Restriction map for
pFN21AE1519
(Linear) (Six-base) MAPSORT of: KIEE1519.seq Check: 8496 from: 1 to: 1392
KIEE1519.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 905 1392
Size: 905 487
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 565 597 1392
Size: 565 32 795
Fragments arranged by size:
795 565 32
AlwNI CAG_nnn'CTG
Cuts at: 0 504 805 916 937 958 1000 1063 1392
Size: 504 301 111 21 21 42 63 329
Fragments arranged by size:
504 329 301 111 63 42 21 21
ApaI G_GGCC'C
Cuts at: 0 821 1392
Size: 821 571
ApaLI G'TGCA_C
Cuts at: 0 1135 1177 1303 1392
Size: 1135 42 126 89
Fragments arranged by size:
1135 126 89 42
AvaI C'yCGr_G
Cuts at: 0 19 342 822 1021 1128 1212 1234 1392
Size: 19 323 480 199 107 84 22 158
Fragments arranged by size:
480 323 199 158 107 84 22 19
BanI G'GyrC_C
Cuts at: 0 546 1392
Size: 546 846
BanII G_rGCy'C
Cuts at: 0 157 524 821 1392
Size: 157 367 297 571
Fragments arranged by size:
571 367 297 157
BbvCI CC'TCA_GC
Cuts at: 0 1268 1333 1392
Size: 1268 65 59
Fragments arranged by size:
1268 65 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 517 543 551 577 1392
Size: 517 26 8 26 815
Fragments arranged by size:
815 517 26 26 8
BglI GCCn_nnn'nGGC
Cuts at: 0 23 265 274 344 729 816 1392
Size: 23 242 9 70 385 87 576
Fragments arranged by size:
576 385 242 87 70 23 9
Bme1580I G_kGCm'C
Cuts at: 0 821 1139 1181 1307 1392
Size: 821 318 42 126 85
Fragments arranged by size:
821 318 126 85 42
BmgBI CAC'GTC
Cuts at: 0 442 1392
Size: 442 950
BmrI ACTGGGnnnn_n'
Cuts at: 0 868 1392
Size: 868 524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 607 836 998 1022 1392
Size: 124 483 229 162 24 370
Fragments arranged by size:
483 370 229 162 124 24
Bpu10I CC'TnA_GC
Cuts at: 0 1268 1333 1392
Size: 1268 65 59
Fragments arranged by size:
1268 65 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 100 523 598 1392
Size: 100 423 75 794
Fragments arranged by size:
794 423 100 75
BsaHI Gr'CG_yC
Cuts at: 0 920 941 1392
Size: 920 21 451
Fragments arranged by size:
920 451 21
BsaWI w'CCGG_w
Cuts at: 0 483 1392
Size: 483 909
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 565 595 650 680 1392
Size: 139 30 396 30 55 30 712
Fragments arranged by size:
712 396 139 55 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 42 45 385 1392
Size: 42 3 340 1007
Fragments arranged by size:
1007 340 42 3
BseYI C'CCAG_C
Cuts at: 0 284 661 833 909 930 951 993 1140
Size: 284 377 172 76 21 21 42 147
Cuts at: 1140 1392
Size: 252
Fragments arranged by size:
377 284 252 172 147 76 42 21
21
BsiEI CG_ry'CG
Cuts at: 0 339 1392
Size: 339 1053
BsiHKAI G_wGCw'C
Cuts at: 0 1139 1181 1307 1392
Size: 1139 42 126 85
Fragments arranged by size:
1139 126 85 42
BsiWI C'GTAC_G
Cuts at: 0 461 1392
Size: 461 931
BsmI GAATG_Cn'
Cuts at: 0 169 1392
Size: 169 1223
Bsp1286I G_dGCh'C
Cuts at: 0 157 524 821 1139 1181 1307 1392
Size: 157 367 297 318 42 126 85
Fragments arranged by size:
367 318 297 157 126 85 42
BspEI T'CCGG_A
Cuts at: 0 483 1392
Size: 483 909
BspHI T'CATG_A
Cuts at: 0 137 1392
Size: 137 1255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 512 1392
Size: 512 880
BsrBI CCG'CTC
Cuts at: 0 38 1392
Size: 38 1354
BsrDI GCAATG_nn'
Cuts at: 0 533 1392
Size: 533 859
BsrFI r'CCGG_y
Cuts at: 0 488 1392
Size: 488 904
BstAPI GCAn_nnn'nTGC
Cuts at: 0 173 504 1392
Size: 173 331 888
Fragments arranged by size:
888 331 173
Bsu36I CC'TnA_GG
Cuts at: 0 813 1163 1205 1392
Size: 813 350 42 187
Fragments arranged by size:
813 350 187 42
EaeI y'GGCC_r
Cuts at: 0 336 512 730 1392
Size: 336 176 218 662
Fragments arranged by size:
662 336 218 176
EagI C'GGCC_G
Cuts at: 0 336 1392
Size: 336 1056
EarI CTCTTCn'nnn_
Cuts at: 0 403 1392
Size: 403 989
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 586 1392
Size: 586 806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 607 836 998 1022 1392
Size: 124 483 229 162 24 370
Fragments arranged by size:
483 370 229 162 124 24
EcoO109I rG'GnC_Cy
Cuts at: 0 126 276 802 817 818 1125 1167 1209
Size: 126 150 526 15 1 307 42 42
Cuts at: 1209 1337 1392
Size: 128 55
Fragments arranged by size:
526 307 150 128 126 55 42 42
15 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 633 1392
Size: 633 759
HaeII r_GCGC'y
Cuts at: 0 87 495 1392
Size: 87 408 897
Fragments arranged by size:
897 408 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 465 497 702 734 1188 1220 1392
Size: 465 32 205 32 454 32 172
Fragments arranged by size:
465 454 205 172 32 32 32
MslI CAynn'nnrTG
Cuts at: 0 191 1392
Size: 191 1201
MspA1I CmG'CkG
Cuts at: 0 96 708 738 1108 1392
Size: 96 612 30 370 284
Fragments arranged by size:
612 370 284 96 30
PasI CC'CwG_GG
Cuts at: 0 682 958 1063 1084 1171 1192 1360 1392
Size: 682 276 105 21 87 21 168 32
Fragments arranged by size:
682 276 168 105 87 32 21 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 916 937 958 979 1000 1063 1392
Size: 916 21 21 21 21 63 329
Fragments arranged by size:
916 329 63 21 21 21 21
PfoI T'CCnGG_A
Cuts at: 0 999 1275 1392
Size: 999 276 117
Fragments arranged by size:
999 276 117
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 565 597 1392
Size: 565 32 795
Fragments arranged by size:
795 565 32
PpuMI rG'GwC_Cy
Cuts at: 0 126 1392
Size: 126 1266
PspOMI G'GGCC_C
Cuts at: 0 817 1392
Size: 817 575
PvuII CAG'CTG
Cuts at: 0 96 1392
Size: 96 1296
RsrII CG'GwC_CG
Cuts at: 0 486 1392
Size: 486 906
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 274 1392
Size: 274 1118
SmaI CCC'GGG
Cuts at: 0 21 344 824 1023 1130 1214 1236 1392
Size: 21 323 480 199 107 84 22 156
Fragments arranged by size:
480 323 199 156 107 84 22 21
SrfI GCCC'GGGC
Cuts at: 0 344 1392
Size: 344 1048
StuI AGG'CCT
Cuts at: 0 773 1392
Size: 773 619
StyI C'CwwG_G
Cuts at: 0 260 269 1392
Size: 260 9 1123
Fragments arranged by size:
1123 260 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 675 801 983 1046 1067 1088 1154 1196
Size: 675 126 182 63 21 21 66 42
Cuts at: 1196 1392
Size: 196
Fragments arranged by size:
675 196 182 126 66 63 42 21
21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 139 171 650 682 1392
Size: 139 32 479 32 710
Fragments arranged by size:
710 479 139 32 32
XmaI C'CCGG_G
Cuts at: 0 19 342 822 1021 1128 1212 1234 1392
Size: 19 323 480 199 107 84 22 158
Fragments arranged by size:
480 323 199 158 107 84 22 19
Enzymes that do cut:
AlfI AloI AlwNI ApaI ApaLI AvaI BanI BanII
BbvCI BcgI BglI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BstAPI Bsu36I EaeI EagI EarI EciI Eco57MI
EcoO109I FalI HaeII Hin4I MslI MspA1I PasI PflMI
PfoI PpiI PpuMI PspOMI PvuII RsrII SfiI SmaI
SrfI StuI StyI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BciVI BclI BfrBI
BglII BlpI BmtI BplI BpuEI BsaAI BsaBI BsgI
BsmBI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PshAI
PsiI PspXI PsrI PstI PvuI SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SspI SwaI TatI
Tth111I XbaI XcmI XhoI XmnI ZraI