(Linear) (Six-base) MAPSORT of: KIEE1507.seq  Check: 3918  from: 1  to: 885



KIEE1507.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:40  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577     664     885 
   Size:        577      87     221

  Fragments arranged by size: 

                 577     221      87
 
AleI CACnn'nnGTG

Cuts at:      0     645     885 
   Size:        645     240
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     585     885 
   Size:        585     300
 
AlwNI CAG_nnn'CTG

Cuts at:      0      36     885 
   Size:         36     849
 
ApoI r'AATT_y

Cuts at:      0     231     885 
   Size:        231     654
 
AseI AT'TA_AT

Cuts at:      0     530     885 
   Size:        530     355
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     670     885 
   Size:        670     215
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     744     885 
   Size:        744     141
 
BglII A'GATC_T

Cuts at:      0     171     708     885 
   Size:        171     537     177

  Fragments arranged by size: 

                 537     177     171
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     418     448     885 
   Size:        418      30     437

  Fragments arranged by size: 

                 437     418      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     803     885 
   Size:        101     702      82

  Fragments arranged by size: 

                 702     101      82
 
BsmI GAATG_Cn'

Cuts at:      0      57     885 
   Size:         57     828
 
BsrDI GCAATG_nn'

Cuts at:      0     567     620     885 
   Size:        567      53     265

  Fragments arranged by size: 

                 567     265      53
 
BstYI r'GATC_y

Cuts at:      0     171     632     708     885 
   Size:        171     461      76     177

  Fragments arranged by size: 

                 461     177     171      76
 
EarI CTCTTCn'nnn_

Cuts at:      0     323     885 
   Size:        323     562
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577     664     885 
   Size:        577      87     221

  Fragments arranged by size: 

                 577     221      87
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     193     885 
   Size:        193     692
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     204     236     718     750     885 
   Size:        204      32     482      32     135

  Fragments arranged by size: 

                 482     204     135      32      32
 
HincII GTy'rAC

Cuts at:      0     659     885 
   Size:        659     226
 
HpaI GTT'AAC

Cuts at:      0     659     885 
   Size:        659     226
 
MfeI C'AATT_G

Cuts at:      0     826     885 
   Size:        826      59
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     270     418     885 
   Size:         41     229     148     467

  Fragments arranged by size: 

                 467     229     148      41
 
MslI CAynn'nnrTG

Cuts at:      0     125     645     885 
   Size:        125     520     240

  Fragments arranged by size: 

                 520     240     125
 
MspA1I CmG'CkG

Cuts at:      0      48     885 
   Size:         48     837
 
NspI r_CATG'y

Cuts at:      0     566     885 
   Size:        566     319
 
PvuII CAG'CTG

Cuts at:      0      48     885 
   Size:         48     837
 
SfcI C'TryA_G

Cuts at:      0     843     885 
   Size:        843      42
 
SspI AAT'ATT

Cuts at:      0     754     885 
   Size:        754     131
 
StyI C'CwwG_G

Cuts at:      0     279     885 
   Size:        279     606
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     272     885 
   Size:        272     613
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     418     450     885 
   Size:        418      32     435

  Fragments arranged by size: 

                 435     418      32
 
XbaI T'CTAG_A

Cuts at:      0      73     885 
   Size:         73     812
 
XmnI GAAnn'nnTTC

Cuts at:      0     677     885 
   Size:        677     208
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI     ApoI     AseI     BbsI    BciVI
    BglII    BsaXI     BsgI     BsmI    BsrDI    BstYI     EarI  Eco57MI
     FalI    Hin4I   HincII     HpaI     MfeI     MmeI     MslI   MspA1I
     NspI    PvuII     SfcI     SspI     StyI    TaqII     TstI     XbaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII  HindIII     KasI     KpnI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TatI  Tth111I     XcmI
     XhoI     XmaI     ZraI