(Linear) (Six-base) MAPSORT of: KIEE1477.seq  Check: 9815  from: 1  to: 1053



KIEE1477.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:39  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     310     794    1053 
   Size:        170     140     484     259

  Fragments arranged by size: 

                 484     259     170     140
 
AleI CACnn'nnGTG

Cuts at:      0     522    1053 
   Size:        522     531
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     893     925    1053 
   Size:        893      32     128

  Fragments arranged by size: 

                 893     128      32
 
ApaLI G'TGCA_C

Cuts at:      0     555    1053 
   Size:        555     498
 
ApoI r'AATT_y

Cuts at:      0      16     547     800    1053 
   Size:         16     531     253     253

  Fragments arranged by size: 

                 531     253     253      16
 
AvaI C'yCGr_G

Cuts at:      0     992    1053 
   Size:        992      61
 
BanI G'GyrC_C

Cuts at:      0     103     873    1053 
   Size:        103     770     180

  Fragments arranged by size: 

                 770     180     103
 
BanII G_rGCy'C

Cuts at:      0     759    1053 
   Size:        759     294
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     298    1053 
   Size:        298     755
 
BclI T'GATC_A

Cuts at:      0     300    1053 
   Size:        300     753
 
BglII A'GATC_T

Cuts at:      0     401    1053 
   Size:        401     652
 
Bme1580I G_kGCm'C

Cuts at:      0     559     952    1053 
   Size:        559     393     101

  Fragments arranged by size: 

                 559     393     101
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     405     679    1053 
   Size:        405     274     374

  Fragments arranged by size: 

                 405     374     274
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     766    1053 
   Size:        766     287
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     393    1053 
   Size:        393     660
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     668     735     754     836    1053 
   Size:        668      67      19      82     217

  Fragments arranged by size: 

                 668     217      82      67      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83    1053 
   Size:         83     970
 
BsiHKAI G_wGCw'C

Cuts at:      0     559    1053 
   Size:        559     494
 
BsmI GAATG_Cn'

Cuts at:      0      90    1053 
   Size:         90     963
 
Bsp1286I G_dGCh'C

Cuts at:      0     559     759     952    1053 
   Size:        559     200     193     101

  Fragments arranged by size: 

                 559     200     193     101
 
BstYI r'GATC_y

Cuts at:      0     401    1053 
   Size:        401     652
 
BtgI C'CryG_G

Cuts at:      0    1014    1053 
   Size:       1014      39
 
BtsI GCAGTG_nn'

Cuts at:      0     682    1053 
   Size:        682     371
 
DraI TTT'AAA

Cuts at:      0     261    1053 
   Size:        261     792
 
EarI CTCTTCn'nnn_

Cuts at:      0     922    1053 
   Size:        922     131
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     310     766     794    1053 
   Size:        170     140     456      28     259

  Fragments arranged by size: 

                 456     259     170     140      28
 
EcoRV GAT'ATC

Cuts at:      0     309    1053 
   Size:        309     744
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     285     317    1053 
   Size:        285      32     736

  Fragments arranged by size: 

                 736     285      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     706    1053 
   Size:        332     374     347

  Fragments arranged by size: 

                 374     347     332
 
MslI CAynn'nnrTG

Cuts at:      0     522    1053 
   Size:        522     531
 
MspA1I CmG'CkG

Cuts at:      0     605    1053 
   Size:        605     448
 
NcoI C'CATG_G

Cuts at:      0    1014    1053 
   Size:       1014      39
 
NdeI CA'TA_TG

Cuts at:      0     646    1053 
   Size:        646     407
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     856    1053 
   Size:        856     197
 
PfoI T'CCnGG_A

Cuts at:      0     406     897    1053 
   Size:        406     491     156

  Fragments arranged by size: 

                 491     406     156
 
PspXI vC'TCGA_Gb

Cuts at:      0     992    1053 
   Size:        992      61
 
PvuII CAG'CTG

Cuts at:      0     605    1053 
   Size:        605     448
 
SexAI A'CCwGG_T

Cuts at:      0     855    1053 
   Size:        855     198
 
SmlI C'TyrA_G

Cuts at:      0     992    1053 
   Size:        992      61
 
SspI AAT'ATT

Cuts at:      0     362    1053 
   Size:        362     691
 
StuI AGG'CCT

Cuts at:      0     933    1053 
   Size:        933     120
 
StyI C'CwwG_G

Cuts at:      0      89     107    1014    1053 
   Size:         89      18     907      39

  Fragments arranged by size: 

                 907      89      39      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     310    1053 
   Size:        310     743
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     332     364    1053 
   Size:        332      32     689

  Fragments arranged by size: 

                 689     332      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     404    1053 
   Size:        404     649
 
XhoI C'TCGA_G

Cuts at:      0     992    1053 
   Size:        992      61
 

 Enzymes that do cut:

     AcuI     AleI     AloI    ApaLI     ApoI     AvaI     BanI    BanII
     BbsI     BclI    BglII Bme1580I     BplI     BpmI     BsaI    BseRI
     BsgI  BsiHKAI     BsmI Bsp1286I    BstYI     BtgI     BtsI     DraI
     EarI  Eco57MI    EcoRV    Hin4I     MmeI     MslI   MspA1I     NcoI
     NdeI    PflMI     PfoI    PspXI    PvuII    SexAI     SmlI     SspI
     StuI     StyI    TaqII     TstI     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI    AlwNI     ApaI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BglI     BlpI    BmgBI     BmrI     BmtI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI
     TatI  Tth111I     XbaI     XmaI     XmnI     ZraI