(Linear) (Six-base) MAPSORT of: KIEE1528.seq  Check: 8491  from: 1  to: 456



KIEE1528.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:41  ..

AccI GT'mk_AC

Cuts at:      0       4     456 
   Size:          4     452
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     259     456 
   Size:        200      59     197

  Fragments arranged by size: 

                 200     197      59
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     125     456 
   Size:        125     331
 
AlwNI CAG_nnn'CTG

Cuts at:      0     333     456 
   Size:        333     123
 
ApoI r'AATT_y

Cuts at:      0     172     456 
   Size:        172     284
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      33     456 
   Size:         33     423
 
BsmI GAATG_Cn'

Cuts at:      0     114     456 
   Size:        114     342
 
BtsI GCAGTG_nn'

Cuts at:      0     109     456 
   Size:        109     347
 
ClaI AT'CG_AT

Cuts at:      0     282     456 
   Size:        282     174
 
DraI TTT'AAA

Cuts at:      0     159     456 
   Size:        159     297
 
EaeI y'GGCC_r

Cuts at:      0     425     456 
   Size:        425      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     259     456 
   Size:        200      59     197

  Fragments arranged by size: 

                 200     197      59
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54     456 
   Size:         54     402
 
EcoRV GAT'ATC

Cuts at:      0     270     456 
   Size:        270     186
 
HincII GTy'rAC

Cuts at:      0       5     456 
   Size:          5     451
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     456 
   Size:        316     140
 
MslI CAynn'nnrTG

Cuts at:      0     236     456 
   Size:        236     220
 
NdeI CA'TA_TG

Cuts at:      0     259     297     456 
   Size:        259      38     159

  Fragments arranged by size: 

                 259     159      38
 
PpuMI rG'GwC_Cy

Cuts at:      0      54     456 
   Size:         54     402
 
SalI G'TCGA_C

Cuts at:      0       3     456 
   Size:          3     453
 
SpeI A'CTAG_T

Cuts at:      0     162     456 
   Size:        162     294
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      55     456 
   Size:         55     401
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApoI    BseRI     BsmI     BtsI
     ClaI     DraI     EaeI  Eco57MI EcoO109I    EcoRV   HincII     MmeI
     MslI     NdeI    PpuMI     SalI     SpeI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI