Restriction map for
pFN21AE1526
(Linear) (Six-base) MAPSORT of: KIEE1526.seq Check: 4839 from: 1 to: 1086
KIEE1526.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 458 543 723 1086
Size: 458 85 180 363
Fragments arranged by size:
458 363 180 85
Acc65I G'GTAC_C
Cuts at: 0 464 1086
Size: 464 622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 200 1086
Size: 53 147 886
Fragments arranged by size:
886 147 53
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 444 466 1086
Size: 444 22 620
Fragments arranged by size:
620 444 22
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1009 1041 1086
Size: 1009 32 45
Fragments arranged by size:
1009 45 32
AlwNI CAG_nnn'CTG
Cuts at: 0 231 558 777 837 990 1086
Size: 231 327 219 60 153 96
Fragments arranged by size:
327 231 219 153 96 60
ApaI G_GGCC'C
Cuts at: 0 378 1086
Size: 378 708
AvaI C'yCGr_G
Cuts at: 0 114 1086
Size: 114 972
BanI G'GyrC_C
Cuts at: 0 464 568 1086
Size: 464 104 518
Fragments arranged by size:
518 464 104
BanII G_rGCy'C
Cuts at: 0 115 178 378 894 968 1086
Size: 115 63 200 516 74 118
Fragments arranged by size:
516 200 118 115 74 63
BglI GCCn_nnn'nGGC
Cuts at: 0 247 346 698 1086
Size: 247 99 352 388
Fragments arranged by size:
388 352 247 99
Bme1580I G_kGCm'C
Cuts at: 0 378 573 1086
Size: 378 195 513
Fragments arranged by size:
513 378 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 961 1086
Size: 961 125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 400 490 979 1041 1042 1086
Size: 182 218 90 489 62 1 44
Fragments arranged by size:
489 218 182 90 62 44 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1086
Size: 730 356
BsaAI yAC'GTr
Cuts at: 0 521 882 1086
Size: 521 361 204
Fragments arranged by size:
521 361 204
BsaBI GATnn'nnATC
Cuts at: 0 163 1086
Size: 163 923
BsaHI Gr'CG_yC
Cuts at: 0 222 605 1086
Size: 222 383 481
Fragments arranged by size:
481 383 222
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 125 155 229 259 1009 1039 1086
Size: 125 30 74 30 750 30 47
Fragments arranged by size:
750 125 74 47 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 90 185 229 286 958 976 1018 1030
Size: 90 95 44 57 672 18 42 12
Cuts at: 1030 1086
Size: 56
Fragments arranged by size:
672 95 90 57 56 44 42 18
12
BseYI C'CCAG_C
Cuts at: 0 106 331 558 670 1062 1086
Size: 106 225 227 112 392 24
Fragments arranged by size:
392 227 225 112 106 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 543 757 918 1086
Size: 543 214 161 168
Fragments arranged by size:
543 214 168 161
BsiEI CG_ry'CG
Cuts at: 0 353 1086
Size: 353 733
BsiHKAI G_wGCw'C
Cuts at: 0 817 863 881 968 1086
Size: 817 46 18 87 118
Fragments arranged by size:
817 118 87 46 18
BsmI GAATG_Cn'
Cuts at: 0 760 1086
Size: 760 326
Bsp1286I G_dGCh'C
Cuts at: 0 115 178 378 573 817 863 881 894
Size: 115 63 200 195 244 46 18 13
Cuts at: 894 968 1086
Size: 74 118
Fragments arranged by size:
244 200 195 118 115 74 63 46
18 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 458 543 723 785 1086
Size: 458 85 180 62 301
Fragments arranged by size:
458 301 180 85 62
BsrFI r'CCGG_y
Cuts at: 0 461 1086
Size: 461 625
BssSI C'ACGA_G
Cuts at: 0 874 1086
Size: 874 212
BstAPI GCAn_nnn'nTGC
Cuts at: 0 735 777 927 1086
Size: 735 42 150 159
Fragments arranged by size:
735 159 150 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 439 825 1086
Size: 439 386 261
Fragments arranged by size:
439 386 261
BtgI C'CryG_G
Cuts at: 0 170 818 1086
Size: 170 648 268
Fragments arranged by size:
648 268 170
BtsI GCAGTG_nn'
Cuts at: 0 326 1086
Size: 326 760
EaeI y'GGCC_r
Cuts at: 0 161 350 389 1086
Size: 161 189 39 697
Fragments arranged by size:
697 189 161 39
EagI C'GGCC_G
Cuts at: 0 350 1086
Size: 350 736
EarI CTCTTCn'nnn_
Cuts at: 0 72 176 283 640 1086
Size: 72 104 107 357 446
Fragments arranged by size:
446 357 107 104 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 679 961 1086
Size: 679 282 125
Fragments arranged by size:
679 282 125
EcoICRI GAG'CTC
Cuts at: 0 966 1086
Size: 966 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 182 200 400 490 979 1041 1042
Size: 53 129 18 200 90 489 62 1
Cuts at: 1042 1086
Size: 44
Fragments arranged by size:
489 200 129 90 62 53 44 18
1
EcoO109I rG'GnC_Cy
Cuts at: 0 374 375 630 806 1086
Size: 374 1 255 176 280
Fragments arranged by size:
374 280 255 176 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 651 1086
Size: 651 435
HaeII r_GCGC'y
Cuts at: 0 43 1086
Size: 43 1043
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 229 261 688 720 1086
Size: 30 199 32 427 32 366
Fragments arranged by size:
427 366 199 32 32 30
KpnI G_GTAC'C
Cuts at: 0 468 1086
Size: 468 618
MscI TGG'CCA
Cuts at: 0 163 1086
Size: 163 923
MslI CAynn'nnrTG
Cuts at: 0 823 1086
Size: 823 263
MspA1I CmG'CkG
Cuts at: 0 172 267 435 837 980 987 1066 1086
Size: 172 95 168 402 143 7 79 20
Fragments arranged by size:
402 172 168 143 95 79 20 7
NcoI C'CATG_G
Cuts at: 0 818 1086
Size: 818 268
NspI r_CATG'y
Cuts at: 0 447 1086
Size: 447 639
PasI CC'CwG_GG
Cuts at: 0 57 1086
Size: 57 1029
PflMI CCAn_nnn'nTGG
Cuts at: 0 837 1086
Size: 837 249
PfoI T'CCnGG_A
Cuts at: 0 545 1086
Size: 545 541
PmlI CAC'GTG
Cuts at: 0 521 882 1086
Size: 521 361 204
Fragments arranged by size:
521 361 204
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1009 1041 1086
Size: 1009 32 45
Fragments arranged by size:
1009 45 32
PpuMI rG'GwC_Cy
Cuts at: 0 806 1086
Size: 806 280
PspOMI G'GGCC_C
Cuts at: 0 374 1086
Size: 374 712
PstI C_TGCA'G
Cuts at: 0 158 236 683 773 995 1086
Size: 158 78 447 90 222 91
Fragments arranged by size:
447 222 158 91 90 78
PvuII CAG'CTG
Cuts at: 0 435 837 987 1086
Size: 435 402 150 99
Fragments arranged by size:
435 402 150 99
SacI G_AGCT'C
Cuts at: 0 968 1086
Size: 968 118
SacII CC_GC'GG
Cuts at: 0 173 1086
Size: 173 913
SanDI GG'GwC_CC
Cuts at: 0 806 1086
Size: 806 280
SapI GCTCTTCn'nnn_
Cuts at: 0 72 176 283 1086
Size: 72 104 107 803
Fragments arranged by size:
803 107 104 72
SfcI C'TryA_G
Cuts at: 0 154 232 679 769 991 1042 1086
Size: 154 78 447 90 222 51 44
Fragments arranged by size:
447 222 154 90 78 51 44
SmlI C'TyrA_G
Cuts at: 0 15 745 1086
Size: 15 730 341
Fragments arranged by size:
730 341 15
SphI G_CATG'C
Cuts at: 0 447 1086
Size: 447 639
StyI C'CwwG_G
Cuts at: 0 144 818 1086
Size: 144 674 268
Fragments arranged by size:
674 268 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 556 1086
Size: 556 530
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 125 157 1086
Size: 125 32 929
Fragments arranged by size:
929 125 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 648 1086
Size: 648 438
Enzymes that do cut:
AarI Acc65I AcuI AlfI AloI AlwNI ApaI AvaI
BanI BanII BglI Bme1580I BmrI BpmI BpuEI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrFI BssSI BstAPI BstXI BtgI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I KpnI MscI MslI MspA1I NcoI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI
PstI PvuII SacI SacII SanDI SapI SfcI SmlI
SphI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaWI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI