(Linear) (Six-base) MAPSORT of: KIEE1529.seq  Check: 7734  from: 1  to: 441



KIEE1529.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:41  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      60     441 
   Size:         60     381
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     441 
   Size:         28     413
 
ApoI r'AATT_y

Cuts at:      0     215     441 
   Size:        215     226
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     138     441 
   Size:        138     303
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      34      68     441 
   Size:         34      34     373

  Fragments arranged by size: 

                 373      34      34
 
BsaBI GATnn'nnATC

Cuts at:      0     338     441 
   Size:        338     103
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      60     441 
   Size:         60     381
 
BsrGI T'GTAC_A

Cuts at:      0     159     441 
   Size:        159     282
 
BstEII G'GTnAC_C

Cuts at:      0     278     441 
   Size:        278     163
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     111     441 
   Size:        111     330
 
DraI TTT'AAA

Cuts at:      0     186     441 
   Size:        186     255
 
EarI CTCTTCn'nnn_

Cuts at:      0       4     441 
   Size:          4     437
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     441 
   Size:         28     413
 
EcoO109I rG'GnC_Cy

Cuts at:      0     115     441 
   Size:        115     326
 
HaeII r_GCGC'y

Cuts at:      0       7     441 
   Size:          7     434
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     162     194     441 
   Size:        162      32     247

  Fragments arranged by size: 

                 247     162      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     441 
   Size:        193     248
 
MslI CAynn'nnrTG

Cuts at:      0     109     441 
   Size:        109     332
 
MspA1I CmG'CkG

Cuts at:      0      67     441 
   Size:         67     374
 
NdeI CA'TA_TG

Cuts at:      0     316     441 
   Size:        316     125
 
NspI r_CATG'y

Cuts at:      0     412     441 
   Size:        412      29
 
PvuII CAG'CTG

Cuts at:      0      67     441 
   Size:         67     374
 
SfcI C'TryA_G

Cuts at:      0      37     441 
   Size:         37     404
 
TatI w'GTAC_w

Cuts at:      0     159     441 
   Size:        159     282
 

 Enzymes that do cut:

     AarI     AcuI     ApoI     BbsI     BcgI    BsaBI    BspMI    BsrGI
   BstEII    BstXI     DraI     EarI  Eco57MI EcoO109I    HaeII    Hin4I
     MmeI     MslI   MspA1I     NdeI     NspI    PvuII     SfcI     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI