Restriction map for
pFN21AE1494
(Linear) (Six-base) MAPSORT of: KIEE1494.seq Check: 4054 from: 1 to: 741
KIEE1494.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 741
Size: 157 584
ApaI G_GGCC'C
Cuts at: 0 228 741
Size: 228 513
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 531 564 741
Size: 531 33 177
Fragments arranged by size:
531 177 33
BanII G_rGCy'C
Cuts at: 0 228 741
Size: 228 513
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 490 524 741
Size: 490 34 217
Fragments arranged by size:
490 217 34
BglI GCCn_nnn'nGGC
Cuts at: 0 381 741
Size: 381 360
Bme1580I G_kGCm'C
Cuts at: 0 228 741
Size: 228 513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 629 741
Size: 361 268 112
Fragments arranged by size:
361 268 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 684 741
Size: 684 57
BseYI C'CCAG_C
Cuts at: 0 254 437 741
Size: 254 183 304
Fragments arranged by size:
304 254 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 520 672 741
Size: 391 129 152 69
Fragments arranged by size:
391 152 129 69
Bsp1286I G_dGCh'C
Cuts at: 0 228 741
Size: 228 513
BsrFI r'CCGG_y
Cuts at: 0 433 741
Size: 433 308
BsrGI T'GTAC_A
Cuts at: 0 708 741
Size: 708 33
BssSI C'ACGA_G
Cuts at: 0 680 741
Size: 680 61
Bsu36I CC'TnA_GG
Cuts at: 0 379 741
Size: 379 362
BtsI GCAGTG_nn'
Cuts at: 0 432 741
Size: 432 309
EaeI y'GGCC_r
Cuts at: 0 431 741
Size: 431 310
EarI CTCTTCn'nnn_
Cuts at: 0 133 741
Size: 133 608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 361 629 741
Size: 157 204 268 112
Fragments arranged by size:
268 204 157 112
EcoO109I rG'GnC_Cy
Cuts at: 0 224 570 613 741
Size: 224 346 43 128
Fragments arranged by size:
346 224 128 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 609 741
Size: 609 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 108 115 140 147 171 203 741
Size: 108 7 25 7 24 32 538
Fragments arranged by size:
538 108 32 25 24 7 7
HindIII A'AGCT_T
Cuts at: 0 702 741
Size: 702 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 741
Size: 341 400
MspA1I CmG'CkG
Cuts at: 0 184 339 741
Size: 184 155 402
Fragments arranged by size:
402 184 155
NaeI GCC'GGC
Cuts at: 0 435 741
Size: 435 306
NgoMIV G'CCGG_C
Cuts at: 0 433 741
Size: 433 308
NspI r_CATG'y
Cuts at: 0 425 675 741
Size: 425 250 66
Fragments arranged by size:
425 250 66
PasI CC'CwG_GG
Cuts at: 0 41 456 639 741
Size: 41 415 183 102
Fragments arranged by size:
415 183 102 41
PpuMI rG'GwC_Cy
Cuts at: 0 570 741
Size: 570 171
PspOMI G'GGCC_C
Cuts at: 0 224 741
Size: 224 517
PstI C_TGCA'G
Cuts at: 0 338 741
Size: 338 403
PvuII CAG'CTG
Cuts at: 0 184 339 741
Size: 184 155 402
Fragments arranged by size:
402 184 155
SanDI GG'GwC_CC
Cuts at: 0 570 741
Size: 570 171
SfcI C'TryA_G
Cuts at: 0 334 741
Size: 334 407
SmlI C'TyrA_G
Cuts at: 0 699 741
Size: 699 42
SphI G_CATG'C
Cuts at: 0 675 741
Size: 675 66
StyI C'CwwG_G
Cuts at: 0 218 741
Size: 218 523
TatI w'GTAC_w
Cuts at: 0 708 741
Size: 708 33
Enzymes that do cut:
AcuI ApaI BaeI BanII BcgI BglI Bme1580I BpmI
BpuEI BseYI BsgI Bsp1286I BsrFI BsrGI BssSI Bsu36I
BtsI EaeI EarI Eco57MI EcoO109I FalI Hin4I HindIII
MmeI MspA1I NaeI NgoMIV NspI PasI PpuMI PspOMI
PstI PvuII SanDI SfcI SmlI SphI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BamHI BanI BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BssHII BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MscI MslI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI