Restriction map for
pFN21AE1485
(Linear) (Six-base) MAPSORT of: KIEE1485.seq Check: 1503 from: 1 to: 1269
KIEE1485.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AccI GT'mk_AC
Cuts at: 0 230 1057 1269
Size: 230 827 212
Fragments arranged by size:
827 230 212
AleI CACnn'nnGTG
Cuts at: 0 791 1269
Size: 791 478
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 1269
Size: 295 974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 763 795 1269
Size: 763 32 474
Fragments arranged by size:
763 474 32
ApoI r'AATT_y
Cuts at: 0 263 400 1269
Size: 263 137 869
Fragments arranged by size:
869 263 137
AvaI C'yCGr_G
Cuts at: 0 338 1269
Size: 338 931
BamHI G'GATC_C
Cuts at: 0 448 1269
Size: 448 821
BbsI GAAGACnn'nnnn_
Cuts at: 0 51 1269
Size: 51 1218
BfrBI ATG'CAT
Cuts at: 0 220 927 1269
Size: 220 707 342
Fragments arranged by size:
707 342 220
BglII A'GATC_T
Cuts at: 0 297 1269
Size: 297 972
Bme1580I G_kGCm'C
Cuts at: 0 1171 1269
Size: 1171 98
BmgBI CAC'GTC
Cuts at: 0 291 1269
Size: 291 978
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1118 1269
Size: 1118 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 906 1269
Size: 906 363
BseYI C'CCAG_C
Cuts at: 0 938 1269
Size: 938 331
BsmI GAATG_Cn'
Cuts at: 0 927 1269
Size: 927 342
Bsp1286I G_dGCh'C
Cuts at: 0 1171 1269
Size: 1171 98
BsrBI CCG'CTC
Cuts at: 0 260 1269
Size: 260 1009
BsrDI GCAATG_nn'
Cuts at: 0 744 1269
Size: 744 525
BsrFI r'CCGG_y
Cuts at: 0 165 660 1269
Size: 165 495 609
Fragments arranged by size:
609 495 165
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1024 1269
Size: 1024 245
BstYI r'GATC_y
Cuts at: 0 297 448 554 1115 1269
Size: 297 151 106 561 154
Fragments arranged by size:
561 297 154 151 106
BstZ17I GTA'TAC
Cuts at: 0 1058 1269
Size: 1058 211
Bsu36I CC'TnA_GG
Cuts at: 0 634 1269
Size: 634 635
BtgI C'CryG_G
Cuts at: 0 1068 1269
Size: 1068 201
BtsI GCAGTG_nn'
Cuts at: 0 279 878 1269
Size: 279 599 391
Fragments arranged by size:
599 391 279
DraI TTT'AAA
Cuts at: 0 251 482 1269
Size: 251 231 787
Fragments arranged by size:
787 251 231
EaeI y'GGCC_r
Cuts at: 0 75 1065 1269
Size: 75 990 204
Fragments arranged by size:
990 204 75
EarI CTCTTCn'nnn_
Cuts at: 0 46 98 1269
Size: 46 52 1171
Fragments arranged by size:
1171 52 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1118 1269
Size: 1118 151
EcoRI G'AATT_C
Cuts at: 0 263 1269
Size: 263 1006
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 539 571 982 1014 1269
Size: 539 32 411 32 255
Fragments arranged by size:
539 411 255 32 32
HincII GTy'rAC
Cuts at: 0 231 1269
Size: 231 1038
HindIII A'AGCT_T
Cuts at: 0 4 1220 1269
Size: 4 1216 49
Fragments arranged by size:
1216 49 4
MfeI C'AATT_G
Cuts at: 0 1148 1269
Size: 1148 121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 1269
Size: 344 925
MscI TGG'CCA
Cuts at: 0 77 1067 1269
Size: 77 990 202
Fragments arranged by size:
990 202 77
MslI CAynn'nnrTG
Cuts at: 0 251 791 922 1067 1269
Size: 251 540 131 145 202
Fragments arranged by size:
540 251 202 145 131
MspA1I CmG'CkG
Cuts at: 0 175 1231 1269
Size: 175 1056 38
Fragments arranged by size:
1056 175 38
NcoI C'CATG_G
Cuts at: 0 1068 1269
Size: 1068 201
NdeI CA'TA_TG
Cuts at: 0 144 520 1269
Size: 144 376 749
Fragments arranged by size:
749 376 144
NsiI A_TGCA'T
Cuts at: 0 222 929 1269
Size: 222 707 340
Fragments arranged by size:
707 340 222
NspI r_CATG'y
Cuts at: 0 58 927 1255 1269
Size: 58 869 328 14
Fragments arranged by size:
869 328 58 14
PflMI CCAn_nnn'nTGG
Cuts at: 0 551 788 1269
Size: 551 237 481
Fragments arranged by size:
551 481 237
PfoI T'CCnGG_A
Cuts at: 0 1095 1269
Size: 1095 174
PsiI TTA'TAA
Cuts at: 0 406 1269
Size: 406 863
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 20 52 1269
Size: 20 32 1217
Fragments arranged by size:
1217 32 20
PvuII CAG'CTG
Cuts at: 0 175 1269
Size: 175 1094
SalI G'TCGA_C
Cuts at: 0 229 1269
Size: 229 1040
SapI GCTCTTCn'nnn_
Cuts at: 0 98 1269
Size: 98 1171
SexAI A'CCwGG_T
Cuts at: 0 1051 1269
Size: 1051 218
SfcI C'TryA_G
Cuts at: 0 124 1212 1269
Size: 124 1088 57
Fragments arranged by size:
1088 124 57
SmlI C'TyrA_G
Cuts at: 0 885 1269
Size: 885 384
SpeI A'CTAG_T
Cuts at: 0 1039 1269
Size: 1039 230
SphI G_CATG'C
Cuts at: 0 927 1269
Size: 927 342
SspI AAT'ATT
Cuts at: 0 1138 1269
Size: 1138 131
StyI C'CwwG_G
Cuts at: 0 1068 1269
Size: 1068 201
SwaI ATTT'AAAT
Cuts at: 0 251 1269
Size: 251 1018
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 433 465 516 548 1269
Size: 433 32 51 32 721
Fragments arranged by size:
721 433 51 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 328 1269
Size: 328 941
XmnI GAAnn'nnTTC
Cuts at: 0 1019 1269
Size: 1019 250
Enzymes that do cut:
AccI AleI AlfI AloI ApoI AvaI BamHI BbsI
BfrBI BglII Bme1580I BmgBI BpmI BpuEI BseYI BsmI
Bsp1286I BsrBI BsrDI BsrFI BstAPI BstYI BstZ17I Bsu36I
BtgI BtsI DraI EaeI EarI Eco57MI EcoRI Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI
NdeI NsiI NspI PflMI PfoI PsiI PsrI PvuII
SalI SapI SexAI SfcI SmlI SpeI SphI SspI
StyI SwaI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BlpI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrGI BssHII
BssSI BstBI BstEII BstXI BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI RsrII SacI SacII SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI StuI
TaqII TatI XbaI XcmI XhoI XmaI ZraI