Restriction map for
pFN21AE1506
(Linear) (Six-base) MAPSORT of: KIEE1506.seq Check: 8820 from: 1 to: 1083
KIEE1506.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 267 1083
Size: 267 816
AccI GT'mk_AC
Cuts at: 0 1010 1083
Size: 1010 73
Acc65I G'GTAC_C
Cuts at: 0 964 1083
Size: 964 119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 999 1078 1083
Size: 999 79 5
Fragments arranged by size:
999 79 5
AflII C'TTAA_G
Cuts at: 0 216 1083
Size: 216 867
AleI CACnn'nnGTG
Cuts at: 0 241 981 1083
Size: 241 740 102
Fragments arranged by size:
740 241 102
ApaI G_GGCC'C
Cuts at: 0 107 1083
Size: 107 976
ApaLI G'TGCA_C
Cuts at: 0 64 1083
Size: 64 1019
ApoI r'AATT_y
Cuts at: 0 169 514 563 1083
Size: 169 345 49 520
Fragments arranged by size:
520 345 169 49
AvaI C'yCGr_G
Cuts at: 0 76 1039 1083
Size: 76 963 44
Fragments arranged by size:
963 76 44
BanI G'GyrC_C
Cuts at: 0 964 1083
Size: 964 119
BanII G_rGCy'C
Cuts at: 0 107 369 1083
Size: 107 262 714
Fragments arranged by size:
714 262 107
Bme1580I G_kGCm'C
Cuts at: 0 68 107 1083
Size: 68 39 976
Fragments arranged by size:
976 68 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 1083
Size: 836 247
Bpu10I CC'TnA_GC
Cuts at: 0 554 749 1083
Size: 554 195 334
Fragments arranged by size:
554 334 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1083
Size: 889 194
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 1083
Size: 79 1004
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 650 1022 1083
Size: 472 178 372 61
Fragments arranged by size:
472 372 178 61
BseYI C'CCAG_C
Cuts at: 0 572 1083
Size: 572 511
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 1083
Size: 959 124
BsiHKAI G_wGCw'C
Cuts at: 0 68 1083
Size: 68 1015
BsmI GAATG_Cn'
Cuts at: 0 640 1083
Size: 640 443
Bsp1286I G_dGCh'C
Cuts at: 0 68 107 369 1083
Size: 68 39 262 714
Fragments arranged by size:
714 262 68 39
BspHI T'CATG_A
Cuts at: 0 956 1083
Size: 956 127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 267 793 1083
Size: 267 526 290
Fragments arranged by size:
526 290 267
BsrDI GCAATG_nn'
Cuts at: 0 527 1083
Size: 527 556
BsrGI T'GTAC_A
Cuts at: 0 731 1083
Size: 731 352
BstAPI GCAn_nnn'nTGC
Cuts at: 0 63 1083
Size: 63 1020
BstBI TT'CG_AA
Cuts at: 0 561 1083
Size: 561 522
BtgI C'CryG_G
Cuts at: 0 11 107 149 1083
Size: 11 96 42 934
Fragments arranged by size:
934 96 42 11
BtsI GCAGTG_nn'
Cuts at: 0 236 765 894 1083
Size: 236 529 129 189
Fragments arranged by size:
529 236 189 129
DraI TTT'AAA
Cuts at: 0 429 921 1083
Size: 429 492 162
Fragments arranged by size:
492 429 162
DraIII CAC_nnn'GTG
Cuts at: 0 199 1083
Size: 199 884
EarI CTCTTCn'nnn_
Cuts at: 0 149 1083
Size: 149 934
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 999 1078 1083
Size: 836 163 79 5
Fragments arranged by size:
836 163 79 5
EcoO109I rG'GnC_Cy
Cuts at: 0 81 103 1083
Size: 81 22 980
Fragments arranged by size:
980 81 22
EcoRI G'AATT_C
Cuts at: 0 514 563 1083
Size: 514 49 520
Fragments arranged by size:
520 514 49
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 429 1029 1083
Size: 429 600 54
Fragments arranged by size:
600 429 54
HincII GTy'rAC
Cuts at: 0 1076 1083
Size: 1076 7
HindIII A'AGCT_T
Cuts at: 0 1066 1083
Size: 1066 17
HpaI GTT'AAC
Cuts at: 0 1076 1083
Size: 1076 7
KpnI G_GTAC'C
Cuts at: 0 968 1083
Size: 968 115
MfeI C'AATT_G
Cuts at: 0 830 1083
Size: 830 253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1083
Size: 394 689
MslI CAynn'nnrTG
Cuts at: 0 241 691 981 1083
Size: 241 450 290 102
Fragments arranged by size:
450 290 241 102
MspA1I CmG'CkG
Cuts at: 0 60 783 1083
Size: 60 723 300
Fragments arranged by size:
723 300 60
PasI CC'CwG_GG
Cuts at: 0 100 1083
Size: 100 983
PpuMI rG'GwC_Cy
Cuts at: 0 81 1083
Size: 81 1002
PspOMI G'GGCC_C
Cuts at: 0 103 1083
Size: 103 980
PstI C_TGCA'G
Cuts at: 0 59 1083
Size: 59 1024
PvuII CAG'CTG
Cuts at: 0 60 783 1083
Size: 60 723 300
Fragments arranged by size:
723 300 60
SfcI C'TryA_G
Cuts at: 0 55 979 1083
Size: 55 924 104
Fragments arranged by size:
924 104 55
SmlI C'TyrA_G
Cuts at: 0 216 904 1083
Size: 216 688 179
Fragments arranged by size:
688 216 179
StuI AGG'CCT
Cuts at: 0 632 1083
Size: 632 451
StyI C'CwwG_G
Cuts at: 0 378 1083
Size: 378 705
TatI w'GTAC_w
Cuts at: 0 158 293 731 1083
Size: 158 135 438 352
Fragments arranged by size:
438 352 158 135
XmnI GAAnn'nnTTC
Cuts at: 0 37 1083
Size: 37 1046
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AleI ApaI ApaLI
ApoI AvaI BanI BanII Bme1580I BpmI Bpu10I BpuEI
BsaI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrGI BstAPI BstBI BtgI BtsI DraI
DraIII EarI Eco57MI EcoO109I EcoRI FalI HincII HindIII
HpaI KpnI MfeI MmeI MslI MspA1I PasI PpuMI
PspOMI PstI PvuII SfcI SmlI StuI StyI TatI
XmnI
Enzymes that do not cut:
AatII AclI AfeI AflIII AgeI AhdI AlfI AloI
AlwNI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI
BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI
BssHII BssSI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRV FseI FspI FspAI HaeII Hin4I KasI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI ZraI