(Linear) (Six-base) MAPSORT of: KIEE1480.seq  Check: 1313  from: 1  to: 291



KIEE1480.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:39  ..

AfeI AGC'GCT

Cuts at:      0     152     291 
   Size:        152     139
 
AvaI C'yCGr_G

Cuts at:      0     105     291 
   Size:        105     186
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     281     291 
   Size:        281      10
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      28     291 
   Size:         28     263
 
BsaWI w'CCGG_w

Cuts at:      0      28     291 
   Size:         28     263
 
BspEI T'CCGG_A

Cuts at:      0      28     291 
   Size:         28     263
 
BsrDI GCAATG_nn'

Cuts at:      0     290     291 
   Size:        290       1
 
BssSI C'ACGA_G

Cuts at:      0      55     291 
   Size:         55     236
 
EaeI y'GGCC_r

Cuts at:      0      74     134     291 
   Size:         74      60     157

  Fragments arranged by size: 

                 157      74      60
 
HaeII r_GCGC'y

Cuts at:      0     154     291 
   Size:        154     137
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      23      55     149     181     291 
   Size:         23      32      94      32     110

  Fragments arranged by size: 

                 110      94      32      32      23
 
MscI TGG'CCA

Cuts at:      0     136     291 
   Size:        136     155
 
MspA1I CmG'CkG

Cuts at:      0     124     291 
   Size:        124     167
 
SmaI CCC'GGG

Cuts at:      0     107     291 
   Size:        107     184
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      55     291 
   Size:         55     236
 
XmaI C'CCGG_G

Cuts at:      0     105     291 
   Size:        105     186
 
XmnI GAAnn'nnTTC

Cuts at:      0     254     291 
   Size:        254      37
 

 Enzymes that do cut:

     AfeI     AvaI     BbsI     BmrI    BsaWI    BspEI    BsrDI    BssSI
     EaeI    HaeII    Hin4I     MscI   MspA1I     SmaI    TaqII     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     ZraI