Restriction map for
pFN21AE1489
(Linear) (Six-base) MAPSORT of: KIEE1489.seq Check: 7090 from: 1 to: 1365
KIEE1489.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 508 1365
Size: 508 857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 801 837 997 1365
Size: 257 544 36 160 368
Fragments arranged by size:
544 368 257 160 36
AflIII A'CryG_T
Cuts at: 0 1266 1365
Size: 1266 99
AleI CACnn'nnGTG
Cuts at: 0 631 1365
Size: 631 734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 649 1365
Size: 649 716
AlwNI CAG_nnn'CTG
Cuts at: 0 293 443 1365
Size: 293 150 922
Fragments arranged by size:
922 293 150
ApaLI G'TGCA_C
Cuts at: 0 460 495 570 1365
Size: 460 35 75 795
Fragments arranged by size:
795 460 75 35
ApoI r'AATT_y
Cuts at: 0 1117 1365
Size: 1117 248
AvaI C'yCGr_G
Cuts at: 0 222 1365
Size: 222 1143
AvrII C'CTAG_G
Cuts at: 0 99 1131 1365
Size: 99 1032 234
Fragments arranged by size:
1032 234 99
BanI G'GyrC_C
Cuts at: 0 625 942 1127 1365
Size: 625 317 185 238
Fragments arranged by size:
625 317 238 185
BbeI G_GCGC'C
Cuts at: 0 1131 1365
Size: 1131 234
BbsI GAAGACnn'nnnn_
Cuts at: 0 1348 1365
Size: 1348 17
BbvCI CC'TCA_GC
Cuts at: 0 298 1365
Size: 298 1067
BciVI GTATCCnnnnn_n'
Cuts at: 0 225 1365
Size: 225 1140
BglII A'GATC_T
Cuts at: 0 338 1365
Size: 338 1027
BlpI GC'TnA_GC
Cuts at: 0 1138 1365
Size: 1138 227
Bme1580I G_kGCm'C
Cuts at: 0 464 499 574 613 866 1100 1365
Size: 464 35 75 39 253 234 265
Fragments arranged by size:
464 265 253 234 75 39 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 860 1346 1365
Size: 860 486 19
Fragments arranged by size:
860 486 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 276 555 1365
Size: 276 279 810
Fragments arranged by size:
810 279 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 586 812 867 1183 1239 1273 1321
Size: 67 519 226 55 316 56 34 48
Cuts at: 1321 1365
Size: 44
Fragments arranged by size:
519 316 226 67 56 55 48 44
34
Bpu10I CC'TnA_GC
Cuts at: 0 298 1365
Size: 298 1067
BsaHI Gr'CG_yC
Cuts at: 0 1128 1365
Size: 1128 237
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 26 56 1256 1286 1365
Size: 26 30 1200 30 79
Fragments arranged by size:
1200 79 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 670 1327 1365
Size: 670 657 38
Fragments arranged by size:
670 657 38
BseYI C'CCAG_C
Cuts at: 0 1260 1290 1365
Size: 1260 30 75
Fragments arranged by size:
1260 75 30
BsiEI CG_ry'CG
Cuts at: 0 1158 1365
Size: 1158 207
BsiHKAI G_wGCw'C
Cuts at: 0 44 464 499 574 1274 1365
Size: 44 420 35 75 700 91
Fragments arranged by size:
700 420 91 75 44 35
Bsp1286I G_dGCh'C
Cuts at: 0 44 464 499 574 613 866 1100 1274
Size: 44 420 35 75 39 253 234 174
Cuts at: 1274 1365
Size: 91
Fragments arranged by size:
420 253 234 174 91 75 44 39
35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 499 508 1295 1365
Size: 32 467 9 787 70
Fragments arranged by size:
787 467 70 32 9
BsrBI CCG'CTC
Cuts at: 0 257 1365
Size: 257 1108
BsrFI r'CCGG_y
Cuts at: 0 395 1365
Size: 395 970
BsrGI T'GTAC_A
Cuts at: 0 203 1365
Size: 203 1162
BssSI C'ACGA_G
Cuts at: 0 1147 1365
Size: 1147 218
BstAPI GCAn_nnn'nTGC
Cuts at: 0 443 1365
Size: 443 922
BstYI r'GATC_y
Cuts at: 0 338 1365
Size: 338 1027
Bsu36I CC'TnA_GG
Cuts at: 0 72 1365
Size: 72 1293
BtgI C'CryG_G
Cuts at: 0 1082 1234 1365
Size: 1082 152 131
Fragments arranged by size:
1082 152 131
BtsI GCAGTG_nn'
Cuts at: 0 291 1365
Size: 291 1074
ClaI AT'CG_AT
Cuts at: 0 1154 1365
Size: 1154 211
DraIII CAC_nnn'GTG
Cuts at: 0 371 494 1365
Size: 371 123 871
Fragments arranged by size:
871 371 123
EarI CTCTTCn'nnn_
Cuts at: 0 109 348 688 1365
Size: 109 239 340 677
Fragments arranged by size:
677 340 239 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 257 586 801 812 837 867 997
Size: 67 190 329 215 11 25 30 130
Cuts at: 997 1183 1239 1273 1321 1365
Size: 186 56 34 48 44
Fragments arranged by size:
329 215 190 186 130 67 56 48
44 34 30 25 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 98 1189 1365
Size: 98 1091 176
Fragments arranged by size:
1091 176 98
EcoRI G'AATT_C
Cuts at: 0 1117 1365
Size: 1117 248
HaeII r_GCGC'y
Cuts at: 0 1034 1131 1182 1321 1365
Size: 1034 97 51 139 44
Fragments arranged by size:
1034 139 97 51 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 281 313 1256 1288 1365
Size: 281 32 943 32 77
Fragments arranged by size:
943 281 77 32 32
HindIII A'AGCT_T
Cuts at: 0 813 1365
Size: 813 552
KasI G'GCGC_C
Cuts at: 0 1127 1365
Size: 1127 238
MfeI C'AATT_G
Cuts at: 0 432 1365
Size: 432 933
MluI A'CGCG_T
Cuts at: 0 1266 1365
Size: 1266 99
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 1365
Size: 1016 349
MslI CAynn'nnrTG
Cuts at: 0 631 1365
Size: 631 734
MspA1I CmG'CkG
Cuts at: 0 302 1236 1365
Size: 302 934 129
Fragments arranged by size:
934 302 129
NarI GG'CG_CC
Cuts at: 0 1128 1365
Size: 1128 237
NspI r_CATG'y
Cuts at: 0 1209 1365
Size: 1209 156
PasI CC'CwG_GG
Cuts at: 0 846 1365
Size: 846 519
PfoI T'CCnGG_A
Cuts at: 0 44 1365
Size: 44 1321
PstI C_TGCA'G
Cuts at: 0 244 382 1172 1365
Size: 244 138 790 193
Fragments arranged by size:
790 244 193 138
PvuI CG_AT'CG
Cuts at: 0 1158 1365
Size: 1158 207
PvuII CAG'CTG
Cuts at: 0 302 1365
Size: 302 1063
SacII CC_GC'GG
Cuts at: 0 1237 1365
Size: 1237 128
SfcI C'TryA_G
Cuts at: 0 240 378 543 1168 1365
Size: 240 138 165 625 197
Fragments arranged by size:
625 240 197 165 138
SfoI GGC'GCC
Cuts at: 0 1129 1365
Size: 1129 236
SmaI CCC'GGG
Cuts at: 0 224 1365
Size: 224 1141
SphI G_CATG'C
Cuts at: 0 1209 1365
Size: 1209 156
StyI C'CwwG_G
Cuts at: 0 99 135 1131 1365
Size: 99 36 996 234
Fragments arranged by size:
996 234 99 36
TatI w'GTAC_w
Cuts at: 0 203 1365
Size: 203 1162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 26 58 355 387 1365
Size: 26 32 297 32 978
Fragments arranged by size:
978 297 32 32 26
XmaI C'CCGG_G
Cuts at: 0 222 1365
Size: 222 1143
XmnI GAAnn'nnTTC
Cuts at: 0 1117 1365
Size: 1117 248
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaLI ApoI
AvaI AvrII BanI BbeI BbsI BbvCI BciVI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaHI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtsI ClaI
DraIII EarI Eco57MI EcoNI EcoRI HaeII Hin4I HindIII
KasI MfeI MluI MmeI MslI MspA1I NarI NspI
PasI PfoI PstI PvuI PvuII SacII SfcI SfoI
SmaI SphI StyI TatI TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI AscI AseI AsiSI BaeI BamHI BanII
BcgI BclI BfrBI BglI BmgBI BmtI BpuEI BsaI
BsaAI BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BssHII BstBI BstEII BstXI BstZ17I BtgZI
CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoO109I
EcoRV FalI FseI FspI FspAI HincII HpaI KpnI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI ZraI