Restriction map for
pFN21AE1505
(Linear) (Six-base) MAPSORT of: KIEE1505.seq Check: 6503 from: 1 to: 1488
KIEE1505.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 597 732 1488
Size: 597 135 756
Fragments arranged by size:
756 597 135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 1042 1488
Size: 523 519 446
Fragments arranged by size:
523 519 446
AflIII A'CryG_T
Cuts at: 0 833 1488
Size: 833 655
AleI CACnn'nnGTG
Cuts at: 0 541 1488
Size: 541 947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 559 932 1237 1488
Size: 559 373 305 251
Fragments arranged by size:
559 373 305 251
AlwNI CAG_nnn'CTG
Cuts at: 0 153 1020 1068 1290 1488
Size: 153 867 48 222 198
Fragments arranged by size:
867 222 198 153 48
ApaLI G'TGCA_C
Cuts at: 0 668 1488
Size: 668 820
AvaI C'yCGr_G
Cuts at: 0 6 1477 1488
Size: 6 1471 11
Fragments arranged by size:
1471 11 6
BanI G'GyrC_C
Cuts at: 0 1053 1228 1353 1384 1488
Size: 1053 175 125 31 104
Fragments arranged by size:
1053 175 125 104 31
BbeI G_GCGC'C
Cuts at: 0 1232 1488
Size: 1232 256
BbsI GAAGACnn'nnnn_
Cuts at: 0 453 1179 1488
Size: 453 726 309
Fragments arranged by size:
726 453 309
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 616 650 1488
Size: 616 34 838
Fragments arranged by size:
838 616 34
BglI GCCn_nnn'nGGC
Cuts at: 0 586 1488
Size: 586 902
BlpI GC'TnA_GC
Cuts at: 0 1450 1488
Size: 1450 38
Bme1580I G_kGCm'C
Cuts at: 0 672 1048 1488
Size: 672 376 440
Fragments arranged by size:
672 440 376
BmgBI CAC'GTC
Cuts at: 0 405 651 1488
Size: 405 246 837
Fragments arranged by size:
837 405 246
BmrI ACTGGGnnnn_n'
Cuts at: 0 555 682 1213 1488
Size: 555 127 531 275
Fragments arranged by size:
555 531 275 127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 936 1488
Size: 936 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1351 1462 1488
Size: 163 1188 111 26
Fragments arranged by size:
1188 163 111 26
Bpu10I CC'TnA_GC
Cuts at: 0 1283 1488
Size: 1283 205
BsaI GGTCTCn'nnnn_
Cuts at: 0 1330 1488
Size: 1330 158
BsaHI Gr'CG_yC
Cuts at: 0 1229 1488
Size: 1229 259
BsaWI w'CCGG_w
Cuts at: 0 20 1397 1488
Size: 20 1377 91
Fragments arranged by size:
1377 91 20
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 919 949 1488
Size: 919 30 539
Fragments arranged by size:
919 539 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 643 1348 1488
Size: 643 705 140
Fragments arranged by size:
705 643 140
BseYI C'CCAG_C
Cuts at: 0 594 1290 1488
Size: 594 696 198
Fragments arranged by size:
696 594 198
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 625 766 1027 1243 1273 1488
Size: 145 480 141 261 216 30 215
Fragments arranged by size:
480 261 216 215 145 141 30
BsiHKAI G_wGCw'C
Cuts at: 0 13 548 672 878 1488
Size: 13 535 124 206 610
Fragments arranged by size:
610 535 206 124 13
Bsp1286I G_dGCh'C
Cuts at: 0 13 548 672 878 1048 1488
Size: 13 535 124 206 170 440
Fragments arranged by size:
535 440 206 170 124 13
BspEI T'CCGG_A
Cuts at: 0 1397 1488
Size: 1397 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 597 732 1173 1445 1488
Size: 597 135 441 272 43
Fragments arranged by size:
597 441 272 135 43
BsrBI CCG'CTC
Cuts at: 0 795 1364 1488
Size: 795 569 124
Fragments arranged by size:
795 569 124
BsrFI r'CCGG_y
Cuts at: 0 517 1050 1339 1488
Size: 517 533 289 149
Fragments arranged by size:
533 517 289 149
BssHII G'CGCG_C
Cuts at: 0 1278 1488
Size: 1278 210
BssSI C'ACGA_G
Cuts at: 0 701 1488
Size: 701 787
BstAPI GCAn_nnn'nTGC
Cuts at: 0 153 1017 1143 1211 1488
Size: 153 864 126 68 277
Fragments arranged by size:
864 277 153 126 68
BstBI TT'CG_AA
Cuts at: 0 788 1488
Size: 788 700
BstEII G'GTnAC_C
Cuts at: 0 16 1488
Size: 16 1472
BtgI C'CryG_G
Cuts at: 0 842 966 1264 1488
Size: 842 124 298 224
Fragments arranged by size:
842 298 224 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 497 1488
Size: 497 991
BtsI GCAGTG_nn'
Cuts at: 0 113 608 690 1010 1148 1488
Size: 113 495 82 320 138 340
Fragments arranged by size:
495 340 320 138 113 82
DrdI GACnn_nn'nnGTC
Cuts at: 0 1081 1488
Size: 1081 407
EaeI y'GGCC_r
Cuts at: 0 515 578 653 1160 1488
Size: 515 63 75 507 328
Fragments arranged by size:
515 507 328 75 63
EarI CTCTTCn'nnn_
Cuts at: 0 785 1488
Size: 785 703
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 337 826 1488
Size: 337 489 662
Fragments arranged by size:
662 489 337
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 523 1042 1351 1462 1488
Size: 163 360 519 309 111 26
Fragments arranged by size:
519 360 309 163 111 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 247 625 1330 1488
Size: 247 378 705 158
Fragments arranged by size:
705 378 247 158
EcoO109I rG'GnC_Cy
Cuts at: 0 362 1065 1488
Size: 362 703 423
Fragments arranged by size:
703 423 362
FspI TGC'GCA
Cuts at: 0 1118 1488
Size: 1118 370
HaeII r_GCGC'y
Cuts at: 0 646 1232 1488
Size: 646 586 256
Fragments arranged by size:
646 586 256
HincII GTy'rAC
Cuts at: 0 270 1488
Size: 270 1218
HpaI GTT'AAC
Cuts at: 0 270 1488
Size: 270 1218
KasI G'GCGC_C
Cuts at: 0 1228 1488
Size: 1228 260
MslI CAynn'nnrTG
Cuts at: 0 541 883 1348 1488
Size: 541 342 465 140
Fragments arranged by size:
541 465 342 140
MspA1I CmG'CkG
Cuts at: 0 390 844 968 1211 1257 1266 1377 1488
Size: 390 454 124 243 46 9 111 111
Fragments arranged by size:
454 390 243 124 111 111 46 9
NaeI GCC'GGC
Cuts at: 0 519 1488
Size: 519 969
NarI GG'CG_CC
Cuts at: 0 1229 1488
Size: 1229 259
NgoMIV G'CCGG_C
Cuts at: 0 517 1488
Size: 517 971
PflMI CCAn_nnn'nTGG
Cuts at: 0 255 357 1488
Size: 255 102 1131
Fragments arranged by size:
1131 255 102
PfoI T'CCnGG_A
Cuts at: 0 395 1328 1488
Size: 395 933 160
Fragments arranged by size:
933 395 160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 946 978 1488
Size: 946 32 510
Fragments arranged by size:
946 510 32
PspXI vC'TCGA_Gb
Cuts at: 0 6 1477 1488
Size: 6 1471 11
Fragments arranged by size:
1471 11 6
PstI C_TGCA'G
Cuts at: 0 1005 1488
Size: 1005 483
PvuII CAG'CTG
Cuts at: 0 390 1211 1257 1488
Size: 390 821 46 231
Fragments arranged by size:
821 390 231 46
SacII CC_GC'GG
Cuts at: 0 845 969 1267 1488
Size: 845 124 298 221
Fragments arranged by size:
845 298 221 124
SapI GCTCTTCn'nnn_
Cuts at: 0 785 1488
Size: 785 703
ScaI AGT'ACT
Cuts at: 0 1483 1488
Size: 1483 5
SexAI A'CCwGG_T
Cuts at: 0 1076 1488
Size: 1076 412
SfcI C'TryA_G
Cuts at: 0 366 1001 1488
Size: 366 635 487
Fragments arranged by size:
635 487 366
SfoI GGC'GCC
Cuts at: 0 1230 1488
Size: 1230 258
SgrAI Cr'CCGG_yG
Cuts at: 0 1050 1488
Size: 1050 438
SmlI C'TyrA_G
Cuts at: 0 6 1477 1488
Size: 6 1471 11
Fragments arranged by size:
1471 11 6
StyI C'CwwG_G
Cuts at: 0 134 938 1163 1418 1488
Size: 134 804 225 255 70
Fragments arranged by size:
804 255 225 134 70
TatI w'GTAC_w
Cuts at: 0 1235 1481 1488
Size: 1235 246 7
Fragments arranged by size:
1235 246 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 919 951 1488
Size: 919 32 537
Fragments arranged by size:
919 537 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 945 1488
Size: 945 543
XhoI C'TCGA_G
Cuts at: 0 6 1477 1488
Size: 6 1471 11
Fragments arranged by size:
1471 11 6
XmnI GAAnn'nnTTC
Cuts at: 0 175 1488
Size: 175 1313
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaLI AvaI
BanI BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BtgI BtgZI
BtsI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FspI HaeII HincII HpaI KasI MslI MspA1I NaeI
NarI NgoMIV PflMI PfoI PpiI PspXI PstI PvuII
SacII SapI ScaI SexAI SfcI SfoI SgrAI SmlI
StyI TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbvCI BciVI BclI BfrBI BglII BmtI
BpuEI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspHI
BsrDI BsrGI BstXI BstYI BstZ17I Bsu36I ClaI CspCI
DraI DraIII EagI EcoICRI EcoRI EcoRV FalI FseI
FspAI Hin4I HindIII KpnI MfeI MluI MmeI MscI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpuMI PshAI PsiI PspOMI
PsrI PvuI RsrII SacI SalI SanDI SbfI SfiI
SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XmaI ZraI