Restriction map for
pFN21AE1516
(Linear) (Six-base) MAPSORT of: KIEE1516.seq Check: 1296 from: 1 to: 4329
KIEE1516.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AatII G_ACGT'C
Cuts at: 0 3680 4329
Size: 3680 649
AccI GT'mk_AC
Cuts at: 0 461 2915 4329
Size: 461 2454 1414
Fragments arranged by size:
2454 1414 461
Acc65I G'GTAC_C
Cuts at: 0 1139 4329
Size: 1139 3190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1481 1709 2029 2247 3980 4329
Size: 364 1117 228 320 218 1733 349
Fragments arranged by size:
1733 1117 364 349 320 228 218
AflIII A'CryG_T
Cuts at: 0 116 707 1601 3842 3926 4329
Size: 116 591 894 2241 84 403
Fragments arranged by size:
2241 894 591 403 116 84
AgeI A'CCGG_T
Cuts at: 0 34 4329
Size: 34 4295
AleI CACnn'nnGTG
Cuts at: 0 3911 4329
Size: 3911 418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1897 3712 3901 4054 4329
Size: 1897 1815 189 153 275
Fragments arranged by size:
1897 1815 275 189 153
AlwNI CAG_nnn'CTG
Cuts at: 0 624 804 4329
Size: 624 180 3525
Fragments arranged by size:
3525 624 180
ApaI G_GGCC'C
Cuts at: 0 585 1813 2151 2772 3512 4329
Size: 585 1228 338 621 740 817
Fragments arranged by size:
1228 817 740 621 585 338
ApaLI G'TGCA_C
Cuts at: 0 751 4329
Size: 751 3578
AvaI C'yCGr_G
Cuts at: 0 196 327 1135 1480 2161 2771 3445 4329
Size: 196 131 808 345 681 610 674 884
Fragments arranged by size:
884 808 681 674 610 345 196 131
BanI G'GyrC_C
Cuts at: 0 331 1139 1308 1927 2351 2374 3441 3913
Size: 331 808 169 619 424 23 1067 472
Cuts at: 3913 4267 4329
Size: 354 62
Fragments arranged by size:
1067 808 619 472 424 354 331 169
62 23
BanII G_rGCy'C
Cuts at: 0 527 585 668 1136 1208 1813 2151 2162
Size: 527 58 83 468 72 605 338 11
Cuts at: 2162 2200 2257 2772 2880 3512 3968 4329
Size: 38 57 515 108 632 456 361
Fragments arranged by size:
632 605 527 515 468 456 361 338
108 83 72 58 57 38 11
BbeI G_GCGC'C
Cuts at: 0 1931 4329
Size: 1931 2398
BbsI GAAGACnn'nnnn_
Cuts at: 0 352 1745 2820 3316 4066 4329
Size: 352 1393 1075 496 750 263
Fragments arranged by size:
1393 1075 750 496 352 263
BbvCI CC'TCA_GC
Cuts at: 0 509 2110 2986 4329
Size: 509 1601 876 1343
Fragments arranged by size:
1601 1343 876 509
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 221 4329
Size: 187 34 4108
Fragments arranged by size:
4108 187 34
BclI T'GATC_A
Cuts at: 0 3419 3644 4329
Size: 3419 225 685
Fragments arranged by size:
3419 685 225
BglI GCCn_nnn'nGGC
Cuts at: 0 2174 3353 4329
Size: 2174 1179 976
Fragments arranged by size:
2174 1179 976
BglII A'GATC_T
Cuts at: 0 1028 2384 4329
Size: 1028 1356 1945
Fragments arranged by size:
1945 1356 1028
BlpI GC'TnA_GC
Cuts at: 0 3727 3967 4329
Size: 3727 240 362
Fragments arranged by size:
3727 362 240
Bme1580I G_kGCm'C
Cuts at: 0 334 585 755 815 1663 1813 2151 2772
Size: 334 251 170 60 848 150 338 621
Cuts at: 2772 3135 3446 3512 3918 4329
Size: 363 311 66 406 411
Fragments arranged by size:
848 621 411 406 363 338 334 311
251 170 150 66 60
BmgBI CAC'GTC
Cuts at: 0 576 1992 4329
Size: 576 1416 2337
Fragments arranged by size:
2337 1416 576
BmrI ACTGGGnnnn_n'
Cuts at: 0 1350 1879 4329
Size: 1350 529 2450
Fragments arranged by size:
2450 1350 529
BmtI G_CTAG'C
Cuts at: 0 4255 4329
Size: 4255 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1269 3237 4329
Size: 1269 1968 1092
Fragments arranged by size:
1968 1269 1092
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1513 3778 3954 4156 4321 4329
Size: 61 1452 2265 176 202 165 8
Fragments arranged by size:
2265 1452 202 176 165 61 8
Bpu10I CC'TnA_GC
Cuts at: 0 286 377 509 2110 2986 4228 4329
Size: 286 91 132 1601 876 1242 101
Fragments arranged by size:
1601 1242 876 286 132 101 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 1165 4329
Size: 1000 165 3164
Fragments arranged by size:
3164 1000 165
BsaI GGTCTCn'nnnn_
Cuts at: 0 2099 3166 3328 4329
Size: 2099 1067 162 1001
Fragments arranged by size:
2099 1067 1001 162
BsaAI yAC'GTr
Cuts at: 0 117 710 1035 3084 3360 3401 3909 3921
Size: 117 593 325 2049 276 41 508 12
Cuts at: 3921 4007 4155 4329
Size: 86 148 174
Fragments arranged by size:
2049 593 508 325 276 174 148 117
86 41 12
BsaBI GATnn'nnATC
Cuts at: 0 1006 3418 4329
Size: 1006 2412 911
Fragments arranged by size:
2412 1006 911
BsaHI Gr'CG_yC
Cuts at: 0 137 1928 3677 4329
Size: 137 1791 1749 652
Fragments arranged by size:
1791 1749 652 137
BsaWI w'CCGG_w
Cuts at: 0 34 4142 4329
Size: 34 4108 187
Fragments arranged by size:
4108 187 34
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 697 727 1123 1153 2224 2254 3151 3181
Size: 697 30 396 30 1071 30 897 30
Cuts at: 3181 3325 3355 3385 3415 4329
Size: 144 30 30 30 914
Fragments arranged by size:
1071 914 897 697 396 144 30 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 526 640 1165 1168 1648 1651 2242
Size: 385 141 114 525 3 480 3 591
Cuts at: 2242 2245 2293 2518 2770 3172 3346 3406 4329
Size: 3 48 225 252 402 174 60 923
Fragments arranged by size:
923 591 525 480 402 385 252 225
174 141 114 60 48 3 3 3
BseYI C'CCAG_C
Cuts at: 0 559 1675 1825 2269 2559 2764 3206 3248
Size: 559 1116 150 444 290 205 442 42
Cuts at: 3248 3794 3798 4329
Size: 546 4 531
Fragments arranged by size:
1116 559 546 531 444 442 290 205
150 42 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 343 418 467 777 1888 1960 2035
Size: 271 72 75 49 310 1111 72 75
Cuts at: 2035 2050 2306 3042 4135 4329
Size: 15 256 736 1093 194
Fragments arranged by size:
1111 1093 736 310 271 256 194 75
75 72 72 49 15
BsiEI CG_ry'CG
Cuts at: 0 1097 1283 1707 2871 2915 3825 3983 4329
Size: 1097 186 424 1164 44 910 158 346
Fragments arranged by size:
1164 1097 910 424 346 186 158 44
BsiHKAI G_wGCw'C
Cuts at: 0 169 518 715 755 2162 4234 4329
Size: 169 349 197 40 1407 2072 95
Fragments arranged by size:
2072 1407 349 197 169 95 40
BsmI GAATG_Cn'
Cuts at: 0 4182 4329
Size: 4182 147
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4297 4329
Size: 4297 32
Bsp1286I G_dGCh'C
Cuts at: 0 169 334 518 527 585 668 715 755
Size: 169 165 184 9 58 83 47 40
Cuts at: 755 815 1136 1208 1663 1813 2151 2162 2200
Size: 60 321 72 455 150 338 11 38
Cuts at: 2200 2257 2772 2880 3135 3446 3512 3918 3968
Size: 57 515 108 255 311 66 406 50
Cuts at: 3968 4234 4329
Size: 266 95
Fragments arranged by size:
515 455 406 338 321 311 266 255
184 169 165 150 108 95 83 72
66 60 58 57 50 47 40 38
11 9
BspEI T'CCGG_A
Cuts at: 0 4142 4329
Size: 4142 187
BspHI T'CATG_A
Cuts at: 0 1973 3680 4329
Size: 1973 1707 649
Fragments arranged by size:
1973 1707 649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 623 2594 3310 3382 3857 4329
Size: 623 1971 716 72 475 472
Fragments arranged by size:
1971 716 623 475 472 72
BsrBI CCG'CTC
Cuts at: 0 3192 4329
Size: 3192 1137
BsrDI GCAATG_nn'
Cuts at: 0 3540 4329
Size: 3540 789
BsrFI r'CCGG_y
Cuts at: 0 34 1282 2866 4067 4120 4329
Size: 34 1248 1584 1201 53 209
Fragments arranged by size:
1584 1248 1201 209 53 34
BsrGI T'GTAC_A
Cuts at: 0 11 405 3764 3839 4329
Size: 11 394 3359 75 490
Fragments arranged by size:
3359 490 394 75 11
BssSI C'ACGA_G
Cuts at: 0 517 526 4329
Size: 517 9 3803
Fragments arranged by size:
3803 517 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 624 750 4329
Size: 624 126 3579
Fragments arranged by size:
3579 624 126
BstEII G'GTnAC_C
Cuts at: 0 1122 2862 4329
Size: 1122 1740 1467
Fragments arranged by size:
1740 1467 1122
BstXI CCAn_nnnn'nTGG
Cuts at: 0 39 1119 3535 3756 4329
Size: 39 1080 2416 221 573
Fragments arranged by size:
2416 1080 573 221 39
BstYI r'GATC_y
Cuts at: 0 1028 2384 3044 3413 4329
Size: 1028 1356 660 369 916
Fragments arranged by size:
1356 1028 916 660 369
BtgI C'CryG_G
Cuts at: 0 239 920 1127 1436 1515 1814 1916 1949
Size: 239 681 207 309 79 299 102 33
Cuts at: 1949 2378 2894 2952 3028 4235 4329
Size: 429 516 58 76 1207 94
Fragments arranged by size:
1207 681 516 429 309 299 239 207
102 94 79 76 58 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1599 4329
Size: 1599 2730
BtsI GCAGTG_nn'
Cuts at: 0 355 1071 1948 2178 3333 3528 4329
Size: 355 716 877 230 1155 195 801
Fragments arranged by size:
1155 877 801 716 355 230 195
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1803 1838 4329
Size: 1803 35 2491
Fragments arranged by size:
2491 1803 35
DraIII CAC_nnn'GTG
Cuts at: 0 3072 3128 3546 4329
Size: 3072 56 418 783
Fragments arranged by size:
3072 783 418 56
DrdI GACnn_nn'nnGTC
Cuts at: 0 1003 2911 4329
Size: 1003 1908 1418
Fragments arranged by size:
1908 1418 1003
EaeI y'GGCC_r
Cuts at: 0 95 242 692 722 1094 1211 1280 1373
Size: 95 147 450 30 372 117 69 93
Cuts at: 1373 1512 1704 1952 2028 2649 2868 2897 3107
Size: 139 192 248 76 621 219 29 210
Cuts at: 3107 3362 3453 3537 3551 3558 4329
Size: 255 91 84 14 7 771
Fragments arranged by size:
771 621 450 372 255 248 219 210
192 147 139 117 95 93 91 84
76 69 30 29 14 7
EagI C'GGCC_G
Cuts at: 0 1094 1280 1704 2868 4329
Size: 1094 186 424 1164 1461
Fragments arranged by size:
1461 1164 1094 424 186
EarI CTCTTCn'nnn_
Cuts at: 0 1238 1457 1543 2005 2266 3679 4329
Size: 1238 219 86 462 261 1413 650
Fragments arranged by size:
1413 1238 650 462 261 219 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 17 1867 3426 4329
Size: 17 1850 1559 903
Fragments arranged by size:
1850 1559 903 17
EcoICRI GAG'CTC
Cuts at: 0 2160 4329
Size: 2160 2169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 364 1481 1513 1709 2029 2247 3778
Size: 61 303 1117 32 196 320 218 1531
Cuts at: 3778 3954 3980 4156 4321 4329
Size: 176 26 176 165 8
Fragments arranged by size:
1531 1117 320 303 218 196 176 176
165 61 32 26 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 492 508 2536 3382 4329
Size: 492 16 2028 846 947
Fragments arranged by size:
2028 947 846 492 16
EcoO109I rG'GnC_Cy
Cuts at: 0 101 582 1809 1810 2147 2190 2316 2448
Size: 101 481 1227 1 337 43 126 132
Cuts at: 2448 2579 2682 2768 3371 3508 3938 4329
Size: 131 103 86 603 137 430 391
Fragments arranged by size:
1227 603 481 430 391 337 137 132
131 126 103 101 86 43 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2629 4329
Size: 2629 1700
FspI TGC'GCA
Cuts at: 0 1063 3325 4186 4329
Size: 1063 2262 861 143
Fragments arranged by size:
2262 1063 861 143
HaeII r_GCGC'y
Cuts at: 0 444 1931 2050 2560 4329
Size: 444 1487 119 510 1769
Fragments arranged by size:
1769 1487 510 444 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 697 729 1342 1374 2239 2271 3303 3325
Size: 697 32 613 32 865 32 1032 22
Cuts at: 3325 3335 3357 3398 3430 4329
Size: 10 22 41 32 899
Fragments arranged by size:
1032 899 865 697 613 41 32 32
32 32 22 22 10
HincII GTy'rAC
Cuts at: 0 849 2916 2943 4329
Size: 849 2067 27 1386
Fragments arranged by size:
2067 1386 849 27
HindIII A'AGCT_T
Cuts at: 0 288 4329
Size: 288 4041
KasI G'GCGC_C
Cuts at: 0 1927 4329
Size: 1927 2402
KpnI G_GTAC'C
Cuts at: 0 1143 4329
Size: 1143 3186
MscI TGG'CCA
Cuts at: 0 244 724 1375 2899 3109 3455 3539 4329
Size: 244 480 651 1524 210 346 84 790
Fragments arranged by size:
1524 790 651 480 346 244 210 84
MslI CAynn'nnrTG
Cuts at: 0 238 574 1117 1915 3427 3533 3911 4329
Size: 238 336 543 798 1512 106 378 418
Fragments arranged by size:
1512 798 543 418 378 336 238 106
MspA1I CmG'CkG
Cuts at: 0 255 1294 1371 1902 2064 2114 2445 2954
Size: 255 1039 77 531 162 50 331 509
Cuts at: 2954 3051 3248 3798 4329
Size: 97 197 550 531
Fragments arranged by size:
1039 550 531 531 509 331 255 197
162 97 77 50
NaeI GCC'GGC
Cuts at: 0 1284 4122 4329
Size: 1284 2838 207
Fragments arranged by size:
2838 1284 207
NarI GG'CG_CC
Cuts at: 0 1928 4329
Size: 1928 2401
NcoI C'CATG_G
Cuts at: 0 239 1127 2378 2894 3028 4235 4329
Size: 239 888 1251 516 134 1207 94
Fragments arranged by size:
1251 1207 888 516 239 134 94
NgoMIV G'CCGG_C
Cuts at: 0 1282 4120 4329
Size: 1282 2838 209
Fragments arranged by size:
2838 1282 209
NheI G'CTAG_C
Cuts at: 0 4251 4329
Size: 4251 78
NotI GC'GGCC_GC
Cuts at: 0 1094 4329
Size: 1094 3235
NspI r_CATG'y
Cuts at: 0 1062 1605 2121 2599 3298 3846 3882 4329
Size: 1062 543 516 478 699 548 36 447
Fragments arranged by size:
1062 699 548 543 516 478 447 36
PciI A'CATG_T
Cuts at: 0 1601 3842 4329
Size: 1601 2241 487
Fragments arranged by size:
2241 1601 487
PflMI CCAn_nnn'nTGG
Cuts at: 0 804 2043 3072 4329
Size: 804 1239 1029 1257
Fragments arranged by size:
1257 1239 1029 804
PfoI T'CCnGG_A
Cuts at: 0 4286 4329
Size: 4286 43
PmlI CAC'GTG
Cuts at: 0 710 3084 3401 3909 3921 4007 4155 4329
Size: 710 2374 317 508 12 86 148 174
Fragments arranged by size:
2374 710 508 317 174 148 86 12
PpuMI rG'GwC_Cy
Cuts at: 0 101 2316 2448 2682 3371 3938 4329
Size: 101 2215 132 234 689 567 391
Fragments arranged by size:
2215 689 567 391 234 132 101
PspOMI G'GGCC_C
Cuts at: 0 581 1809 2147 2768 3508 4329
Size: 581 1228 338 621 740 821
Fragments arranged by size:
1228 821 740 621 581 338
PspXI vC'TCGA_Gb
Cuts at: 0 196 4329
Size: 196 4133
PstI C_TGCA'G
Cuts at: 0 620 1202 1547 1760 3284 4088 4329
Size: 620 582 345 213 1524 804 241
Fragments arranged by size:
1524 804 620 582 345 241 213
PvuII CAG'CTG
Cuts at: 0 255 1294 1371 1902 3248 3798 4329
Size: 255 1039 77 531 1346 550 531
Fragments arranged by size:
1346 1039 550 531 531 255 77
RsrII CG'GwC_CG
Cuts at: 0 4308 4329
Size: 4308 21
SacI G_AGCT'C
Cuts at: 0 2162 4329
Size: 2162 2167
SacII CC_GC'GG
Cuts at: 0 2955 4329
Size: 2955 1374
SalI G'TCGA_C
Cuts at: 0 2914 4329
Size: 2914 1415
SanDI GG'GwC_CC
Cuts at: 0 2316 4329
Size: 2316 2013
SapI GCTCTTCn'nnn_
Cuts at: 0 1238 1543 3679 4329
Size: 1238 305 2136 650
Fragments arranged by size:
2136 1238 650 305
SexAI A'CCwGG_T
Cuts at: 0 86 3542 4329
Size: 86 3456 787
Fragments arranged by size:
3456 787 86
SfcI C'TryA_G
Cuts at: 0 616 1198 1543 1756 3280 4084 4329
Size: 616 582 345 213 1524 804 245
Fragments arranged by size:
1524 804 616 582 345 245 213
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2174 4329
Size: 2174 2155
SfoI GGC'GCC
Cuts at: 0 1929 4329
Size: 1929 2400
SmaI CCC'GGG
Cuts at: 0 329 1137 2773 4329
Size: 329 808 1636 1556
Fragments arranged by size:
1636 1556 808 329
SmlI C'TyrA_G
Cuts at: 0 196 1015 1180 4329
Size: 196 819 165 3149
Fragments arranged by size:
3149 819 196 165
SphI G_CATG'C
Cuts at: 0 1062 3882 4329
Size: 1062 2820 447
Fragments arranged by size:
2820 1062 447
StuI AGG'CCT
Cuts at: 0 2626 3217 4329
Size: 2626 591 1112
Fragments arranged by size:
2626 1112 591
StyI C'CwwG_G
Cuts at: 0 239 1127 1649 2078 2378 2894 3028 3860
Size: 239 888 522 429 300 516 134 832
Cuts at: 3860 4235 4329
Size: 375 94
Fragments arranged by size:
888 832 522 516 429 375 300 239
134 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 349 520 811 955 2131 3901 3969 4329
Size: 349 171 291 144 1176 1770 68 360
Fragments arranged by size:
1770 1176 360 349 291 171 144 68
TatI w'GTAC_w
Cuts at: 0 11 53 405 1184 2963 3764 3839 4329
Size: 11 42 352 779 1779 801 75 490
Fragments arranged by size:
1779 801 779 490 352 75 42 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1859 1891 2059 2091 3385 3417 4329
Size: 1859 32 168 32 1294 32 912
Fragments arranged by size:
1859 1294 912 168 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 351 3315 3339 4312 4329
Size: 351 2964 24 973 17
Fragments arranged by size:
2964 973 351 24 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 309 679 828 1434 4329
Size: 252 57 370 149 606 2895
Fragments arranged by size:
2895 606 370 252 149 57
XhoI C'TCGA_G
Cuts at: 0 196 4329
Size: 196 4133
XmaI C'CCGG_G
Cuts at: 0 327 1135 2771 4329
Size: 327 808 1636 1558
Fragments arranged by size:
1636 1558 808 327
XmnI GAAnn'nnTTC
Cuts at: 0 1466 2428 3475 3568 3928 4329
Size: 1466 962 1047 93 360 401
Fragments arranged by size:
1466 1047 962 401 360 93
ZraI GAC'GTC
Cuts at: 0 3678 4329
Size: 3678 651
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NspI
PciI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI
PvuII RsrII SacI SacII SalI SanDI SapI SexAI
SfcI SfiI SfoI SmaI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AhdI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BsiWI
BssHII BstBI BstZ17I Bsu36I ClaI DraI EcoRI EcoRV
FseI FspAI HpaI MfeI MluI MmeI NdeI NruI
NsiI PacI PasI PmeI PpiI PshAI PsiI PsrI
PvuI SbfI ScaI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI XbaI