(Linear) (Six-base) MAPSORT of: KIEE1496.seq  Check: 5113  from: 1  to: 324



KIEE1496.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:40  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     324 
   Size:        176     148
 
AfeI AGC'GCT

Cuts at:      0      61     324 
   Size:         61     263
 
ApoI r'AATT_y

Cuts at:      0     304     324 
   Size:        304      20
 
AvaI C'yCGr_G

Cuts at:      0      27     324 
   Size:         27     297
 
BanI G'GyrC_C

Cuts at:      0      34     324 
   Size:         34     290
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      15     324 
   Size:         15     309
 
BglI GCCn_nnn'nGGC

Cuts at:      0      97     324 
   Size:         97     227
 
Bme1580I G_kGCm'C

Cuts at:      0     323     324 
   Size:        323       1
 
BsaHI Gr'CG_yC

Cuts at:      0     181     324 
   Size:        181     143
 
BsmI GAATG_Cn'

Cuts at:      0      87     324 
   Size:         87     237
 
Bsp1286I G_dGCh'C

Cuts at:      0     323     324 
   Size:        323       1
 
BsrBI CCG'CTC

Cuts at:      0      32     324 
   Size:         32     292
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     316     324 
   Size:        316       8
 
BstYI r'GATC_y

Cuts at:      0     150     324 
   Size:        150     174
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      25     324 
   Size:         25     299
 
BtsI GCAGTG_nn'

Cuts at:      0     243     324 
   Size:        243      81
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     278     324 
   Size:        278      46
 
EarI CTCTTCn'nnn_

Cuts at:      0     191     324 
   Size:        191     133
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     324 
   Size:        176     148
 
EcoRI G'AATT_C

Cuts at:      0     304     324 
   Size:        304      20
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      78     324 
   Size:         78     246
 
HaeII r_GCGC'y

Cuts at:      0      63     324 
   Size:         63     261
 
MspA1I CmG'CkG

Cuts at:      0      44     324 
   Size:         44     280
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     147     324 
   Size:        147     177
 
PstI C_TGCA'G

Cuts at:      0     136     324 
   Size:        136     188
 
SfcI C'TryA_G

Cuts at:      0     132     324 
   Size:        132     192
 
TatI w'GTAC_w

Cuts at:      0     202     324 
   Size:        202     122
 

 Enzymes that do cut:

     AcuI     AfeI     ApoI     AvaI     BanI    BciVI     BglI Bme1580I
    BsaHI     BsmI Bsp1286I    BsrBI    BstXI    BstYI    BtgZI     BtsI
     DrdI     EarI  Eco57MI    EcoRI     FalI    HaeII   MspA1I    PflMI
     PstI     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI     DraI   DraIII     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI