 315 352
Fragments arranged by size:
1646 352 315
BtgI C'CryG_G
Cuts at: 0 1203 2313
Size: 1203 1110
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1850 1885 2313
Size: 1850 35 428
Fragments arranged by size:
1850 428 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 439 2242 2313
Size: 439 1803 71
Fragments arranged by size:
1803 439 71
EaeI y'GGCC_r
Cuts at: 0 299 935 2313
Size: 299 636 1378
Fragments arranged by size:
1378 636 299
EarI CTCTTCn'nnn_
Cuts at: 0 362 647 720 1028 1481 1529 1538 1751
Size: 362 285 73 308 453 48 9 213
Cuts at: 1751 1828 2313
Size: 77 485
Fragments arranged by size:
485 453 362 308 285 213 77 73
48 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 189 2313
Size: 189 2124
EcoICRI GAG'CTC
Cuts at: 0 1305 2313
Size: 1305 1008
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 254 466 502 571 664 970 994
Size: 226 28 212 36 69 93 306 24
Cuts at: 994 2313
Size: 1319
Fragments arranged by size:
1319 306 226 212 93 69 36 28
24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 553 652 2313
Size: 553 99 1661
Fragments arranged by size:
1661 553 99
EcoO109I rG'GnC_Cy
Cuts at: 0 432 1173 1619 1966 2313
Size: 432 741 446 347 347
Fragments arranged by size:
741 446 432 347 347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1084 2313
Size: 1084 1229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 111 143 1289 1321 1354 1386 2313
Size: 111 32 1146 32 33 32 927
Fragments arranged by size:
1146 927 111 33 32 32 32
HindIII A'AGCT_T
Cuts at: 0 133 1790 2313
Size: 133 1657 523
Fragments arranged by size:
1657 523 133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2213 2313
Size: 2213 100
MscI TGG'CCA
Cuts at: 0 301 937 2313
Size: 301 636 1376
Fragments arranged by size:
1376 636 301
MspA1I CmG'CkG
Cuts at: 0 468 810 829 2032 2292 2313
Size: 468 342 19 1203 260 21
Fragments arranged by size:
1203 468 342 260 21 19
NcoI C'CATG_G
Cuts at: 0 1203 2313
Size: 1203 1110
NspI r_CATG'y
Cuts at: 0 520 2313
Size: 520 1793
PciI A'CATG_T
Cuts at: 0 516 2313
Size: 516 1797
PfoI T'CCnGG_A
Cuts at: 0 1417 2313
Size: 1417 896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 506 538 2313
Size: 506 32 1775
Fragments arranged by size:
1775 506 32
PpuMI rG'GwC_Cy
Cuts at: 0 432 1173 1619 1966 2313
Size: 432 741 446 347 347
Fragments arranged by size:
741 446 432 347 347
PstI C_TGCA'G
Cuts at: 0 701 834 2313
Size: 701 133 1479
Fragments arranged by size:
1479 701 133
PvuII CAG'CTG
Cuts at: 0 468 810 829 2032 2292 2313
Size: 468 342 19 1203 260 21
Fragments arranged by size:
1203 468 342 260 21 19
SacI G_AGCT'C
Cuts at: 0 1307 2313
Size: 1307 1006
SanDI GG'GwC_CC
Cuts at: 0 432 2313
Size: 432 1881
SapI GCTCTTCn'nnn_
Cuts at: 0 1529 1538 2313
Size: 1529 9 775
Fragments arranged by size:
1529 775 9
ScaI AGT'ACT
Cuts at: 0 1385 2313
Size: 1385 928
SfcI C'TryA_G
Cuts at: 0 697 830 1504 1681 2306 2313
Size: 697 133 674 177 625 7
Fragments arranged by size:
697 674 625 177 133 7
SmlI C'TyrA_G
Cuts at: 0 81 271 889 1195 2226 2313
Size: 81 190 618 306 1031 87
Fragments arranged by size:
1031 618 306 190 87 81
StuI AGG'CCT
Cuts at: 0 628 2313
Size: 628 1685
StyI C'CwwG_G
Cuts at: 0 716 1203 2156 2313
Size: 716 487 953 157
Fragments arranged by size:
953 716 487 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2140 2313
Size: 2140 173
TatI w'GTAC_w
Cuts at: 0 1383 1521 2313
Size: 1383 138 792
Fragments arranged by size:
1383 792 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 992 1024 1689 1721 2313
Size: 992 32 665 32 592
Fragments arranged by size:
992 665 592 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 436 2313
Size: 436 1877
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 945 2313
Size: 945 1368
XmnI GAAnn'nnTTC
Cuts at: 0 1565 2313
Size: 1565 748
Enzymes that do cut:
AcuI AflII AflIII AgeI AlfI AloI AlwNI ApoI
AvaI BamHI BanII BbsI BglII BlpI BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI
BstXI BstYI Bsu36I BtgI CspCI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HindIII
MmeI MscI MspA1I NcoI NspI PciI PfoI PpiI
PpuMI PstI PvuII SacI SanDI SapI ScaI SfcI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AhdI AleI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI Bme1580I
BmtI BplI BsaAI BsaBI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI BtsI ClaI DraI DraIII EagI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI XmaI ZraI