Restriction map for
pFN21AE1502
(Linear) (Six-base) MAPSORT of: KIEE1502.seq Check: 5745 from: 1 to: 1095
KIEE1502.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 712 1095
Size: 712 383
AfeI AGC'GCT
Cuts at: 0 757 1095
Size: 757 338
AflIII A'CryG_T
Cuts at: 0 774 1095
Size: 774 321
AhdI GACnn_n'nnGTC
Cuts at: 0 543 1095
Size: 543 552
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 820 1095
Size: 820 275
AlwNI CAG_nnn'CTG
Cuts at: 0 119 717 956 1088 1095
Size: 119 598 239 132 7
Fragments arranged by size:
598 239 132 119 7
ApaI G_GGCC'C
Cuts at: 0 423 1095
Size: 423 672
AvaI C'yCGr_G
Cuts at: 0 25 197 211 1095
Size: 25 172 14 884
Fragments arranged by size:
884 172 25 14
BanI G'GyrC_C
Cuts at: 0 202 343 405 448 766 784 983 1095
Size: 202 141 62 43 318 18 199 112
Fragments arranged by size:
318 202 199 141 112 62 43 18
BanII G_rGCy'C
Cuts at: 0 423 478 1082 1095
Size: 423 55 604 13
Fragments arranged by size:
604 423 55 13
BbeI G_GCGC'C
Cuts at: 0 206 347 409 770 1095
Size: 206 141 62 361 325
Fragments arranged by size:
361 325 206 141 62
BbvCI CC'TCA_GC
Cuts at: 0 949 1095
Size: 949 146
BglI GCCn_nnn'nGGC
Cuts at: 0 414 446 473 1095
Size: 414 32 27 622
Fragments arranged by size:
622 414 32 27
BlpI GC'TnA_GC
Cuts at: 0 170 1081 1095
Size: 170 911 14
Fragments arranged by size:
911 170 14
Bme1580I G_kGCm'C
Cuts at: 0 55 423 787 986 1095
Size: 55 368 364 199 109
Fragments arranged by size:
368 364 199 109 55
BmgBI CAC'GTC
Cuts at: 0 63 525 1095
Size: 63 462 570
Fragments arranged by size:
570 462 63
BmtI G_CTAG'C
Cuts at: 0 196 1095
Size: 196 899
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 865 1095
Size: 865 230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 772 1095
Size: 246 526 323
Fragments arranged by size:
526 323 246
Bpu10I CC'TnA_GC
Cuts at: 0 949 1095
Size: 949 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 1095
Size: 811 284
BsaHI Gr'CG_yC
Cuts at: 0 203 344 406 767 1095
Size: 203 141 62 361 328
Fragments arranged by size:
361 328 203 141 62
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 841 1095
Size: 841 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1021 1095
Size: 595 426 74
Fragments arranged by size:
595 426 74
BsiEI CG_ry'CG
Cuts at: 0 341 921 978 1095
Size: 341 580 57 117
Fragments arranged by size:
580 341 117 57
BsiHKAI G_wGCw'C
Cuts at: 0 1082 1095
Size: 1082 13
BsmBI CGTCTCn'nnnn_
Cuts at: 0 304 1095
Size: 304 791
Bsp1286I G_dGCh'C
Cuts at: 0 55 423 478 787 986 1082 1095
Size: 55 368 55 309 199 96 13
Fragments arranged by size:
368 309 199 96 55 55 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 712 971 1095
Size: 712 259 124
Fragments arranged by size:
712 259 124
BsrBI CCG'CTC
Cuts at: 0 844 1095
Size: 844 251
BsrFI r'CCGG_y
Cuts at: 0 340 898 1095
Size: 340 558 197
Fragments arranged by size:
558 340 197
BssHII G'CGCG_C
Cuts at: 0 692 740 1095
Size: 692 48 355
Fragments arranged by size:
692 355 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 662 717 1095
Size: 662 55 378
Fragments arranged by size:
662 378 55
BtgI C'CryG_G
Cuts at: 0 411 470 672 762 978 1095
Size: 411 59 202 90 216 117
Fragments arranged by size:
411 216 202 117 90 59
BtsI GCAGTG_nn'
Cuts at: 0 332 397 1095
Size: 332 65 698
Fragments arranged by size:
698 332 65
DraIII CAC_nnn'GTG
Cuts at: 0 662 1095
Size: 662 433
EaeI y'GGCC_r
Cuts at: 0 140 338 635 975 1095
Size: 140 198 297 340 120
Fragments arranged by size:
340 297 198 140 120
EagI C'GGCC_G
Cuts at: 0 338 975 1095
Size: 338 637 120
Fragments arranged by size:
637 338 120
EarI CTCTTCn'nnn_
Cuts at: 0 1004 1095
Size: 1004 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 83 235 1095
Size: 83 152 860
Fragments arranged by size:
860 152 83
EcoICRI GAG'CTC
Cuts at: 0 1080 1095
Size: 1080 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 772 1095
Size: 246 526 323
Fragments arranged by size:
526 323 246
EcoNI CCTnn'n_nnAGG
Cuts at: 0 295 1095
Size: 295 800
EcoO109I rG'GnC_Cy
Cuts at: 0 420 801 1095
Size: 420 381 294
Fragments arranged by size:
420 381 294
FspI TGC'GCA
Cuts at: 0 700 1072 1095
Size: 700 372 23
Fragments arranged by size:
700 372 23
HaeII r_GCGC'y
Cuts at: 0 206 347 409 494 759 770 941 1095
Size: 206 141 62 85 265 11 171 154
Fragments arranged by size:
265 206 171 154 141 85 62 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 1095
Size: 529 32 534
Fragments arranged by size:
534 529 32
KasI G'GCGC_C
Cuts at: 0 202 343 405 766 1095
Size: 202 141 62 361 329
Fragments arranged by size:
361 329 202 141 62
MluI A'CGCG_T
Cuts at: 0 774 1095
Size: 774 321
MspA1I CmG'CkG
Cuts at: 0 251 413 472 953 980 1061 1095
Size: 251 162 59 481 27 81 34
Fragments arranged by size:
481 251 162 81 59 34 27
NaeI GCC'GGC
Cuts at: 0 342 1095
Size: 342 753
NarI GG'CG_CC
Cuts at: 0 203 344 406 767 1095
Size: 203 141 62 361 328
Fragments arranged by size:
361 328 203 141 62
NgoMIV G'CCGG_C
Cuts at: 0 340 1095
Size: 340 755
NheI G'CTAG_C
Cuts at: 0 192 1095
Size: 192 903
NotI GC'GGCC_GC
Cuts at: 0 975 1095
Size: 975 120
NruI TCG'CGA
Cuts at: 0 731 1095
Size: 731 364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 1095
Size: 214 32 849
Fragments arranged by size:
849 214 32
PspOMI G'GGCC_C
Cuts at: 0 419 1095
Size: 419 676
PstI C_TGCA'G
Cuts at: 0 124 392 1095
Size: 124 268 703
Fragments arranged by size:
703 268 124
SacI G_AGCT'C
Cuts at: 0 1082 1095
Size: 1082 13
SacII CC_GC'GG
Cuts at: 0 414 473 981 1095
Size: 414 59 508 114
Fragments arranged by size:
508 414 114 59
SfcI C'TryA_G
Cuts at: 0 120 388 1095
Size: 120 268 707
Fragments arranged by size:
707 268 120
SfoI GGC'GCC
Cuts at: 0 204 345 407 768 1095
Size: 204 141 62 361 327
Fragments arranged by size:
361 327 204 141 62
SmlI C'TyrA_G
Cuts at: 0 826 1095
Size: 826 269
StuI AGG'CCT
Cuts at: 0 277 367 1095
Size: 277 90 728
Fragments arranged by size:
728 277 90
StyI C'CwwG_G
Cuts at: 0 95 1095
Size: 95 1000
TatI w'GTAC_w
Cuts at: 0 1047 1095
Size: 1047 48
Enzymes that do cut:
AarI AfeI AflIII AhdI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmgBI
BmtI BplI BpmI Bpu10I BpuEI BsaHI BseRI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BstAPI BtgI BtsI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI
MluI MspA1I NaeI NarI NgoMIV NheI NotI NruI
PpiI PspOMI PstI SacI SacII SfcI SfoI SmlI
StuI StyI TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BcgI BciVI BclI BfrBI BglII BmrI
BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KpnI MfeI MmeI MscI MslI NcoI NdeI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI