Restriction map for
pFN21AE1511
(Linear) (Six-base) MAPSORT of: KIEE1511.seq Check: 1369 from: 1 to: 966
KIEE1511.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 623 966
Size: 466 157 343
Fragments arranged by size:
466 343 157
AlwNI CAG_nnn'CTG
Cuts at: 0 730 966
Size: 730 236
AvaI C'yCGr_G
Cuts at: 0 920 966
Size: 920 46
BanII G_rGCy'C
Cuts at: 0 39 966
Size: 39 927
BbsI GAAGACnn'nnnn_
Cuts at: 0 856 966
Size: 856 110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 723 757 966
Size: 723 34 209
Fragments arranged by size:
723 209 34
BmgBI CAC'GTC
Cuts at: 0 543 966
Size: 543 423
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 787 966
Size: 787 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 555 966
Size: 130 425 411
Fragments arranged by size:
425 411 130
BsaI GGTCTCn'nnnn_
Cuts at: 0 290 775 966
Size: 290 485 191
Fragments arranged by size:
485 290 191
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 77 966
Size: 77 889
BseYI C'CCAG_C
Cuts at: 0 288 966
Size: 288 678
BsiHKAI G_wGCw'C
Cuts at: 0 39 966
Size: 39 927
BsmBI CGTCTCn'nnnn_
Cuts at: 0 533 966
Size: 533 433
Bsp1286I G_dGCh'C
Cuts at: 0 39 966
Size: 39 927
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 966
Size: 164 802
BssSI C'ACGA_G
Cuts at: 0 59 966
Size: 59 907
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 197 232 966
Size: 197 35 734
Fragments arranged by size:
734 197 35
EaeI y'GGCC_r
Cuts at: 0 327 966
Size: 327 639
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 308 966
Size: 308 658
EcoICRI GAG'CTC
Cuts at: 0 37 966
Size: 37 929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 623 966
Size: 466 157 343
Fragments arranged by size:
466 343 157
EcoNI CCTnn'n_nnAGG
Cuts at: 0 445 966
Size: 445 521
EcoO109I rG'GnC_Cy
Cuts at: 0 79 153 966
Size: 79 74 813
Fragments arranged by size:
813 79 74
EcoRV GAT'ATC
Cuts at: 0 384 966
Size: 384 582
HaeII r_GCGC'y
Cuts at: 0 8 966
Size: 8 958
HincII GTy'rAC
Cuts at: 0 699 966
Size: 699 267
MfeI C'AATT_G
Cuts at: 0 311 966
Size: 311 655
MscI TGG'CCA
Cuts at: 0 329 966
Size: 329 637
MslI CAynn'nnrTG
Cuts at: 0 364 966
Size: 364 602
MspA1I CmG'CkG
Cuts at: 0 237 966
Size: 237 729
PasI CC'CwG_GG
Cuts at: 0 550 843 966
Size: 550 293 123
Fragments arranged by size:
550 293 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 306 730 966
Size: 306 424 236
Fragments arranged by size:
424 306 236
PpuMI rG'GwC_Cy
Cuts at: 0 153 966
Size: 153 813
PvuII CAG'CTG
Cuts at: 0 237 966
Size: 237 729
SacI G_AGCT'C
Cuts at: 0 39 966
Size: 39 927
SfcI C'TryA_G
Cuts at: 0 83 966
Size: 83 883
SmlI C'TyrA_G
Cuts at: 0 109 534 966
Size: 109 425 432
Fragments arranged by size:
432 425 109
StyI C'CwwG_G
Cuts at: 0 208 512 899 966
Size: 208 304 387 67
Fragments arranged by size:
387 304 208 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 354 480 966
Size: 354 126 486
Fragments arranged by size:
486 354 126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 349 485 966
Size: 349 136 481
Fragments arranged by size:
481 349 136
Enzymes that do cut:
AcuI AlwNI AvaI BanII BbsI BcgI BmgBI BplI
BpuEI BsaI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI
BssSI CspCI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV HaeII HincII MfeI MscI MslI MspA1I PasI
PflMI PpuMI PvuII SacI SfcI SmlI StyI TaqII
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BmtI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI
BsaXI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EagI EarI EcoRI FalI FseI FspI
FspAI Hin4I HindIII HpaI KasI KpnI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI