Restriction map for
pFN21AE1491
(Linear) (Six-base) MAPSORT of: KIEE1491.seq Check: 6592 from: 1 to: 1221
KIEE1491.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AatII G_ACGT'C
Cuts at: 0 196 1221
Size: 196 1025
AccI GT'mk_AC
Cuts at: 0 278 1221
Size: 278 943
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 307 1221
Size: 118 189 914
Fragments arranged by size:
914 189 118
AhdI GACnn_n'nnGTC
Cuts at: 0 192 1221
Size: 192 1029
AlwNI CAG_nnn'CTG
Cuts at: 0 474 706 1017 1093 1221
Size: 474 232 311 76 128
Fragments arranged by size:
474 311 232 128 76
ApaI G_GGCC'C
Cuts at: 0 555 1060 1221
Size: 555 505 161
Fragments arranged by size:
555 505 161
AvaI C'yCGr_G
Cuts at: 0 44 723 1044 1159 1221
Size: 44 679 321 115 62
Fragments arranged by size:
679 321 115 62 44
BanI G'GyrC_C
Cuts at: 0 33 49 252 1221
Size: 33 16 203 969
Fragments arranged by size:
969 203 33 16
BanII G_rGCy'C
Cuts at: 0 555 730 1060 1221
Size: 555 175 330 161
Fragments arranged by size:
555 330 175 161
BbeI G_GCGC'C
Cuts at: 0 53 1221
Size: 53 1168
BbsI GAAGACnn'nnnn_
Cuts at: 0 215 1221
Size: 215 1006
BbvCI CC'TCA_GC
Cuts at: 0 711 1221
Size: 711 510
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 424 458 1221
Size: 424 34 763
Fragments arranged by size:
763 424 34
BclI T'GATC_A
Cuts at: 0 218 1221
Size: 218 1003
BlpI GC'TnA_GC
Cuts at: 0 790 1221
Size: 790 431
Bme1580I G_kGCm'C
Cuts at: 0 265 408 555 1060 1221
Size: 265 143 147 505 161
Fragments arranged by size:
505 265 161 147 143
BmrI ACTGGGnnnn_n'
Cuts at: 0 418 547 592 731 996 1221
Size: 418 129 45 139 265 225
Fragments arranged by size:
418 265 225 139 129 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 603 683 784 1221
Size: 584 19 80 101 437
Fragments arranged by size:
584 437 101 80 19
Bpu10I CC'TnA_GC
Cuts at: 0 711 1221
Size: 711 510
BsaAI yAC'GTr
Cuts at: 0 282 1221
Size: 282 939
BsaHI Gr'CG_yC
Cuts at: 0 50 193 877 1221
Size: 50 143 684 344
Fragments arranged by size:
684 344 143 50
BsaWI w'CCGG_w
Cuts at: 0 117 1221
Size: 117 1104
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 99 129 1221
Size: 99 30 1092
Fragments arranged by size:
1092 99 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 367 603 606 1216 1221
Size: 367 236 3 610 5
Fragments arranged by size:
610 367 236 5 3
BseYI C'CCAG_C
Cuts at: 0 265 477 546 684 776 1053 1221
Size: 265 212 69 138 92 277 168
Fragments arranged by size:
277 265 212 168 138 92 69
Bsp1286I G_dGCh'C
Cuts at: 0 265 408 555 730 1060 1221
Size: 265 143 147 175 330 161
Fragments arranged by size:
330 265 175 161 147 143
BspEI T'CCGG_A
Cuts at: 0 117 1221
Size: 117 1104
BsrDI GCAATG_nn'
Cuts at: 0 357 1221
Size: 357 864
BsrFI r'CCGG_y
Cuts at: 0 523 1221
Size: 523 698
BsrGI T'GTAC_A
Cuts at: 0 140 1221
Size: 140 1081
BssHII G'CGCG_C
Cuts at: 0 14 1221
Size: 14 1207
BstAPI GCAn_nnn'nTGC
Cuts at: 0 474 1221
Size: 474 747
BstEII G'GTnAC_C
Cuts at: 0 645 1221
Size: 645 576
BstXI CCAn_nnnn'nTGG
Cuts at: 0 582 1221
Size: 582 639
BstYI r'GATC_y
Cuts at: 0 977 1221
Size: 977 244
Bsu36I CC'TnA_GG
Cuts at: 0 9 1221
Size: 9 1212
BtgI C'CryG_G
Cuts at: 0 968 1221
Size: 968 253
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1130 1221
Size: 1130 91
BtsI GCAGTG_nn'
Cuts at: 0 265 597 1221
Size: 265 332 624
Fragments arranged by size:
624 332 265
EaeI y'GGCC_r
Cuts at: 0 429 572 812 846 863 1221
Size: 429 143 240 34 17 358
Fragments arranged by size:
429 358 240 143 34 17
EarI CTCTTCn'nnn_
Cuts at: 0 672 1193 1221
Size: 672 521 28
Fragments arranged by size:
672 521 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 307 584 603 683 784 1221
Size: 118 189 277 19 80 101 437
Fragments arranged by size:
437 277 189 118 101 80 19
EcoO109I rG'GnC_Cy
Cuts at: 0 551 552 1014 1057 1221
Size: 551 1 462 43 164
Fragments arranged by size:
551 462 164 43 1
HaeII r_GCGC'y
Cuts at: 0 53 927 1221
Size: 53 874 294
Fragments arranged by size:
874 294 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 876 908 1221
Size: 178 32 666 32 313
Fragments arranged by size:
666 313 178 32 32
HincII GTy'rAC
Cuts at: 0 372 1221
Size: 372 849
KasI G'GCGC_C
Cuts at: 0 49 1221
Size: 49 1172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 1221
Size: 80 1141
MscI TGG'CCA
Cuts at: 0 574 814 865 1221
Size: 574 240 51 356
Fragments arranged by size:
574 356 240 51
MspA1I CmG'CkG
Cuts at: 0 269 419 428 643 706 789 970 1053
Size: 269 150 9 215 63 83 181 83
Cuts at: 1053 1221
Size: 168
Fragments arranged by size:
269 215 181 168 150 83 83 63
9
NaeI GCC'GGC
Cuts at: 0 525 1221
Size: 525 696
NarI GG'CG_CC
Cuts at: 0 50 1221
Size: 50 1171
NgoMIV G'CCGG_C
Cuts at: 0 523 1221
Size: 523 698
NspI r_CATG'y
Cuts at: 0 1026 1221
Size: 1026 195
PasI CC'CwG_GG
Cuts at: 0 1017 1221
Size: 1017 204
PflMI CCAn_nnn'nTGG
Cuts at: 0 855 1221
Size: 855 366
PfoI T'CCnGG_A
Cuts at: 0 196 625 742 917 1221
Size: 196 429 117 175 304
Fragments arranged by size:
429 304 196 175 117
PspOMI G'GGCC_C
Cuts at: 0 551 1056 1221
Size: 551 505 165
Fragments arranged by size:
551 505 165
PstI C_TGCA'G
Cuts at: 0 470 1089 1221
Size: 470 619 132
Fragments arranged by size:
619 470 132
PvuII CAG'CTG
Cuts at: 0 706 789 1053 1221
Size: 706 83 264 168
Fragments arranged by size:
706 264 168 83
SacII CC_GC'GG
Cuts at: 0 971 1221
Size: 971 250
ScaI AGT'ACT
Cuts at: 0 64 1221
Size: 64 1157
SfcI C'TryA_G
Cuts at: 0 466 1085 1221
Size: 466 619 136
Fragments arranged by size:
619 466 136
SfoI GGC'GCC
Cuts at: 0 51 1221
Size: 51 1170
StyI C'CwwG_G
Cuts at: 0 1037 1221
Size: 1037 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 529 1066 1221
Size: 529 537 155
Fragments arranged by size:
537 529 155
TatI w'GTAC_w
Cuts at: 0 62 140 1221
Size: 62 78 1081
Fragments arranged by size:
1081 78 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 99 131 1221
Size: 99 32 1090
Fragments arranged by size:
1090 99 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 63 1221
Size: 63 1158
ZraI GAC'GTC
Cuts at: 0 194 1221
Size: 194 1027
Enzymes that do cut:
AatII AccI AcuI AhdI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BclI BlpI Bme1580I
BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI
BseYI Bsp1286I BspEI BsrDI BsrFI BsrGI BssHII BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI
EarI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI
MscI MspA1I NaeI NarI NgoMIV NspI PasI PflMI
PfoI PspOMI PstI PvuII SacII ScaI SfcI SfoI
StyI TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BfrBI BglI BglII BmgBI BmtI
BplI BpuEI BsaI BsaBI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspHI BspMI BsrBI BssSI BstBI BstZ17I
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MslI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI XmnI