Restriction map for
pFN21AE1512
(Linear) (Six-base) MAPSORT of: KIEE1512.seq Check: 3565 from: 1 to: 1170
KIEE1512.orf
With 182 enzymes: SgfI *
April 24, 2012 14:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 1170
Size: 322 848
AflIII A'CryG_T
Cuts at: 0 832 890 1170
Size: 832 58 280
Fragments arranged by size:
832 280 58
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 748 1170
Size: 748 422
ApoI r'AATT_y
Cuts at: 0 3 271 373 565 595 1170
Size: 3 268 102 192 30 575
Fragments arranged by size:
575 268 192 102 30 3
BclI T'GATC_A
Cuts at: 0 177 1170
Size: 177 993
BfrBI ATG'CAT
Cuts at: 0 292 1170
Size: 292 878
BglII A'GATC_T
Cuts at: 0 717 1170
Size: 717 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 691 975 1170
Size: 401 290 284 195
Fragments arranged by size:
401 290 284 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 1170
Size: 868 302
BsaI GGTCTCn'nnnn_
Cuts at: 0 970 1170
Size: 970 200
BsaBI GATnn'nnATC
Cuts at: 0 1161 1170
Size: 1161 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 948 978 1170
Size: 948 30 192
Fragments arranged by size:
948 192 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1027 1170
Size: 1027 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 1170
Size: 131 1039
BsrDI GCAATG_nn'
Cuts at: 0 513 1170
Size: 513 657
BsrGI T'GTAC_A
Cuts at: 0 627 678 1170
Size: 627 51 492
Fragments arranged by size:
627 492 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 693 909 1170
Size: 693 216 261
Fragments arranged by size:
693 261 216
BstYI r'GATC_y
Cuts at: 0 717 1170
Size: 717 453
BtgI C'CryG_G
Cuts at: 0 902 960 1170
Size: 902 58 210
Fragments arranged by size:
902 210 58
BtsI GCAGTG_nn'
Cuts at: 0 143 559 782 1170
Size: 143 416 223 388
Fragments arranged by size:
416 388 223 143
ClaI AT'CG_AT
Cuts at: 0 752 1170
Size: 752 418
DraI TTT'AAA
Cuts at: 0 570 1170
Size: 570 600
DraIII CAC_nnn'GTG
Cuts at: 0 13 1170
Size: 13 1157
EaeI y'GGCC_r
Cuts at: 0 601 1170
Size: 601 569
EarI CTCTTCn'nnn_
Cuts at: 0 325 767 863 1076 1150 1170
Size: 325 442 96 213 74 20
Fragments arranged by size:
442 325 213 96 74 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 401 691 975 1170
Size: 322 79 290 284 195
Fragments arranged by size:
322 290 284 195 79
EcoNI CCTnn'n_nnAGG
Cuts at: 0 988 1170
Size: 988 182
EcoO109I rG'GnC_Cy
Cuts at: 0 690 1170
Size: 690 480
EcoRI G'AATT_C
Cuts at: 0 3 271 1170
Size: 3 268 899
Fragments arranged by size:
899 268 3
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 948 980 1170
Size: 948 32 190
Fragments arranged by size:
948 190 32
HindIII A'AGCT_T
Cuts at: 0 259 766 1170
Size: 259 507 404
Fragments arranged by size:
507 404 259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 1170
Size: 72 1098
MscI TGG'CCA
Cuts at: 0 603 1170
Size: 603 567
MslI CAynn'nnrTG
Cuts at: 0 730 907 1170
Size: 730 177 263
Fragments arranged by size:
730 263 177
MspA1I CmG'CkG
Cuts at: 0 1033 1042 1170
Size: 1033 9 128
Fragments arranged by size:
1033 128 9
NcoI C'CATG_G
Cuts at: 0 902 1170
Size: 902 268
NdeI CA'TA_TG
Cuts at: 0 294 1170
Size: 294 876
NsiI A_TGCA'T
Cuts at: 0 294 1170
Size: 294 876
NspI r_CATG'y
Cuts at: 0 836 1170
Size: 836 334
PciI A'CATG_T
Cuts at: 0 832 1170
Size: 832 338
PfoI T'CCnGG_A
Cuts at: 0 692 1170
Size: 692 478
PpuMI rG'GwC_Cy
Cuts at: 0 690 1170
Size: 690 480
PshAI GACnn'nnGTC
Cuts at: 0 835 1170
Size: 835 335
PstI C_TGCA'G
Cuts at: 0 764 1032 1170
Size: 764 268 138
Fragments arranged by size:
764 268 138
PvuII CAG'CTG
Cuts at: 0 1033 1170
Size: 1033 137
SapI GCTCTTCn'nnn_
Cuts at: 0 325 1170
Size: 325 845
SfcI C'TryA_G
Cuts at: 0 760 1028 1043 1170
Size: 760 268 15 127
Fragments arranged by size:
760 268 127 15
SmlI C'TyrA_G
Cuts at: 0 883 1170
Size: 883 287
SspI AAT'ATT
Cuts at: 0 355 1170
Size: 355 815
StuI AGG'CCT
Cuts at: 0 994 1170
Size: 994 176
StyI C'CwwG_G
Cuts at: 0 686 902 1170
Size: 686 216 268
Fragments arranged by size:
686 268 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 438 1170
Size: 438 732
TatI w'GTAC_w
Cuts at: 0 627 678 1170
Size: 627 51 492
Fragments arranged by size:
627 492 51
XmnI GAAnn'nnTTC
Cuts at: 0 919 1170
Size: 919 251
Enzymes that do cut:
AcuI AflIII AlfI ApoI BclI BfrBI BglII BpmI
BpuEI BsaI BsaBI BsaXI BseRI BsgI BsrDI BsrGI
BstXI BstYI BtgI BtsI ClaI DraI DraIII EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I HindIII MmeI
MscI MslI MspA1I NcoI NdeI NsiI NspI PciI
PfoI PpuMI PshAI PstI PvuII SapI SfcI SmlI
SspI StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BsaAI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI CspCI DrdI EagI EciI EcoICRI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI PacI PasI PflMI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI