Restriction map for
pFN21AE1527
(Linear) (Six-base) MAPSORT of: KIEE1527.seq Check: 2109 from: 1 to: 651
KIEE1527.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AccI GT'mk_AC
Cuts at: 0 404 651
Size: 404 247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 651
Size: 397 254
AflIII A'CryG_T
Cuts at: 0 524 651
Size: 524 127
AvaI C'yCGr_G
Cuts at: 0 185 651
Size: 185 466
BbvCI CC'TCA_GC
Cuts at: 0 589 651
Size: 589 62
BciVI GTATCCnnnnn_n'
Cuts at: 0 450 651
Size: 450 201
BglII A'GATC_T
Cuts at: 0 548 651
Size: 548 103
Bme1580I G_kGCm'C
Cuts at: 0 612 651
Size: 612 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 651
Size: 74 577
Bpu10I CC'TnA_GC
Cuts at: 0 589 651
Size: 589 62
BsaAI yAC'GTr
Cuts at: 0 438 651
Size: 438 213
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 72 102 556 586 651
Size: 72 30 454 30 65
Fragments arranged by size:
454 72 65 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 259 355 385 630 651
Size: 58 201 96 30 245 21
Fragments arranged by size:
245 201 96 58 30 21
BseYI C'CCAG_C
Cuts at: 0 100 211 257 291 651
Size: 100 111 46 34 360
Fragments arranged by size:
360 111 100 46 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 651
Size: 271 380
Bsp1286I G_dGCh'C
Cuts at: 0 612 651
Size: 612 39
BspHI T'CATG_A
Cuts at: 0 75 651
Size: 75 576
BsrBI CCG'CTC
Cuts at: 0 162 651
Size: 162 489
BsrFI r'CCGG_y
Cuts at: 0 53 651
Size: 53 598
BssHII G'CGCG_C
Cuts at: 0 168 651
Size: 168 483
BstAPI GCAn_nnn'nTGC
Cuts at: 0 113 651
Size: 113 538
BstYI r'GATC_y
Cuts at: 0 548 651
Size: 548 103
Bsu36I CC'TnA_GG
Cuts at: 0 242 651
Size: 242 409
EarI CTCTTCn'nnn_
Cuts at: 0 358 651
Size: 358 293
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 64 651
Size: 64 587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 397 651
Size: 74 323 254
Fragments arranged by size:
323 254 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 424 456 651
Size: 70 32 322 32 195
Fragments arranged by size:
322 195 70 32 32
MslI CAynn'nnrTG
Cuts at: 0 559 651
Size: 559 92
MspA1I CmG'CkG
Cuts at: 0 94 215 295 543 651
Size: 94 121 80 248 108
Fragments arranged by size:
248 121 108 94 80
NspI r_CATG'y
Cuts at: 0 351 400 651
Size: 351 49 251
Fragments arranged by size:
351 251 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 399 651
Size: 399 252
PfoI T'CCnGG_A
Cuts at: 0 148 651
Size: 148 503
PstI C_TGCA'G
Cuts at: 0 203 548 623 651
Size: 203 345 75 28
Fragments arranged by size:
345 203 75 28
PvuII CAG'CTG
Cuts at: 0 215 543 651
Size: 215 328 108
Fragments arranged by size:
328 215 108
RsrII CG'GwC_CG
Cuts at: 0 51 651
Size: 51 600
SapI GCTCTTCn'nnn_
Cuts at: 0 358 651
Size: 358 293
SfcI C'TryA_G
Cuts at: 0 138 199 544 619 651
Size: 138 61 345 75 32
Fragments arranged by size:
345 138 75 61 32
SmaI CCC'GGG
Cuts at: 0 187 651
Size: 187 464
SnaBI TAC'GTA
Cuts at: 0 438 651
Size: 438 213
StuI AGG'CCT
Cuts at: 0 181 651
Size: 181 470
StyI C'CwwG_G
Cuts at: 0 455 651
Size: 455 196
TatI w'GTAC_w
Cuts at: 0 332 651
Size: 332 319
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 222 254 290 322 556 588 651
Size: 222 32 36 32 234 32 63
Fragments arranged by size:
234 222 63 36 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 651
Size: 396 255
XmaI C'CCGG_G
Cuts at: 0 185 651
Size: 185 466
Enzymes that do cut:
AccI AcuI AflIII AvaI BbvCI BciVI BglII Bme1580I
BpmI Bpu10I BsaAI BsaXI BseRI BseYI BsgI Bsp1286I
BspHI BsrBI BsrFI BssHII BstAPI BstYI Bsu36I EarI
EciI Eco57MI Hin4I MslI MspA1I NspI PflMI PfoI
PstI PvuII RsrII SapI SfcI SmaI SnaBI StuI
StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI
BbsI BcgI BclI BfrBI BglI BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrDI BsrGI
BssSI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SpeI SphI SrfI SspI
SwaI TaqII Tth111I XbaI XhoI XmnI ZraI