Restriction map for
pFN21AE1479
(Linear) (Six-base) MAPSORT of: KIEE1479.seq Check: 7887 from: 1 to: 1773
KIEE1479.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 269 592 1387 1773
Size: 269 323 795 386
Fragments arranged by size:
795 386 323 269
AccI GT'mk_AC
Cuts at: 0 827 1400 1773
Size: 827 573 373
Fragments arranged by size:
827 573 373
AfeI AGC'GCT
Cuts at: 0 1190 1773
Size: 1190 583
AflIII A'CryG_T
Cuts at: 0 188 1773
Size: 188 1585
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 657 1773
Size: 657 1116
AlwNI CAG_nnn'CTG
Cuts at: 0 45 1617 1773
Size: 45 1572 156
Fragments arranged by size:
1572 156 45
ApaI G_GGCC'C
Cuts at: 0 562 650 1472 1650 1773
Size: 562 88 822 178 123
Fragments arranged by size:
822 562 178 123 88
ApaLI G'TGCA_C
Cuts at: 0 297 1773
Size: 297 1476
ApoI r'AATT_y
Cuts at: 0 1538 1773
Size: 1538 235
AscI GG'CGCG_CC
Cuts at: 0 352 1773
Size: 352 1421
AvaI C'yCGr_G
Cuts at: 0 466 561 787 1649 1672 1773
Size: 466 95 226 862 23 101
Fragments arranged by size:
862 466 226 101 95 23
BanI G'GyrC_C
Cuts at: 0 9 462 525 580 1209 1303 1368 1427
Size: 9 453 63 55 629 94 65 59
Cuts at: 1427 1509 1773
Size: 82 264
Fragments arranged by size:
629 453 264 94 82 65 63 59
55 9
BanII G_rGCy'C
Cuts at: 0 497 562 650 770 1472 1507 1591 1650
Size: 497 65 88 120 702 35 84 59
Cuts at: 1650 1773
Size: 123
Fragments arranged by size:
702 497 123 120 88 84 65 59
35
BbeI G_GCGC'C
Cuts at: 0 13 466 529 1431 1773
Size: 13 453 63 902 342
Fragments arranged by size:
902 453 342 63 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 939 1300 1341 1518 1773
Size: 939 361 41 177 255
Fragments arranged by size:
939 361 255 177 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 190 224 1773
Size: 190 34 1549
Fragments arranged by size:
1549 190 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1054 1773
Size: 1054 719
Bme1580I G_kGCm'C
Cuts at: 0 301 562 650 1214 1373 1472 1514 1650
Size: 301 261 88 564 159 99 42 136
Cuts at: 1650 1773
Size: 123
Fragments arranged by size:
564 301 261 159 136 123 99 88
42
BmgBI CAC'GTC
Cuts at: 0 191 1687 1773
Size: 191 1496 86
Fragments arranged by size:
1496 191 86
BmrI ACTGGGnnnn_n'
Cuts at: 0 1163 1773
Size: 1163 610
BmtI G_CTAG'C
Cuts at: 0 220 1773
Size: 220 1553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 784 943 1342 1773
Size: 88 696 159 399 431
Fragments arranged by size:
696 431 399 159 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1087 1773
Size: 1087 686
BsaI GGTCTCn'nnnn_
Cuts at: 0 315 545 1773
Size: 315 230 1228
Fragments arranged by size:
1228 315 230
BsaAI yAC'GTr
Cuts at: 0 612 906 1167 1773
Size: 612 294 261 606
Fragments arranged by size:
612 606 294 261
BsaHI Gr'CG_yC
Cuts at: 0 10 316 463 526 653 1428 1574 1773
Size: 10 306 147 63 127 775 146 199
Fragments arranged by size:
775 306 199 147 146 127 63 10
BseYI C'CCAG_C
Cuts at: 0 180 1233 1773
Size: 180 1053 540
Fragments arranged by size:
1053 540 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 921 1773
Size: 259 662 852
Fragments arranged by size:
852 662 259
BsiEI CG_ry'CG
Cuts at: 0 78 482 1463 1773
Size: 78 404 981 310
Fragments arranged by size:
981 404 310 78
BsiHKAI G_wGCw'C
Cuts at: 0 280 301 752 1591 1773
Size: 280 21 451 839 182
Fragments arranged by size:
839 451 280 182 21
Bsp1286I G_dGCh'C
Cuts at: 0 280 301 497 562 650 752 770 1214
Size: 280 21 196 65 88 102 18 444
Cuts at: 1214 1373 1472 1507 1514 1591 1650 1773
Size: 159 99 35 7 77 59 123
Fragments arranged by size:
444 280 196 159 123 102 99 88
77 65 59 35 21 18 7
BspHI T'CATG_A
Cuts at: 0 1289 1773
Size: 1289 484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 185 269 592 1387 1773
Size: 185 84 323 795 386
Fragments arranged by size:
795 386 323 185 84
BsrBI CCG'CTC
Cuts at: 0 626 1773
Size: 626 1147
BsrFI r'CCGG_y
Cuts at: 0 332 481 1255 1773
Size: 332 149 774 518
Fragments arranged by size:
774 518 332 149
BssHII G'CGCG_C
Cuts at: 0 352 365 538 896 1773
Size: 352 13 173 358 877
Fragments arranged by size:
877 358 352 173 13
BssSI C'ACGA_G
Cuts at: 0 627 1042 1773
Size: 627 415 731
Fragments arranged by size:
731 627 415
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1697 1773
Size: 1697 76
BstXI CCAn_nnnn'nTGG
Cuts at: 0 180 1773
Size: 180 1593
BstYI r'GATC_y
Cuts at: 0 128 1773
Size: 128 1645
BtgI C'CryG_G
Cuts at: 0 5 141 476 488 521 529 1059 1463
Size: 5 136 335 12 33 8 530 404
Cuts at: 1463 1517 1718 1773
Size: 54 201 55
Fragments arranged by size:
530 404 335 201 136 55 54 33
12 8 5
BtsI GCAGTG_nn'
Cuts at: 0 582 1695 1773
Size: 582 1113 78
Fragments arranged by size:
1113 582 78
EaeI y'GGCC_r
Cuts at: 0 2 75 397 479 704 857 974 1169
Size: 2 73 322 82 225 153 117 195
Cuts at: 1169 1460 1641 1773
Size: 291 181 132
Fragments arranged by size:
322 291 225 195 181 153 132 117
82 73 2
EagI C'GGCC_G
Cuts at: 0 75 479 1460 1773
Size: 75 404 981 313
Fragments arranged by size:
981 404 313 75
EarI CTCTTCn'nnn_
Cuts at: 0 613 1276 1773
Size: 613 663 497
Fragments arranged by size:
663 613 497
EcoICRI GAG'CTC
Cuts at: 0 1589 1773
Size: 1589 184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 784 943 1342 1773
Size: 88 696 159 399 431
Fragments arranged by size:
696 431 399 159 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1129 1773
Size: 1129 644
EcoO109I rG'GnC_Cy
Cuts at: 0 504 918 1089 1468 1773
Size: 504 414 171 379 305
Fragments arranged by size:
504 414 379 305 171
FseI GG_CCGG'CC
Cuts at: 0 485 1773
Size: 485 1288
FspI TGC'GCA
Cuts at: 0 633 1129 1455 1773
Size: 633 496 326 318
Fragments arranged by size:
633 496 326 318
FspAI rTGC'GCAy
Cuts at: 0 633 1773
Size: 633 1140
HaeII r_GCGC'y
Cuts at: 0 13 28 466 529 721 795 1192 1431
Size: 13 15 438 63 192 74 397 239
Cuts at: 1431 1773
Size: 342
Fragments arranged by size:
438 397 342 239 192 74 63 15
13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 1773
Size: 295 32 1446
Fragments arranged by size:
1446 295 32
KasI G'GCGC_C
Cuts at: 0 9 462 525 1427 1773
Size: 9 453 63 902 346
Fragments arranged by size:
902 453 346 63 9
MscI TGG'CCA
Cuts at: 0 976 1171 1773
Size: 976 195 602
Fragments arranged by size:
976 602 195
MspA1I CmG'CkG
Cuts at: 0 42 143 234 490 531 1459 1465 1639
Size: 42 101 91 256 41 928 6 174
Cuts at: 1639 1773
Size: 134
Fragments arranged by size:
928 256 174 134 101 91 42 41
6
NaeI GCC'GGC
Cuts at: 0 334 483 1773
Size: 334 149 1290
Fragments arranged by size:
1290 334 149
NarI GG'CG_CC
Cuts at: 0 10 463 526 1428 1773
Size: 10 453 63 902 345
Fragments arranged by size:
902 453 345 63 10
NgoMIV G'CCGG_C
Cuts at: 0 332 481 1773
Size: 332 149 1292
Fragments arranged by size:
1292 332 149
NheI G'CTAG_C
Cuts at: 0 216 1773
Size: 216 1557
NotI GC'GGCC_GC
Cuts at: 0 1460 1773
Size: 1460 313
NspI r_CATG'y
Cuts at: 0 171 516 1773
Size: 171 345 1257
Fragments arranged by size:
1257 345 171
PflMI CCAn_nnn'nTGG
Cuts at: 0 123 612 1773
Size: 123 489 1161
Fragments arranged by size:
1161 489 123
PfoI T'CCnGG_A
Cuts at: 0 546 911 1773
Size: 546 365 862
Fragments arranged by size:
862 546 365
PshAI GACnn'nnGTC
Cuts at: 0 1111 1773
Size: 1111 662
PspOMI G'GGCC_C
Cuts at: 0 558 646 1468 1646 1773
Size: 558 88 822 178 127
Fragments arranged by size:
822 558 178 127 88
PvuII CAG'CTG
Cuts at: 0 42 1639 1773
Size: 42 1597 134
Fragments arranged by size:
1597 134 42
SacI G_AGCT'C
Cuts at: 0 1591 1773
Size: 1591 182
SacII CC_GC'GG
Cuts at: 0 144 491 532 1466 1773
Size: 144 347 41 934 307
Fragments arranged by size:
934 347 307 144 41
SapI GCTCTTCn'nnn_
Cuts at: 0 613 1773
Size: 613 1160
SexAI A'CCwGG_T
Cuts at: 0 305 1009 1226 1773
Size: 305 704 217 547
Fragments arranged by size:
704 547 305 217
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1054 1773
Size: 1054 719
SfoI GGC'GCC
Cuts at: 0 11 464 527 1429 1773
Size: 11 453 63 902 344
Fragments arranged by size:
902 453 344 63 11
SmaI CCC'GGG
Cuts at: 0 563 1651 1674 1773
Size: 563 1088 23 99
Fragments arranged by size:
1088 563 99 23
SmlI C'TyrA_G
Cuts at: 0 1102 1773
Size: 1102 671
SphI G_CATG'C
Cuts at: 0 171 516 1773
Size: 171 345 1257
Fragments arranged by size:
1257 345 171
SrfI GCCC'GGGC
Cuts at: 0 563 1651 1773
Size: 563 1088 122
Fragments arranged by size:
1088 563 122
StuI AGG'CCT
Cuts at: 0 1660 1773
Size: 1660 113
StyI C'CwwG_G
Cuts at: 0 1655 1773
Size: 1655 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 981 1569 1773
Size: 981 588 204
Fragments arranged by size:
981 588 204
Tth111I GACn'n_nGTC
Cuts at: 0 307 1773
Size: 307 1466
XmaI C'CCGG_G
Cuts at: 0 561 1649 1672 1773
Size: 561 1088 23 101
Fragments arranged by size:
1088 561 101 23
Enzymes that do cut:
AarI AccI AfeI AflIII AlfI AlwNI ApaI ApaLI
ApoI AscI AvaI BanI BanII BbeI BbsI BcgI
BglI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI
BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI
BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FseI FspI FspAI HaeII Hin4I KasI MscI
MspA1I NaeI NarI NgoMIV NheI NotI NspI PflMI
PfoI PshAI PspOMI PvuII SacI SacII SapI SexAI
SfiI SfoI SmaI SmlI SphI SrfI StuI StyI
TaqII Tth111I XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AgeI AhdI AleI
AloI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BglII BlpI BplI Bpu10I BsaBI BsaWI
BsaXI BseRI BsiWI BsmI BsmBI BspEI BsrDI BsrGI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EciI EcoRI EcoRV FalI HincII HindIII
HpaI KpnI MfeI MluI MmeI MslI NcoI NdeI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PpuMI PsiI PspXI PsrI PstI PvuI RsrII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SnaBI SpeI
SspI SwaI TatI TstI XbaI XcmI XhoI XmnI
ZraI