(Linear) (Six-base) MAPSORT of: KIEE1523.seq  Check: 6632  from: 1  to: 1110



KIEE1523.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:40  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     349    1110 
   Size:         34     315     761

  Fragments arranged by size: 

                 761     315      34
 
ApoI r'AATT_y

Cuts at:      0     532    1057    1110 
   Size:        532     525      53

  Fragments arranged by size: 

                 532     525      53
 
BanII G_rGCy'C

Cuts at:      0     894    1110 
   Size:        894     216
 
BglII A'GATC_T

Cuts at:      0     853    1110 
   Size:        853     257
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     754    1110 
   Size:        754     356
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     652    1110 
   Size:        652     458
 
BsaWI w'CCGG_w

Cuts at:      0      98    1110 
   Size:         98    1012
 
BseYI C'CCAG_C

Cuts at:      0     823    1110 
   Size:        823     287
 
BsiHKAI G_wGCw'C

Cuts at:      0     139     894    1110 
   Size:        139     755     216

  Fragments arranged by size: 

                 755     216     139
 
BsmI GAATG_Cn'

Cuts at:      0      94    1110 
   Size:         94    1016
 
Bsp1286I G_dGCh'C

Cuts at:      0     139     894    1110 
   Size:        139     755     216

  Fragments arranged by size: 

                 755     216     139
 
BspHI T'CATG_A

Cuts at:      0     251    1110 
   Size:        251     859
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     949    1110 
   Size:        949     161
 
BstYI r'GATC_y

Cuts at:      0     362     477     853    1110 
   Size:        362     115     376     257

  Fragments arranged by size: 

                 376     362     257     115
 
BtgI C'CryG_G

Cuts at:      0      83     778    1110 
   Size:         83     695     332

  Fragments arranged by size: 

                 695     332      83
 
BtsI GCAGTG_nn'

Cuts at:      0     950    1110 
   Size:        950     160
 
EaeI y'GGCC_r

Cuts at:      0     555    1110 
   Size:        555     555
 
EarI CTCTTCn'nnn_

Cuts at:      0     800    1110 
   Size:        800     310
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     355    1110 
   Size:        355     755
 
EcoICRI GAG'CTC

Cuts at:      0     892    1110 
   Size:        892     218
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     349     754    1110 
   Size:         34     315     405     356

  Fragments arranged by size: 

                 405     356     315      34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     373    1110 
   Size:        373     737
 
EcoRI G'AATT_C

Cuts at:      0    1057    1110 
   Size:       1057      53
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      39    1110 
   Size:         39    1071
 
HindIII A'AGCT_T

Cuts at:      0     967    1110 
   Size:        967     143
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1086    1110 
   Size:       1086      24
 
MslI CAynn'nnrTG

Cuts at:      0     250     943    1110 
   Size:        250     693     167

  Fragments arranged by size: 

                 693     250     167
 
NcoI C'CATG_G

Cuts at:      0      83     778    1110 
   Size:         83     695     332

  Fragments arranged by size: 

                 695     332      83
 
NdeI CA'TA_TG

Cuts at:      0     984    1110 
   Size:        984     126
 
NspI r_CATG'y

Cuts at:      0      38      82     705    1110 
   Size:         38      44     623     405

  Fragments arranged by size: 

                 623     405      44      38
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     678    1110 
   Size:        678     432
 
PsiI TTA'TAA

Cuts at:      0     692    1110 
   Size:        692     418
 
SacI G_AGCT'C

Cuts at:      0     894    1110 
   Size:        894     216
 
SfcI C'TryA_G

Cuts at:      0     596     621     653     857    1110 
   Size:        596      25      32     204     253

  Fragments arranged by size: 

                 596     253     204      32      25
 
SphI G_CATG'C

Cuts at:      0      82    1110 
   Size:         82    1028
 
SspI AAT'ATT

Cuts at:      0     215    1110 
   Size:        215     895
 
StyI C'CwwG_G

Cuts at:      0      83     778    1110 
   Size:         83     695     332

  Fragments arranged by size: 

                 695     332      83
 
TatI w'GTAC_w

Cuts at:      0     567    1110 
   Size:        567     543
 

 Enzymes that do cut:

     AcuI     ApoI    BanII    BglII     BpmI     BsaI    BsaWI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspHI   BstAPI    BstYI     BtgI     BtsI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI     FalI
  HindIII     MmeI     MslI     NcoI     NdeI     NspI    PflMI     PsiI
     SacI     SfcI     SphI     SspI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI EcoO109I    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI