Restriction map for
pFN21AE1521
(Linear) (Six-base) MAPSORT of: KIEE1521.seq Check: 9252 from: 1 to: 987
KIEE1521.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AccI GT'mk_AC
Cuts at: 0 203 987
Size: 203 784
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 283 390 987
Size: 97 186 107 597
Fragments arranged by size:
597 186 107 97
AflIII A'CryG_T
Cuts at: 0 979 987
Size: 979 8
ApaLI G'TGCA_C
Cuts at: 0 12 987
Size: 12 975
ApoI r'AATT_y
Cuts at: 0 220 378 459 528 987
Size: 220 158 81 69 459
Fragments arranged by size:
459 220 158 81 69
BamHI G'GATC_C
Cuts at: 0 3 987
Size: 3 984
BbsI GAAGACnn'nnnn_
Cuts at: 0 958 987
Size: 958 29
BbvCI CC'TCA_GC
Cuts at: 0 716 987
Size: 716 271
BglII A'GATC_T
Cuts at: 0 916 987
Size: 916 71
Bme1580I G_kGCm'C
Cuts at: 0 16 987
Size: 16 971
BmrI ACTGGGnnnn_n'
Cuts at: 0 3 987
Size: 3 984
Bpu10I CC'TnA_GC
Cuts at: 0 716 987
Size: 716 271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 727 987
Size: 551 176 260
Fragments arranged by size:
551 260 176
BsaBI GATnn'nnATC
Cuts at: 0 364 987
Size: 364 623
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 62 987
Size: 62 925
BseYI C'CCAG_C
Cuts at: 0 929 987
Size: 929 58
BsiHKAI G_wGCw'C
Cuts at: 0 16 987
Size: 16 971
Bsp1286I G_dGCh'C
Cuts at: 0 16 987
Size: 16 971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 119 475 987
Size: 119 356 512
Fragments arranged by size:
512 356 119
BsrGI T'GTAC_A
Cuts at: 0 452 987
Size: 452 535
BstBI TT'CG_AA
Cuts at: 0 949 987
Size: 949 38
BstEII G'GTnAC_C
Cuts at: 0 484 987
Size: 484 503
BstXI CCAn_nnnn'nTGG
Cuts at: 0 246 858 987
Size: 246 612 129
Fragments arranged by size:
612 246 129
BstYI r'GATC_y
Cuts at: 0 3 916 987
Size: 3 913 71
Fragments arranged by size:
913 71 3
BstZ17I GTA'TAC
Cuts at: 0 204 987
Size: 204 783
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 721 756 987
Size: 721 35 231
Fragments arranged by size:
721 231 35
DraI TTT'AAA
Cuts at: 0 942 987
Size: 942 45
EarI CTCTTCn'nnn_
Cuts at: 0 863 987
Size: 863 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 283 390 987
Size: 97 186 107 597
Fragments arranged by size:
597 186 107 97
EcoO109I rG'GnC_Cy
Cuts at: 0 711 987
Size: 711 276
EcoRI G'AATT_C
Cuts at: 0 378 528 987
Size: 378 150 459
Fragments arranged by size:
459 378 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 884 916 987
Size: 884 32 71
Fragments arranged by size:
884 71 32
HincII GTy'rAC
Cuts at: 0 412 987
Size: 412 575
HindIII A'AGCT_T
Cuts at: 0 86 987
Size: 86 901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 784 987
Size: 256 528 203
Fragments arranged by size:
528 256 203
MslI CAynn'nnrTG
Cuts at: 0 355 960 987
Size: 355 605 27
Fragments arranged by size:
605 355 27
MspA1I CmG'CkG
Cuts at: 0 751 987
Size: 751 236
NspI r_CATG'y
Cuts at: 0 983 987
Size: 983 4
PciI A'CATG_T
Cuts at: 0 979 987
Size: 979 8
PpuMI rG'GwC_Cy
Cuts at: 0 711 987
Size: 711 276
PsiI TTA'TAA
Cuts at: 0 839 987
Size: 839 148
PstI C_TGCA'G
Cuts at: 0 108 987
Size: 108 879
PvuII CAG'CTG
Cuts at: 0 751 987
Size: 751 236
SfcI C'TryA_G
Cuts at: 0 104 698 987
Size: 104 594 289
Fragments arranged by size:
594 289 104
SmlI C'TyrA_G
Cuts at: 0 566 706 987
Size: 566 140 281
Fragments arranged by size:
566 281 140
StyI C'CwwG_G
Cuts at: 0 739 987
Size: 739 248
TatI w'GTAC_w
Cuts at: 0 452 753 987
Size: 452 301 234
Fragments arranged by size:
452 301 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 987
Size: 27 960
Enzymes that do cut:
AccI AcuI AflIII ApaLI ApoI BamHI BbsI BbvCI
BglII Bme1580I BmrI Bpu10I BpuEI BsaBI BseRI BseYI
BsiHKAI Bsp1286I BspMI BsrGI BstBI BstEII BstXI BstYI
BstZ17I CspCI DraI EarI Eco57MI EcoO109I EcoRI Hin4I
HincII HindIII MmeI MslI MspA1I NspI PciI PpuMI
PsiI PstI PvuII SfcI SmlI StyI TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BanI BanII BbeI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmtI BplI BpmI
BsaI BsaAI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI Bsu36I BtgI BtgZI BtsI ClaI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI