(Linear) (Six-base) MAPSORT of: KIEE1475.seq  Check: 1877  from: 1  to: 492



KIEE1475.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:39  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     480     492 
   Size:        480      12
 
BamHI G'GATC_C

Cuts at:      0      81     492 
   Size:         81     411
 
BanII G_rGCy'C

Cuts at:      0     224     492 
   Size:        224     268
 
BglI GCCn_nnn'nGGC

Cuts at:      0     401     492 
   Size:        401      91
 
Bme1580I G_kGCm'C

Cuts at:      0     413     492 
   Size:        413      79
 
BmgBI CAC'GTC

Cuts at:      0     268     492 
   Size:        268     224
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     436     492 
   Size:        436      56
 
BsaHI Gr'CG_yC

Cuts at:      0     179     461     492 
   Size:        179     282      31

  Fragments arranged by size: 

                 282     179      31
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     256     492 
   Size:        256     236
 
BsiHKAI G_wGCw'C

Cuts at:      0     224     492 
   Size:        224     268
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     258     492 
   Size:        258     234
 
Bsp1286I G_dGCh'C

Cuts at:      0     224     413     492 
   Size:        224     189      79

  Fragments arranged by size: 

                 224     189      79
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     107     492 
   Size:        107     385
 
BsrGI T'GTAC_A

Cuts at:      0     329     492 
   Size:        329     163
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     480     492 
   Size:        480      12
 
BstYI r'GATC_y

Cuts at:      0      81     492 
   Size:         81     411
 
Bsu36I CC'TnA_GG

Cuts at:      0     317     492 
   Size:        317     175
 
BtgI C'CryG_G

Cuts at:      0      23     492 
   Size:         23     469
 
BtsI GCAGTG_nn'

Cuts at:      0     137     492 
   Size:        137     355
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     249     284     492 
   Size:        249      35     208

  Fragments arranged by size: 

                 249     208      35
 
EaeI y'GGCC_r

Cuts at:      0      26     270     492 
   Size:         26     244     222

  Fragments arranged by size: 

                 244     222      26
 
EarI CTCTTCn'nnn_

Cuts at:      0     121     231     492 
   Size:        121     110     261

  Fragments arranged by size: 

                 261     121     110
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     373     492 
   Size:        373     119
 
EcoICRI GAG'CTC

Cuts at:      0     222     492 
   Size:        222     270
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     320     424     492 
   Size:        320     104      68

  Fragments arranged by size: 

                 320     104      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0     276     477     492 
   Size:        276     201      15

  Fragments arranged by size: 

                 276     201      15
 
HaeII r_GCGC'y

Cuts at:      0      16     396     492 
   Size:         16     380      96

  Fragments arranged by size: 

                 380      96      16
 
MspA1I CmG'CkG

Cuts at:      0      25     492 
   Size:         25     467
 
NspI r_CATG'y

Cuts at:      0     474     492 
   Size:        474      18
 
PpuMI rG'GwC_Cy

Cuts at:      0     276     492 
   Size:        276     216
 
PstI C_TGCA'G

Cuts at:      0      38     344     492 
   Size:         38     306     148

  Fragments arranged by size: 

                 306     148      38
 
SacI G_AGCT'C

Cuts at:      0     224     492 
   Size:        224     268
 
SacII CC_GC'GG

Cuts at:      0      26     492 
   Size:         26     466
 
SanDI GG'GwC_CC

Cuts at:      0     276     492 
   Size:        276     216
 
SbfI CC_TGCA'GG

Cuts at:      0      38     492 
   Size:         38     454
 
SfcI C'TryA_G

Cuts at:      0      34     340     366     492 
   Size:         34     306      26     126

  Fragments arranged by size: 

                 306     126      34      26
 
StuI AGG'CCT

Cuts at:      0     326     364     492 
   Size:        326      38     128

  Fragments arranged by size: 

                 326     128      38
 
StyI C'CwwG_G

Cuts at:      0     446     492 
   Size:        446      46
 
TatI w'GTAC_w

Cuts at:      0     329     436     492 
   Size:        329     107      56

  Fragments arranged by size: 

                 329     107      56
 

 Enzymes that do cut:

    AlwNI    BamHI    BanII     BglI Bme1580I    BmgBI     BsaI    BsaHI
    BseRI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrGI   BstAPI    BstYI
   Bsu36I     BtgI     BtsI    CspCI     EaeI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    HaeII   MspA1I     NspI    PpuMI     PstI     SacI
    SacII    SanDI     SbfI     SfcI     StuI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     ClaI     DraI   DraIII     DrdI
     EagI  Eco57MI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SalI     SapI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI