Restriction map for
pFN21AE1482
(Linear) (Six-base) MAPSORT of: KIEE1482.seq Check: 4343 from: 1 to: 2490
KIEE1482.orf
With 182 enzymes: SgfI *
July 15, 2010 18:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 79 502 867 1970 2490
Size: 79 423 365 1103 520
Fragments arranged by size:
1103 520 423 365 79
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 1305 1377 2490
Size: 576 729 72 1113
Fragments arranged by size:
1113 729 576 72
AflIII A'CryG_T
Cuts at: 0 1835 2490
Size: 1835 655
AgeI A'CCGG_T
Cuts at: 0 1513 2490
Size: 1513 977
AleI CACnn'nnGTG
Cuts at: 0 73 1906 2490
Size: 73 1833 584
Fragments arranged by size:
1833 584 73
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1885 1972 2490
Size: 1885 87 518
Fragments arranged by size:
1885 518 87
AlwNI CAG_nnn'CTG
Cuts at: 0 61 321 351 492 612 777 798 864
Size: 61 260 30 141 120 165 21 66
Cuts at: 864 2490
Size: 1626
Fragments arranged by size:
1626 260 165 141 120 66 61 30
21
ApaI G_GGCC'C
Cuts at: 0 360 768 2217 2490
Size: 360 408 1449 273
Fragments arranged by size:
1449 408 360 273
ApaLI G'TGCA_C
Cuts at: 0 1630 1672 2490
Size: 1630 42 818
Fragments arranged by size:
1630 818 42
ApoI r'AATT_y
Cuts at: 0 2419 2490
Size: 2419 71
AvaI C'yCGr_G
Cuts at: 0 272 472 760 811 1419 2490
Size: 272 200 288 51 608 1071
Fragments arranged by size:
1071 608 288 272 200 51
BamHI G'GATC_C
Cuts at: 0 1500 2490
Size: 1500 990
BanI G'GyrC_C
Cuts at: 0 261 514 1688 1929 2490
Size: 261 253 1174 241 561
Fragments arranged by size:
1174 561 261 253 241
BanII G_rGCy'C
Cuts at: 0 7 360 527 768 1721 2071 2120 2217
Size: 7 353 167 241 953 350 49 97
Cuts at: 2217 2490
Size: 273
Fragments arranged by size:
953 353 350 273 241 167 97 49
7
BbsI GAAGACnn'nnnn_
Cuts at: 0 193 720 1512 1653 2490
Size: 193 527 792 141 837
Fragments arranged by size:
837 792 527 193 141
BbvCI CC'TCA_GC
Cuts at: 0 413 2490
Size: 413 2077
BfrBI ATG'CAT
Cuts at: 0 1468 2490
Size: 1468 1022
BglI GCCn_nnn'nGGC
Cuts at: 0 355 873 1441 1804 2490
Size: 355 518 568 363 686
Fragments arranged by size:
686 568 518 363 355
BglII A'GATC_T
Cuts at: 0 1304 2490
Size: 1304 1186
Bme1580I G_kGCm'C
Cuts at: 0 360 768 1634 1676 1934 2217 2490
Size: 360 408 866 42 258 283 273
Fragments arranged by size:
866 408 360 283 273 258 42
BmgBI CAC'GTC
Cuts at: 0 726 2490
Size: 726 1764
BmrI ACTGGGnnnn_n'
Cuts at: 0 678 2421 2490
Size: 678 1743 69
Fragments arranged by size:
1743 678 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2397 2490
Size: 2397 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 448 579 622 634 1446 1479 1723
Size: 202 246 131 43 12 812 33 244
Cuts at: 1723 2085 2490
Size: 362 405
Fragments arranged by size:
812 405 362 246 244 202 131 43
33 12
Bpu10I CC'TnA_GC
Cuts at: 0 413 832 2218 2490
Size: 413 419 1386 272
Fragments arranged by size:
1386 419 413 272
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1599 2490
Size: 1599 891
BsaI GGTCTCn'nnnn_
Cuts at: 0 107 974 2490
Size: 107 867 1516
Fragments arranged by size:
1516 867 107
BsaAI yAC'GTr
Cuts at: 0 644 1671 2490
Size: 644 1027 819
Fragments arranged by size:
1027 819 644
BsaHI Gr'CG_yC
Cuts at: 0 454 1625 2490
Size: 454 1171 865
Fragments arranged by size:
1171 865 454
BsaWI w'CCGG_w
Cuts at: 0 14 1513 2490
Size: 14 1499 977
Fragments arranged by size:
1499 977 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2179 2209 2490
Size: 2179 30 281
Fragments arranged by size:
2179 281 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 190 223 310 763 1039 2007 2122
Size: 37 153 33 87 453 276 968 115
Cuts at: 2122 2490
Size: 368
Fragments arranged by size:
968 453 368 276 153 115 87 37
33
BseYI C'CCAG_C
Cuts at: 0 84 93 223 359 1478 1731 1810 1858
Size: 84 9 130 136 1119 253 79 48
Cuts at: 1858 2234 2490
Size: 376 256
Fragments arranged by size:
1119 376 256 253 136 130 84 79
48 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 901 945 1942 1951 2377 2392 2490
Size: 349 552 44 997 9 426 15 98
Fragments arranged by size:
997 552 426 349 98 44 15 9
BsiEI CG_ry'CG
Cuts at: 0 1279 2490
Size: 1279 1211
BsiHKAI G_wGCw'C
Cuts at: 0 620 937 973 1634 1676 1721 2071 2120
Size: 620 317 36 661 42 45 350 49
Cuts at: 2120 2138 2490
Size: 18 352
Fragments arranged by size:
661 620 352 350 317 49 45 42
36 18
BsmI GAATG_Cn'
Cuts at: 0 1468 2490
Size: 1468 1022
BsmBI CGTCTCn'nnnn_
Cuts at: 0 138 292 1699 2187 2490
Size: 138 154 1407 488 303
Fragments arranged by size:
1407 488 303 154 138
Bsp1286I G_dGCh'C
Cuts at: 0 7 360 527 620 768 937 973 1634
Size: 7 353 167 93 148 169 36 661
Cuts at: 1634 1676 1721 1934 2071 2120 2138 2217 2490
Size: 42 45 213 137 49 18 79 273
Fragments arranged by size:
661 353 273 213 169 167 148 137
93 79 49 45 42 36 18 7
BspEI T'CCGG_A
Cuts at: 0 14 2490
Size: 14 2476
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 79 287 502 867 873 992 1343 1970
Size: 79 208 215 365 6 119 351 627
Cuts at: 1970 2048 2210 2490
Size: 78 162 280
Fragments arranged by size:
627 365 351 280 215 208 162 119
79 78 6
BsrBI CCG'CTC
Cuts at: 0 825 1165 2490
Size: 825 340 1325
Fragments arranged by size:
1325 825 340
BsrDI GCAATG_nn'
Cuts at: 0 581 953 2490
Size: 581 372 1537
Fragments arranged by size:
1537 581 372
BsrFI r'CCGG_y
Cuts at: 0 258 1278 1369 1513 1691 2490
Size: 258 1020 91 144 178 799
Fragments arranged by size:
1020 799 258 178 144 91
BssSI C'ACGA_G
Cuts at: 0 100 1750 2490
Size: 100 1650 740
Fragments arranged by size:
1650 740 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 351 372 777 864 1058 1488 1695 1965
Size: 351 21 405 87 194 430 207 270
Cuts at: 1965 2400 2490
Size: 435 90
Fragments arranged by size:
435 430 405 351 270 207 194 90
87 21
BstEII G'GTnAC_C
Cuts at: 0 1744 2490
Size: 1744 746
BstXI CCAn_nnnn'nTGG
Cuts at: 0 436 644 1908 2490
Size: 436 208 1264 582
Fragments arranged by size:
1264 582 436 208
BstYI r'GATC_y
Cuts at: 0 692 1304 1500 2490
Size: 692 612 196 990
Fragments arranged by size:
990 692 612 196
Bsu36I CC'TnA_GG
Cuts at: 0 493 2490
Size: 493 1997
BtgI C'CryG_G
Cuts at: 0 518 570 1040 1224 1256 1439 1925 2258
Size: 518 52 470 184 32 183 486 333
Cuts at: 2258 2351 2490
Size: 93 139
Fragments arranged by size:
518 486 470 333 184 183 139 93
52 32
BtsI GCAGTG_nn'
Cuts at: 0 717 2490
Size: 717 1773
ClaI AT'CG_AT
Cuts at: 0 1547 2490
Size: 1547 943
DraIII CAC_nnn'GTG
Cuts at: 0 624 2415 2490
Size: 624 1791 75
Fragments arranged by size:
1791 624 75
DrdI GACnn_nn'nnGTC
Cuts at: 0 496 2490
Size: 496 1994
EaeI y'GGCC_r
Cuts at: 0 1103 1253 1433 1442 1680 1922 2490
Size: 1103 150 180 9 238 242 568
Fragments arranged by size:
1103 568 242 238 180 150 9
EarI CTCTTCn'nnn_
Cuts at: 0 1249 1744 1849 2099 2490
Size: 1249 495 105 250 391
Fragments arranged by size:
1249 495 391 250 105
EcoICRI GAG'CTC
Cuts at: 0 1719 2069 2118 2490
Size: 1719 350 49 372
Fragments arranged by size:
1719 372 350 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 448 576 579 622 634 1305 1377
Size: 202 246 128 3 43 12 671 72
Cuts at: 1377 1446 1479 1723 2085 2490
Size: 69 33 244 362 405
Fragments arranged by size:
671 405 362 246 244 202 128 72
69 43 33 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 205 313 447 463 835 997 2490
Size: 205 108 134 16 372 162 1493
Fragments arranged by size:
1493 372 205 162 134 108 16
EcoO109I rG'GnC_Cy
Cuts at: 0 276 348 440 468 489 534 563 764
Size: 276 72 92 28 21 45 29 201
Cuts at: 764 765 807 1002 1528 2091 2214 2490
Size: 1 42 195 526 563 123 276
Fragments arranged by size:
563 526 276 276 201 195 123 92
72 45 42 29 28 21 1
EcoRI G'AATT_C
Cuts at: 0 2419 2490
Size: 2419 71
FspI TGC'GCA
Cuts at: 0 286 2269 2490
Size: 286 1983 221
Fragments arranged by size:
1983 286 221
FspAI rTGC'GCAy
Cuts at: 0 2269 2490
Size: 2269 221
HaeII r_GCGC'y
Cuts at: 0 137 171 309 552 2490
Size: 137 34 138 243 1938
Fragments arranged by size:
1938 243 138 137 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 427 459 677 709 2490
Size: 427 32 218 32 1781
Fragments arranged by size:
1781 427 218 32 32
HincII GTy'rAC
Cuts at: 0 501 1158 2490
Size: 501 657 1332
Fragments arranged by size:
1332 657 501
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2210 2490
Size: 2210 280
MscI TGG'CCA
Cuts at: 0 1255 1444 1682 1924 2490
Size: 1255 189 238 242 566
Fragments arranged by size:
1255 566 242 238 189
MslI CAynn'nnrTG
Cuts at: 0 73 434 646 1834 1906 2263 2490
Size: 73 361 212 1188 72 357 227
Fragments arranged by size:
1188 361 357 227 212 73 72
MspA1I CmG'CkG
Cuts at: 0 58 363 372 417 572 612 777 861
Size: 58 305 9 45 155 40 165 84
Cuts at: 861 2002 2238 2490
Size: 1141 236 252
Fragments arranged by size:
1141 305 252 236 165 155 84 58
45 40 9
NaeI GCC'GGC
Cuts at: 0 260 2490
Size: 260 2230
NcoI C'CATG_G
Cuts at: 0 1256 1439 1925 2258 2490
Size: 1256 183 486 333 232
Fragments arranged by size:
1256 486 333 232 183
NgoMIV G'CCGG_C
Cuts at: 0 258 2490
Size: 258 2232
NsiI A_TGCA'T
Cuts at: 0 1470 2490
Size: 1470 1020
NspI r_CATG'y
Cuts at: 0 1071 1839 2490
Size: 1071 768 651
Fragments arranged by size:
1071 768 651
PciI A'CATG_T
Cuts at: 0 1835 2490
Size: 1835 655
PfoI T'CCnGG_A
Cuts at: 0 290 2490
Size: 290 2200
PmlI CAC'GTG
Cuts at: 0 644 1671 2490
Size: 644 1027 819
Fragments arranged by size:
1027 819 644
PpuMI rG'GwC_Cy
Cuts at: 0 276 489 807 1528 2490
Size: 276 213 318 721 962
Fragments arranged by size:
962 721 318 276 213
PspOMI G'GGCC_C
Cuts at: 0 356 764 2213 2490
Size: 356 408 1449 277
Fragments arranged by size:
1449 408 356 277
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2419 2451 2490
Size: 2419 32 39
Fragments arranged by size:
2419 39 32
PstI C_TGCA'G
Cuts at: 0 206 377 743 773 803 860 998 2490
Size: 206 171 366 30 30 57 138 1492
Fragments arranged by size:
1492 366 206 171 138 57 30 30
PvuII CAG'CTG
Cuts at: 0 363 372 417 777 861 2238 2490
Size: 363 9 45 360 84 1377 252
Fragments arranged by size:
1377 363 360 252 84 45 9
SacI G_AGCT'C
Cuts at: 0 1721 2071 2120 2490
Size: 1721 350 49 370
Fragments arranged by size:
1721 370 350 49
SacII CC_GC'GG
Cuts at: 0 573 2490
Size: 573 1917
SanDI GG'GwC_CC
Cuts at: 0 1528 2490
Size: 1528 962
SbfI CC_TGCA'GG
Cuts at: 0 206 998 2490
Size: 206 792 1492
Fragments arranged by size:
1492 792 206
SfcI C'TryA_G
Cuts at: 0 202 373 739 769 799 856 994 2490
Size: 202 171 366 30 30 57 138 1496
Fragments arranged by size:
1496 366 202 171 138 57 30 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 355 1441 1804 2490
Size: 355 1086 363 686
Fragments arranged by size:
1086 686 363 355
SgrAI Cr'CCGG_yG
Cuts at: 0 1513 2490
Size: 1513 977
SmaI CCC'GGG
Cuts at: 0 274 474 2490
Size: 274 200 2016
Fragments arranged by size:
2016 274 200
SmlI C'TyrA_G
Cuts at: 0 1578 2490
Size: 1578 912
StuI AGG'CCT
Cuts at: 0 964 2276 2490
Size: 964 1312 214
Fragments arranged by size:
1312 964 214
StyI C'CwwG_G
Cuts at: 0 1256 1439 1919 1925 2258 2490
Size: 1256 183 480 6 333 232
Fragments arranged by size:
1256 480 333 232 183 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2021 2403 2490
Size: 2021 382 87
Fragments arranged by size:
2021 382 87
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1675 2490
Size: 1675 815
XmaI C'CCGG_G
Cuts at: 0 272 472 2490
Size: 272 200 2018
Fragments arranged by size:
2018 272 200
XmnI GAAnn'nnTTC
Cuts at: 0 190 2431 2490
Size: 190 2241 59
Fragments arranged by size:
2241 190 59
Enzymes that do cut:
AarI AcuI AflIII AgeI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI ClaI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NsiI NspI PciI PfoI PmlI PpuMI
PspOMI PsrI PstI PvuII SacI SacII SanDI SbfI
SfcI SfiI SgrAI SmaI SmlI StuI StyI TaqII
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AhdI AloI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BclI BlpI BmtI BsaBI BsiWI BspHI BsrGI BssHII
BstBI BstZ17I BtgZI CspCI DraI EagI EciI EcoRV
FalI FseI HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI PacI PasI PflMI
PmeI PpiI PshAI PsiI PspXI PvuI RsrII SalI
SapI ScaI SexAI SfoI SgfI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI
ZraI