Restriction map for
pFN21AE1524
(Linear) (Six-base) MAPSORT of: KIEE1524.seq Check: 4674 from: 1 to: 1983
KIEE1524.orf
With 182 enzymes: SgfI *
July 15, 2010 18:41 ..
AccI GT'mk_AC
Cuts at: 0 227 1983
Size: 227 1756
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 1983
Size: 52 1931
AflIII A'CryG_T
Cuts at: 0 534 914 1983
Size: 534 380 1069
Fragments arranged by size:
1069 534 380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 769 1983
Size: 769 1214
AlwNI CAG_nnn'CTG
Cuts at: 0 436 573 1005 1983
Size: 436 137 432 978
Fragments arranged by size:
978 436 432 137
ApaI G_GGCC'C
Cuts at: 0 714 845 1053 1083 1983
Size: 714 131 208 30 900
Fragments arranged by size:
900 714 208 131 30
ApaLI G'TGCA_C
Cuts at: 0 388 724 1983
Size: 388 336 1259
Fragments arranged by size:
1259 388 336
ApoI r'AATT_y
Cuts at: 0 1550 1983
Size: 1550 433
AvaI C'yCGr_G
Cuts at: 0 818 1364 1983
Size: 818 546 619
Fragments arranged by size:
818 619 546
AvrII C'CTAG_G
Cuts at: 0 1027 1983
Size: 1027 956
BanI G'GyrC_C
Cuts at: 0 293 594 814 1183 1354 1983
Size: 293 301 220 369 171 629
Fragments arranged by size:
629 369 301 293 220 171
BanII G_rGCy'C
Cuts at: 0 269 607 714 845 1020 1053 1083 1710
Size: 269 338 107 131 175 33 30 627
Cuts at: 1710 1983
Size: 273
Fragments arranged by size:
627 338 273 269 175 131 107 33
30
BbeI G_GCGC'C
Cuts at: 0 598 1187 1983
Size: 598 589 796
Fragments arranged by size:
796 598 589
BbvCI CC'TCA_GC
Cuts at: 0 1625 1983
Size: 1625 358
BglI GCCn_nnn'nGGC
Cuts at: 0 473 1983
Size: 473 1510
Bme1580I G_kGCm'C
Cuts at: 0 392 478 714 728 819 845 873 1053
Size: 392 86 236 14 91 26 28 180
Cuts at: 1053 1083 1574 1983
Size: 30 491 409
Fragments arranged by size:
491 409 392 236 180 91 86 30
28 26 14
BmgBI CAC'GTC
Cuts at: 0 915 1983
Size: 915 1068
BmrI ACTGGGnnnn_n'
Cuts at: 0 54 231 407 424 493 571 610 1844
Size: 54 177 176 17 69 78 39 1234
Cuts at: 1844 1983
Size: 139
Fragments arranged by size:
1234 177 176 139 78 69 54 39
17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 33 966 1983
Size: 33 933 1017
Fragments arranged by size:
1017 933 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1029 1138 1483 1513 1568 1724 1751
Size: 1027 2 109 345 30 55 156 27
Cuts at: 1751 1948 1983
Size: 197 35
Fragments arranged by size:
1027 345 197 156 109 55 35 30
27 2
Bpu10I CC'TnA_GC
Cuts at: 0 254 302 1625 1983
Size: 254 48 1323 358
Fragments arranged by size:
1323 358 254 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 304 1852 1983
Size: 100 204 1548 131
Fragments arranged by size:
1548 204 131 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 725 1983
Size: 725 1258
BsaAI yAC'GTr
Cuts at: 0 537 1983
Size: 537 1446
BsaHI Gr'CG_yC
Cuts at: 0 595 760 1184 1983
Size: 595 165 424 799
Fragments arranged by size:
799 595 424 165
BsaWI w'CCGG_w
Cuts at: 0 1859 1983
Size: 1859 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 281 311 1832 1862 1983
Size: 281 30 1521 30 121
Fragments arranged by size:
1521 281 121 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 1461 1613 1983
Size: 97 1364 152 370
Fragments arranged by size:
1364 370 152 97
BseYI C'CCAG_C
Cuts at: 0 164 363 1076 1290 1431 1630 1983
Size: 164 199 713 214 141 199 353
Fragments arranged by size:
713 353 214 199 199 164 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 595 1983
Size: 532 63 1388
Fragments arranged by size:
1388 532 63
BsiEI CG_ry'CG
Cuts at: 0 1098 1983
Size: 1098 885
BsiHKAI G_wGCw'C
Cuts at: 0 110 260 392 530 728 1983
Size: 110 150 132 138 198 1255
Fragments arranged by size:
1255 198 150 138 132 110
BsmI GAATG_Cn'
Cuts at: 0 1703 1983
Size: 1703 280
Bsp1286I G_dGCh'C
Cuts at: 0 110 260 269 392 478 530 607 714
Size: 110 150 9 123 86 52 77 107
Cuts at: 714 728 819 845 873 1020 1053 1083 1574
Size: 14 91 26 28 147 33 30 491
Cuts at: 1574 1710 1983
Size: 136 273
Fragments arranged by size:
491 273 150 147 136 123 110 107
91 86 77 52 33 30 28 26
14 9
BspEI T'CCGG_A
Cuts at: 0 1859 1983
Size: 1859 124
BspHI T'CATG_A
Cuts at: 0 512 1983
Size: 512 1471
BsrFI r'CCGG_y
Cuts at: 0 155 1097 1898 1983
Size: 155 942 801 85
Fragments arranged by size:
942 801 155 85
BssHII G'CGCG_C
Cuts at: 0 616 1983
Size: 616 1367
BssSI C'ACGA_G
Cuts at: 0 652 1983
Size: 652 1331
BstEII G'GTnAC_C
Cuts at: 0 1204 1797 1983
Size: 1204 593 186
Fragments arranged by size:
1204 593 186
BstXI CCAn_nnnn'nTGG
Cuts at: 0 780 1491 1983
Size: 780 711 492
Fragments arranged by size:
780 711 492
BstYI r'GATC_y
Cuts at: 0 233 1983
Size: 233 1750
Bsu36I CC'TnA_GG
Cuts at: 0 8 128 1189 1983
Size: 8 120 1061 794
Fragments arranged by size:
1061 794 120 8
BtgI C'CryG_G
Cuts at: 0 289 695 714 888 1691 1983
Size: 289 406 19 174 803 292
Fragments arranged by size:
803 406 292 289 174 19
BtsI GCAGTG_nn'
Cuts at: 0 1307 1983
Size: 1307 676
EaeI y'GGCC_r
Cuts at: 0 100 1973 1983
Size: 100 1873 10
Fragments arranged by size:
1873 100 10
EarI CTCTTCn'nnn_
Cuts at: 0 676 1888 1983
Size: 676 1212 95
Fragments arranged by size:
1212 676 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 1027 1029 1138 1483 1513 1568 1724
Size: 52 975 2 109 345 30 55 156
Cuts at: 1724 1751 1948 1983
Size: 27 197 35
Fragments arranged by size:
975 345 197 156 109 55 52 35
30 27 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 499 1983
Size: 499 1484
EcoO109I rG'GnC_Cy
Cuts at: 0 132 315 457 710 842 978 1049 1050
Size: 132 183 142 253 132 136 71 1
Cuts at: 1050 1080 1579 1944 1983
Size: 30 499 365 39
Fragments arranged by size:
499 365 253 183 142 136 132 132
71 39 30 1
FseI GG_CCGG'CC
Cuts at: 0 159 1983
Size: 159 1824
HaeII r_GCGC'y
Cuts at: 0 598 1187 1983
Size: 598 589 796
Fragments arranged by size:
796 598 589
KasI G'GCGC_C
Cuts at: 0 594 1183 1983
Size: 594 589 800
Fragments arranged by size:
800 594 589
MscI TGG'CCA
Cuts at: 0 1975 1983
Size: 1975 8
MslI CAynn'nnrTG
Cuts at: 0 148 778 1696 1983
Size: 148 630 918 287
Fragments arranged by size:
918 630 287 148
MspA1I CmG'CkG
Cuts at: 0 648 684 716 1002 1720 1954 1983
Size: 648 36 32 286 718 234 29
Fragments arranged by size:
718 648 286 234 36 32 29
NaeI GCC'GGC
Cuts at: 0 157 1983
Size: 157 1826
NarI GG'CG_CC
Cuts at: 0 595 1184 1983
Size: 595 589 799
Fragments arranged by size:
799 595 589
NcoI C'CATG_G
Cuts at: 0 695 1691 1983
Size: 695 996 292
Fragments arranged by size:
996 695 292
NgoMIV G'CCGG_C
Cuts at: 0 155 1983
Size: 155 1828
NspI r_CATG'y
Cuts at: 0 1157 1458 1983
Size: 1157 301 525
Fragments arranged by size:
1157 525 301
PasI CC'CwG_GG
Cuts at: 0 968 1575 1983
Size: 968 607 408
Fragments arranged by size:
968 607 408
PflMI CCAn_nnn'nTGG
Cuts at: 0 1247 1983
Size: 1247 736
PfoI T'CCnGG_A
Cuts at: 0 41 1483 1925 1983
Size: 41 1442 442 58
Fragments arranged by size:
1442 442 58 41
PmlI CAC'GTG
Cuts at: 0 537 1983
Size: 537 1446
PpuMI rG'GwC_Cy
Cuts at: 0 132 457 978 1579 1944 1983
Size: 132 325 521 601 365 39
Fragments arranged by size:
601 521 365 325 132 39
PspOMI G'GGCC_C
Cuts at: 0 710 841 1049 1079 1983
Size: 710 131 208 30 904
Fragments arranged by size:
904 710 208 131 30
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 211 243 1983
Size: 211 32 1740
Fragments arranged by size:
1740 211 32
PstI C_TGCA'G
Cuts at: 0 578 1001 1983
Size: 578 423 982
Fragments arranged by size:
982 578 423
PvuII CAG'CTG
Cuts at: 0 684 1954 1983
Size: 684 1270 29
Fragments arranged by size:
1270 684 29
SacII CC_GC'GG
Cuts at: 0 717 1983
Size: 717 1266
SanDI GG'GwC_CC
Cuts at: 0 978 1983
Size: 978 1005
SapI GCTCTTCn'nnn_
Cuts at: 0 1888 1983
Size: 1888 95
SfcI C'TryA_G
Cuts at: 0 574 997 1983
Size: 574 423 986
Fragments arranged by size:
986 574 423
SfoI GGC'GCC
Cuts at: 0 596 1185 1983
Size: 596 589 798
Fragments arranged by size:
798 596 589
SmaI CCC'GGG
Cuts at: 0 820 1366 1983
Size: 820 546 617
Fragments arranged by size:
820 617 546
SmlI C'TyrA_G
Cuts at: 0 79 319 1867 1983
Size: 79 240 1548 116
Fragments arranged by size:
1548 240 116 79
StuI AGG'CCT
Cuts at: 0 1032 1589 1736 1983
Size: 1032 557 147 247
Fragments arranged by size:
1032 557 247 147
StyI C'CwwG_G
Cuts at: 0 237 695 1027 1691 1983
Size: 237 458 332 664 292
Fragments arranged by size:
664 458 332 292 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 498 545 1394 1501 1816 1983
Size: 498 47 849 107 315 167
Fragments arranged by size:
849 498 315 167 107 47
TatI w'GTAC_w
Cuts at: 0 558 1983
Size: 558 1425
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 281 313 1983
Size: 281 32 1670
Fragments arranged by size:
1670 281 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 702 1983
Size: 702 1281
XmaI C'CCGG_G
Cuts at: 0 818 1364 1983
Size: 818 546 619
Fragments arranged by size:
818 619 546
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI
AvaI AvrII BanI BanII BbeI BbvCI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BsrFI BssHII BssSI BstEII
BstXI BstYI Bsu36I BtgI BtsI EaeI EarI Eco57MI
EcoNI EcoO109I FseI HaeII KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PmlI PpuMI PspOMI PsrI PstI PvuII SacII SanDI
SapI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AscI AseI AsiSI BaeI BamHI BbsI
BcgI BciVI BclI BfrBI BglII BlpI BmtI BsaBI
BsiWI BsmBI BspMI BsrBI BsrDI BsrGI BstAPI BstBI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoRI EcoRV FalI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PpiI
PshAI PsiI PspXI PvuI RsrII SacI SalI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI