Restriction map for
pFN21AE1530
(Linear) (Six-base) MAPSORT of: KIEE1530.seq Check: 5120 from: 1 to: 1338
KIEE1530.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AccI GT'mk_AC
Cuts at: 0 146 1338
Size: 146 1192
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 664 1102 1338
Size: 483 181 438 236
Fragments arranged by size:
483 438 236 181
AleI CACnn'nnGTG
Cuts at: 0 535 1338
Size: 535 803
AlwNI CAG_nnn'CTG
Cuts at: 0 990 1128 1338
Size: 990 138 210
Fragments arranged by size:
990 210 138
ApaI G_GGCC'C
Cuts at: 0 849 959 1338
Size: 849 110 379
Fragments arranged by size:
849 379 110
ApaLI G'TGCA_C
Cuts at: 0 13 565 1338
Size: 13 552 773
Fragments arranged by size:
773 552 13
AvaI C'yCGr_G
Cuts at: 0 726 859 1338
Size: 726 133 479
Fragments arranged by size:
726 479 133
BanI G'GyrC_C
Cuts at: 0 49 211 771 1338
Size: 49 162 560 567
Fragments arranged by size:
567 560 162 49
BanII G_rGCy'C
Cuts at: 0 433 545 849 866 959 1338
Size: 433 112 304 17 93 379
Fragments arranged by size:
433 379 304 112 93 17
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1002 1036 1338
Size: 1002 34 302
Fragments arranged by size:
1002 302 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1266 1338
Size: 1266 72
BclI T'GATC_A
Cuts at: 0 68 1338
Size: 68 1270
BglI GCCn_nnn'nGGC
Cuts at: 0 243 392 1338
Size: 243 149 946
Fragments arranged by size:
946 243 149
Bme1580I G_kGCm'C
Cuts at: 0 17 182 314 445 569 776 849 860
Size: 17 165 132 131 124 207 73 11
Cuts at: 860 959 1338
Size: 99 379
Fragments arranged by size:
379 207 165 132 131 124 99 73
17 11
BmgBI CAC'GTC
Cuts at: 0 354 1338
Size: 354 984
BmrI ACTGGGnnnn_n'
Cuts at: 0 309 1338
Size: 309 1029
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1170 1338
Size: 1170 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1338
Size: 151 1187
Bpu10I CC'TnA_GC
Cuts at: 0 432 1338
Size: 432 906
BsaI GGTCTCn'nnnn_
Cuts at: 0 586 1338
Size: 586 752
BsaAI yAC'GTr
Cuts at: 0 108 1338
Size: 108 1230
BsaHI Gr'CG_yC
Cuts at: 0 884 1338
Size: 884 454
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 92 122 1338
Size: 92 30 1216
Fragments arranged by size:
1216 92 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 484 1338
Size: 484 854
BseYI C'CCAG_C
Cuts at: 0 414 869 1338
Size: 414 455 469
Fragments arranged by size:
469 455 414
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 772 1168 1338
Size: 308 464 396 170
Fragments arranged by size:
464 396 308 170
BsiEI CG_ry'CG
Cuts at: 0 98 1338
Size: 98 1240
BsiHKAI G_wGCw'C
Cuts at: 0 17 83 377 569 866 1338
Size: 17 66 294 192 297 472
Fragments arranged by size:
472 297 294 192 66 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 170 1338
Size: 170 1168
Bsp1286I G_dGCh'C
Cuts at: 0 17 83 182 314 377 433 445 545
Size: 17 66 99 132 63 56 12 100
Cuts at: 545 569 776 849 860 866 959 1338
Size: 24 207 73 11 6 93 379
Fragments arranged by size:
379 207 132 100 99 93 73 66
63 56 24 17 12 11 6
BspHI T'CATG_A
Cuts at: 0 488 1338
Size: 488 850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 755 1338
Size: 755 583
BsrBI CCG'CTC
Cuts at: 0 816 1338
Size: 816 522
BstEII G'GTnAC_C
Cuts at: 0 705 1338
Size: 705 633
BstYI r'GATC_y
Cuts at: 0 461 1338
Size: 461 877
BtgI C'CryG_G
Cuts at: 0 215 825 918 935 951 1074 1338
Size: 215 610 93 17 16 123 264
Fragments arranged by size:
610 264 215 123 93 17 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 89 1338
Size: 89 1249
BtsI GCAGTG_nn'
Cuts at: 0 350 375 1338
Size: 350 25 963
Fragments arranged by size:
963 350 25
ClaI AT'CG_AT
Cuts at: 0 1331 1338
Size: 1331 7
DraIII CAC_nnn'GTG
Cuts at: 0 684 1338
Size: 684 654
EaeI y'GGCC_r
Cuts at: 0 95 276 899 921 938 992 1052 1115
Size: 95 181 623 22 17 54 60 63
Cuts at: 1115 1338
Size: 223
Fragments arranged by size:
623 223 181 95 63 60 54 22
17
EagI C'GGCC_G
Cuts at: 0 95 1338
Size: 95 1243
EarI CTCTTCn'nnn_
Cuts at: 0 1316 1338
Size: 1316 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 678 1246 1338
Size: 678 568 92
Fragments arranged by size:
678 568 92
EcoICRI GAG'CTC
Cuts at: 0 864 1338
Size: 864 474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 483 664 1102 1338
Size: 151 332 181 438 236
Fragments arranged by size:
438 332 236 181 151
EcoO109I rG'GnC_Cy
Cuts at: 0 669 846 1338
Size: 669 177 492
Fragments arranged by size:
669 492 177
FspI TGC'GCA
Cuts at: 0 751 1338
Size: 751 587
HaeII r_GCGC'y
Cuts at: 0 124 619 1338
Size: 124 495 719
Fragments arranged by size:
719 495 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 1240 1272 1338
Size: 148 32 1060 32 66
Fragments arranged by size:
1060 148 66 32 32
HindIII A'AGCT_T
Cuts at: 0 1304 1338
Size: 1304 34
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 1338
Size: 506 832
MscI TGG'CCA
Cuts at: 0 278 940 994 1338
Size: 278 662 54 344
Fragments arranged by size:
662 344 278 54
MslI CAynn'nnrTG
Cuts at: 0 535 964 1338
Size: 535 429 374
Fragments arranged by size:
535 429 374
MspA1I CmG'CkG
Cuts at: 0 129 573 827 953 1076 1338
Size: 129 444 254 126 123 262
Fragments arranged by size:
444 262 254 129 126 123
NcoI C'CATG_G
Cuts at: 0 215 918 1338
Size: 215 703 420
Fragments arranged by size:
703 420 215
PasI CC'CwG_GG
Cuts at: 0 182 1338
Size: 182 1156
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 576 1338
Size: 59 517 762
Fragments arranged by size:
762 517 59
PfoI T'CCnGG_A
Cuts at: 0 476 1121 1338
Size: 476 645 217
Fragments arranged by size:
645 476 217
PpuMI rG'GwC_Cy
Cuts at: 0 669 1338
Size: 669 669
PspOMI G'GGCC_C
Cuts at: 0 845 955 1338
Size: 845 110 383
Fragments arranged by size:
845 383 110
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 1338
Size: 1000 32 306
Fragments arranged by size:
1000 306 32
PstI C_TGCA'G
Cuts at: 0 128 392 1338
Size: 128 264 946
Fragments arranged by size:
946 264 128
PvuII CAG'CTG
Cuts at: 0 129 573 1338
Size: 129 444 765
Fragments arranged by size:
765 444 129
RsrII CG'GwC_CG
Cuts at: 0 480 1338
Size: 480 858
SacI G_AGCT'C
Cuts at: 0 866 1338
Size: 866 472
SacII CC_GC'GG
Cuts at: 0 828 954 1077 1338
Size: 828 126 123 261
Fragments arranged by size:
828 261 126 123
SbfI CC_TGCA'GG
Cuts at: 0 392 1338
Size: 392 946
SexAI A'CCwGG_T
Cuts at: 0 680 1250 1338
Size: 680 570 88
Fragments arranged by size:
680 570 88
SfcI C'TryA_G
Cuts at: 0 124 388 1338
Size: 124 264 950
Fragments arranged by size:
950 264 124
SmaI CCC'GGG
Cuts at: 0 728 1338
Size: 728 610
StuI AGG'CCT
Cuts at: 0 1177 1338
Size: 1177 161
StyI C'CwwG_G
Cuts at: 0 215 518 918 1338
Size: 215 303 400 420
Fragments arranged by size:
420 400 303 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 883 1338
Size: 883 455
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 92 124 670 702 1338
Size: 92 32 546 32 636
Fragments arranged by size:
636 546 92 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 894 972 1338
Size: 189 705 78 366
Fragments arranged by size:
705 366 189 78
XmaI C'CCGG_G
Cuts at: 0 726 1338
Size: 726 612
XmnI GAAnn'nnTTC
Cuts at: 0 496 1338
Size: 496 842
Enzymes that do cut:
AccI AcuI AleI AlwNI ApaI ApaLI AvaI BanI
BanII BcgI BciVI BclI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BstEII BstYI BtgI BtgZI BtsI ClaI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI
HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI
PasI PflMI PfoI PpuMI PspOMI PsrI PstI PvuII
RsrII SacI SacII SbfI SexAI SfcI SmaI StuI
StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BfrBI BglII BlpI
BmtI BpuEI BsaBI BsaWI BsiWI BsmI BspEI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I CspCI DraI DrdI EcoNI EcoRI EcoRV FalI
FseI FspAI HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PvuI SalI SanDI SapI ScaI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XhoI ZraI