Restriction map for
pFN21AE1531
(Linear) (Six-base) MAPSORT of: KIEE1531.seq Check: 2724 from: 1 to: 1200
KIEE1531.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AatII G_ACGT'C
Cuts at: 0 443 770 902 1200
Size: 443 327 132 298
Fragments arranged by size:
443 327 298 132
Acc65I G'GTAC_C
Cuts at: 0 352 1200
Size: 352 848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 1200
Size: 153 1047
AlwNI CAG_nnn'CTG
Cuts at: 0 324 735 1062 1200
Size: 324 411 327 138
Fragments arranged by size:
411 327 324 138
ApaLI G'TGCA_C
Cuts at: 0 240 1200
Size: 240 960
ApoI r'AATT_y
Cuts at: 0 135 254 336 366 940 1138 1200
Size: 135 119 82 30 574 198 62
Fragments arranged by size:
574 198 135 119 82 62 30
AseI AT'TA_AT
Cuts at: 0 812 1200
Size: 812 388
BanI G'GyrC_C
Cuts at: 0 90 352 1200
Size: 90 262 848
Fragments arranged by size:
848 262 90
BanII G_rGCy'C
Cuts at: 0 863 922 1200
Size: 863 59 278
Fragments arranged by size:
863 278 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 1124 1200
Size: 1124 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1005 1039 1200
Size: 1005 34 161
Fragments arranged by size:
1005 161 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1046 1200
Size: 1046 154
Bme1580I G_kGCm'C
Cuts at: 0 244 1200
Size: 244 956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 945 1200
Size: 108 837 255
Fragments arranged by size:
837 255 108
BsaAI yAC'GTr
Cuts at: 0 291 1200
Size: 291 909
BsaHI Gr'CG_yC
Cuts at: 0 440 767 899 1200
Size: 440 327 132 301
Fragments arranged by size:
440 327 301 132
BsaWI w'CCGG_w
Cuts at: 0 488 1200
Size: 488 712
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 475 505 1200
Size: 475 30 695
Fragments arranged by size:
695 475 30
BseYI C'CCAG_C
Cuts at: 0 45 143 661 785 1200
Size: 45 98 518 124 415
Fragments arranged by size:
518 415 124 98 45
BsiHKAI G_wGCw'C
Cuts at: 0 244 922 989 1200
Size: 244 678 67 211
Fragments arranged by size:
678 244 211 67
BsiWI C'GTAC_G
Cuts at: 0 675 702 1200
Size: 675 27 498
Fragments arranged by size:
675 498 27
BsmI GAATG_Cn'
Cuts at: 0 49 1200
Size: 49 1151
BsmBI CGTCTCn'nnnn_
Cuts at: 0 566 1200
Size: 566 634
Bsp1286I G_dGCh'C
Cuts at: 0 244 863 922 989 1200
Size: 244 619 59 67 211
Fragments arranged by size:
619 244 211 67 59
BsrDI GCAATG_nn'
Cuts at: 0 672 1200
Size: 672 528
BsrGI T'GTAC_A
Cuts at: 0 455 974 1200
Size: 455 519 226
Fragments arranged by size:
519 455 226
BstYI r'GATC_y
Cuts at: 0 75 485 1200
Size: 75 410 715
Fragments arranged by size:
715 410 75
Bsu36I CC'TnA_GG
Cuts at: 0 1048 1200
Size: 1048 152
BtsI GCAGTG_nn'
Cuts at: 0 693 1200
Size: 693 507
DraI TTT'AAA
Cuts at: 0 1038 1200
Size: 1038 162
DraIII CAC_nnn'GTG
Cuts at: 0 879 1200
Size: 879 321
EarI CTCTTCn'nnn_
Cuts at: 0 1166 1200
Size: 1166 34
EcoICRI GAG'CTC
Cuts at: 0 920 1200
Size: 920 280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 153 945 1200
Size: 108 45 792 255
Fragments arranged by size:
792 255 108 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 414 1200
Size: 414 786
EcoO109I rG'GnC_Cy
Cuts at: 0 722 1200
Size: 722 478
EcoRI G'AATT_C
Cuts at: 0 1138 1200
Size: 1138 62
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 198 1200
Size: 198 1002
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 477 509 532 564 1200
Size: 477 32 23 32 636
Fragments arranged by size:
636 477 32 32 23
HincII GTy'rAC
Cuts at: 0 582 1200
Size: 582 618
HpaI GTT'AAC
Cuts at: 0 582 1200
Size: 582 618
KpnI G_GTAC'C
Cuts at: 0 356 1200
Size: 356 844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1200
Size: 448 752
MslI CAynn'nnrTG
Cuts at: 0 982 1200
Size: 982 218
MspA1I CmG'CkG
Cuts at: 0 358 762 1059 1200
Size: 358 404 297 141
Fragments arranged by size:
404 358 297 141
PflMI CCAn_nnn'nTGG
Cuts at: 0 1113 1200
Size: 1113 87
PfoI T'CCnGG_A
Cuts at: 0 124 922 1200
Size: 124 798 278
Fragments arranged by size:
798 278 124
PmlI CAC'GTG
Cuts at: 0 291 1200
Size: 291 909
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 203 235 952 984 1200
Size: 203 32 717 32 216
Fragments arranged by size:
717 216 203 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 722 1200
Size: 722 478
PstI C_TGCA'G
Cuts at: 0 897 1200
Size: 897 303
PvuII CAG'CTG
Cuts at: 0 762 1200
Size: 762 438
SacI G_AGCT'C
Cuts at: 0 922 1200
Size: 922 278
SfcI C'TryA_G
Cuts at: 0 893 1200
Size: 893 307
SpeI A'CTAG_T
Cuts at: 0 298 1200
Size: 298 902
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 59 1200
Size: 59 1141
TatI w'GTAC_w
Cuts at: 0 455 974 1200
Size: 455 519 226
Fragments arranged by size:
519 455 226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 475 507 509 541 1200
Size: 475 32 2 32 659
Fragments arranged by size:
659 475 32 32 2
ZraI GAC'GTC
Cuts at: 0 441 768 900 1200
Size: 441 327 132 300
Fragments arranged by size:
441 327 300 132
Enzymes that do cut:
AatII Acc65I AcuI AlwNI ApaLI ApoI AseI BanI
BanII BbsI BcgI BciVI Bme1580I BpmI BsaAI BsaHI
BsaWI BsaXI BseYI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BsrDI BsrGI BstYI Bsu36I BtsI DraI DraIII EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII
HpaI KpnI MmeI MslI MspA1I PflMI PfoI PmlI
PpiI PpuMI PstI PvuII SacI SfcI SpeI TaqII
TatI TstI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BseRI BsgI BsiEI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgI BtgZI ClaI CspCI DrdI EaeI EagI EciI
EcoRV FseI FspI FspAI HaeII HindIII KasI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI
StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI