Restriction map for
pFN21AE1488
(Linear) (Six-base) MAPSORT of: KIEE1488.seq Check: 3110 from: 1 to: 855
KIEE1488.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 855
Size: 91 764
AflII C'TTAA_G
Cuts at: 0 46 855
Size: 46 809
AleI CACnn'nnGTG
Cuts at: 0 187 855
Size: 187 668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 464 855
Size: 464 391
BanII G_rGCy'C
Cuts at: 0 291 855
Size: 291 564
BglI GCCn_nnn'nGGC
Cuts at: 0 295 855
Size: 295 560
Bme1580I G_kGCm'C
Cuts at: 0 531 855
Size: 531 324
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 790 855
Size: 790 65
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 325 341 357 855
Size: 299 26 16 16 498
Fragments arranged by size:
498 299 26 16 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 855
Size: 413 442
Bsp1286I G_dGCh'C
Cuts at: 0 291 531 855
Size: 291 240 324
Fragments arranged by size:
324 291 240
BsrDI GCAATG_nn'
Cuts at: 0 746 855
Size: 746 109
BsrFI r'CCGG_y
Cuts at: 0 298 855
Size: 298 557
BstXI CCAn_nnnn'nTGG
Cuts at: 0 658 855
Size: 658 197
BstYI r'GATC_y
Cuts at: 0 481 855
Size: 481 374
BtgI C'CryG_G
Cuts at: 0 94 326 499 620 855
Size: 94 232 173 121 235
Fragments arranged by size:
235 232 173 121 94
BtsI GCAGTG_nn'
Cuts at: 0 163 855
Size: 163 692
DraI TTT'AAA
Cuts at: 0 516 855
Size: 516 339
EaeI y'GGCC_r
Cuts at: 0 206 227 855
Size: 206 21 628
Fragments arranged by size:
628 206 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 299 325 341 357 855
Size: 91 208 26 16 16 498
Fragments arranged by size:
498 208 91 26 16 16
EcoO109I rG'GnC_Cy
Cuts at: 0 222 855
Size: 222 633
FseI GG_CCGG'CC
Cuts at: 0 302 855
Size: 302 553
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 323 355 855
Size: 323 32 500
Fragments arranged by size:
500 323 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 801 855
Size: 801 54
MscI TGG'CCA
Cuts at: 0 229 855
Size: 229 626
MslI CAynn'nnrTG
Cuts at: 0 187 855
Size: 187 668
MspA1I CmG'CkG
Cuts at: 0 319 328 742 855
Size: 319 9 414 113
Fragments arranged by size:
414 319 113 9
NaeI GCC'GGC
Cuts at: 0 300 855
Size: 300 555
NcoI C'CATG_G
Cuts at: 0 499 620 855
Size: 499 121 235
Fragments arranged by size:
499 235 121
NgoMIV G'CCGG_C
Cuts at: 0 298 855
Size: 298 557
PfoI T'CCnGG_A
Cuts at: 0 357 855
Size: 357 498
PpuMI rG'GwC_Cy
Cuts at: 0 222 855
Size: 222 633
PstI C_TGCA'G
Cuts at: 0 251 855
Size: 251 604
PvuII CAG'CTG
Cuts at: 0 319 742 855
Size: 319 423 113
Fragments arranged by size:
423 319 113
SacII CC_GC'GG
Cuts at: 0 329 855
Size: 329 526
SexAI A'CCwGG_T
Cuts at: 0 200 855
Size: 200 655
SfcI C'TryA_G
Cuts at: 0 247 855
Size: 247 608
SmlI C'TyrA_G
Cuts at: 0 46 428 855
Size: 46 382 427
Fragments arranged by size:
427 382 46
SspI AAT'ATT
Cuts at: 0 554 855
Size: 554 301
StyI C'CwwG_G
Cuts at: 0 293 499 620 855
Size: 293 206 121 235
Fragments arranged by size:
293 235 206 121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 480 512 855
Size: 480 32 343
Fragments arranged by size:
480 343 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 300 855
Size: 300 555
Enzymes that do cut:
AcuI AflII AleI AlfI BanII BglI Bme1580I BplI
BpmI BpuEI Bsp1286I BsrDI BsrFI BstXI BstYI BtgI
BtsI DraI EaeI Eco57MI EcoO109I FseI Hin4I MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV PfoI PpuMI
PstI PvuII SacII SexAI SfcI SmlI SspI StyI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmrI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI NarI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII TatI Tth111I XbaI XhoI XmaI
XmnI ZraI