Restriction map for
pFN21AE1476
(Linear) (Six-base) MAPSORT of: KIEE1476.seq Check: 5278 from: 1 to: 1965
KIEE1476.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
Acc65I G'GTAC_C
Cuts at: 0 520 1965
Size: 520 1445
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 949 1201 1276 1965
Size: 868 81 252 75 689
Fragments arranged by size:
868 689 252 81 75
AlwNI CAG_nnn'CTG
Cuts at: 0 487 588 826 1965
Size: 487 101 238 1139
Fragments arranged by size:
1139 487 238 101
ApaI G_GGCC'C
Cuts at: 0 991 1142 1411 1965
Size: 991 151 269 554
Fragments arranged by size:
991 554 269 151
ApoI r'AATT_y
Cuts at: 0 1593 1965
Size: 1593 372
AvaI C'yCGr_G
Cuts at: 0 515 1119 1411 1557 1965
Size: 515 604 292 146 408
Fragments arranged by size:
604 515 408 292 146
BamHI G'GATC_C
Cuts at: 0 1149 1965
Size: 1149 816
BanI G'GyrC_C
Cuts at: 0 520 1965
Size: 520 1445
BanII G_rGCy'C
Cuts at: 0 85 991 1142 1411 1543 1564 1667 1965
Size: 85 906 151 269 132 21 103 298
Fragments arranged by size:
906 298 269 151 132 103 85 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 863 1502 1965
Size: 863 639 463
Fragments arranged by size:
863 639 463
BbvCI CC'TCA_GC
Cuts at: 0 1862 1965
Size: 1862 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 12 30 46 64 496 530 1965
Size: 12 18 16 18 432 34 1435
Fragments arranged by size:
1435 432 34 18 18 16 12
BciVI GTATCCnnnnn_n'
Cuts at: 0 1117 1965
Size: 1117 848
BclI T'GATC_A
Cuts at: 0 596 1965
Size: 596 1369
BglI GCCn_nnn'nGGC
Cuts at: 0 404 1965
Size: 404 1561
BlpI GC'TnA_GC
Cuts at: 0 122 840 1552 1708 1965
Size: 122 718 712 156 257
Fragments arranged by size:
718 712 257 156 122
Bme1580I G_kGCm'C
Cuts at: 0 435 991 1142 1411 1965
Size: 435 556 151 269 554
Fragments arranged by size:
556 554 435 269 151
BmgBI CAC'GTC
Cuts at: 0 861 1965
Size: 861 1104
BmrI ACTGGGnnnn_n'
Cuts at: 0 965 1148 1965
Size: 965 183 817
Fragments arranged by size:
965 817 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 1965
Size: 636 1329
Bpu10I CC'TnA_GC
Cuts at: 0 106 698 1862 1965
Size: 106 592 1164 103
Fragments arranged by size:
1164 592 106 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1609 1965
Size: 1609 356
BsaBI GATnn'nnATC
Cuts at: 0 1298 1965
Size: 1298 667
BsaWI w'CCGG_w
Cuts at: 0 1109 1454 1965
Size: 1109 345 511
Fragments arranged by size:
1109 511 345
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1514 1544 1965
Size: 1514 30 421
Fragments arranged by size:
1514 421 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1689 1843 1965
Size: 1689 154 122
Fragments arranged by size:
1689 154 122
BseYI C'CCAG_C
Cuts at: 0 1427 1505 1543 1570 1965
Size: 1427 78 38 27 395
Fragments arranged by size:
1427 395 78 38 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1122 1965
Size: 1122 843
BsiHKAI G_wGCw'C
Cuts at: 0 516 704 1965
Size: 516 188 1261
Fragments arranged by size:
1261 516 188
BsmI GAATG_Cn'
Cuts at: 0 394 1965
Size: 394 1571
Bsp1286I G_dGCh'C
Cuts at: 0 85 435 516 704 991 1142 1411 1543
Size: 85 350 81 188 287 151 269 132
Cuts at: 1543 1564 1667 1965
Size: 21 103 298
Fragments arranged by size:
350 298 287 269 188 151 132 103
85 81 21
BspEI T'CCGG_A
Cuts at: 0 1109 1965
Size: 1109 856
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1752 1965
Size: 1752 213
BsrBI CCG'CTC
Cuts at: 0 1371 1600 1965
Size: 1371 229 365
Fragments arranged by size:
1371 365 229
BsrDI GCAATG_nn'
Cuts at: 0 1239 1320 1965
Size: 1239 81 645
Fragments arranged by size:
1239 645 81
BsrFI r'CCGG_y
Cuts at: 0 181 1877 1965
Size: 181 1696 88
Fragments arranged by size:
1696 181 88
BssSI C'ACGA_G
Cuts at: 0 1366 1965
Size: 1366 599
BstAPI GCAn_nnn'nTGC
Cuts at: 0 269 1965
Size: 269 1696
BstBI TT'CG_AA
Cuts at: 0 1886 1965
Size: 1886 79
BstXI CCAn_nnnn'nTGG
Cuts at: 0 341 1509 1965
Size: 341 1168 456
Fragments arranged by size:
1168 456 341
BstYI r'GATC_y
Cuts at: 0 1149 1220 1965
Size: 1149 71 745
Fragments arranged by size:
1149 745 71
Bsu36I CC'TnA_GG
Cuts at: 0 1826 1965
Size: 1826 139
BtgI C'CryG_G
Cuts at: 0 340 944 1239 1965
Size: 340 604 295 726
Fragments arranged by size:
726 604 340 295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 33 1965
Size: 33 1932
BtsI GCAGTG_nn'
Cuts at: 0 514 1965
Size: 514 1451
ClaI AT'CG_AT
Cuts at: 0 1247 1965
Size: 1247 718
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 612 647 1965
Size: 612 35 1318
Fragments arranged by size:
1318 612 35
EaeI y'GGCC_r
Cuts at: 0 183 343 1236 1965
Size: 183 160 893 729
Fragments arranged by size:
893 729 183 160
EarI CTCTTCn'nnn_
Cuts at: 0 777 1841 1965
Size: 777 1064 124
Fragments arranged by size:
1064 777 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 868 949 1201 1276 1965
Size: 636 232 81 252 75 689
Fragments arranged by size:
689 636 252 232 81 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 288 1965
Size: 288 1677
EcoO109I rG'GnC_Cy
Cuts at: 0 5 281 907 987 988 1138 1139 1407
Size: 5 276 626 80 1 150 1 268
Cuts at: 1407 1408 1965
Size: 1 557
Fragments arranged by size:
626 557 276 268 150 80 5 1
1 1
EcoRI G'AATT_C
Cuts at: 0 1593 1965
Size: 1593 372
EcoRV GAT'ATC
Cuts at: 0 1251 1296 1965
Size: 1251 45 669
Fragments arranged by size:
1251 669 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1662 1965
Size: 1662 303
HaeII r_GCGC'y
Cuts at: 0 1442 1965
Size: 1442 523
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 987 1019 1672 1704 1965
Size: 987 32 653 32 261
Fragments arranged by size:
987 653 261 32 32
HincII GTy'rAC
Cuts at: 0 171 894 1965
Size: 171 723 1071
Fragments arranged by size:
1071 723 171
HindIII A'AGCT_T
Cuts at: 0 1258 1965
Size: 1258 707
KpnI G_GTAC'C
Cuts at: 0 524 1965
Size: 524 1441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 1222 1640 1965
Size: 791 431 418 325
Fragments arranged by size:
791 431 418 325
MscI TGG'CCA
Cuts at: 0 345 1238 1965
Size: 345 893 727
Fragments arranged by size:
893 727 345
MslI CAynn'nnrTG
Cuts at: 0 299 339 1965
Size: 299 40 1626
Fragments arranged by size:
1626 299 40
MspA1I CmG'CkG
Cuts at: 0 1509 1676 1694 1965
Size: 1509 167 18 271
Fragments arranged by size:
1509 271 167 18
NcoI C'CATG_G
Cuts at: 0 340 944 1239 1965
Size: 340 604 295 726
Fragments arranged by size:
726 604 340 295
NspI r_CATG'y
Cuts at: 0 411 1809 1965
Size: 411 1398 156
Fragments arranged by size:
1398 411 156
PfoI T'CCnGG_A
Cuts at: 0 809 1928 1965
Size: 809 1119 37
Fragments arranged by size:
1119 809 37
PpuMI rG'GwC_Cy
Cuts at: 0 5 281 1965
Size: 5 276 1684
Fragments arranged by size:
1684 276 5
PshAI GACnn'nnGTC
Cuts at: 0 206 326 1965
Size: 206 120 1639
Fragments arranged by size:
1639 206 120
PspOMI G'GGCC_C
Cuts at: 0 987 1138 1407 1965
Size: 987 151 269 558
Fragments arranged by size:
987 558 269 151
PspXI vC'TCGA_Gb
Cuts at: 0 1119 1965
Size: 1119 846
PstI C_TGCA'G
Cuts at: 0 43 265 1391 1965
Size: 43 222 1126 574
Fragments arranged by size:
1126 574 222 43
PvuII CAG'CTG
Cuts at: 0 1509 1965
Size: 1509 456
SexAI A'CCwGG_T
Cuts at: 0 587 1965
Size: 587 1378
SfcI C'TryA_G
Cuts at: 0 39 261 324 1387 1965
Size: 39 222 63 1063 578
Fragments arranged by size:
1063 578 222 63 39
SmlI C'TyrA_G
Cuts at: 0 1119 1588 1965
Size: 1119 469 377
Fragments arranged by size:
1119 469 377
StuI AGG'CCT
Cuts at: 0 139 1965
Size: 139 1826
StyI C'CwwG_G
Cuts at: 0 340 611 944 1239 1965
Size: 340 271 333 295 726
Fragments arranged by size:
726 340 333 295 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 398 1519 1965
Size: 398 1121 446
Fragments arranged by size:
1121 446 398
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 484 516 723 755 1965
Size: 484 32 207 32 1210
Fragments arranged by size:
1210 484 207 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 625 1965
Size: 625 1340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1161 1965
Size: 1161 804
XhoI C'TCGA_G
Cuts at: 0 1119 1965
Size: 1119 846
XmnI GAAnn'nnTTC
Cuts at: 0 1850 1965
Size: 1850 115
Enzymes that do cut:
Acc65I AcuI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I
NcoI NspI PfoI PpuMI PshAI PspOMI PspXI PstI
PvuII SexAI SfcI SmlI StuI StyI TaqII TstI
Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BfrBI BglII BmtI BplI BsaI
BsaAI BsaHI BsiEI BsiWI BsmBI BspHI BsrGI BssHII
BstEII BstZ17I DraI DraIII DrdI EagI EciI EcoICRI
FseI FspI FspAI HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PsiI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI XbaI XmaI ZraI