Restriction map for
pFN21AE1474
(Linear) (Six-base) MAPSORT of: KIEE1474.seq Check: 2268 from: 1 to: 1506
KIEE1474.orf
With 182 enzymes: SgfI *
July 3, 2012 16:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1443 1506
Size: 1443 63
AatII G_ACGT'C
Cuts at: 0 1199 1506
Size: 1199 307
AccI GT'mk_AC
Cuts at: 0 1337 1506
Size: 1337 169
Acc65I G'GTAC_C
Cuts at: 0 1216 1506
Size: 1216 290
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 1506
Size: 683 823
AleI CACnn'nnGTG
Cuts at: 0 907 1506
Size: 907 599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1160 1506
Size: 1160 346
AlwNI CAG_nnn'CTG
Cuts at: 0 718 779 1385 1506
Size: 718 61 606 121
Fragments arranged by size:
718 606 121 61
ApaI G_GGCC'C
Cuts at: 0 414 1506
Size: 414 1092
ApoI r'AATT_y
Cuts at: 0 465 1057 1506
Size: 465 592 449
Fragments arranged by size:
592 465 449
AvaI C'yCGr_G
Cuts at: 0 50 123 1506
Size: 50 73 1383
Fragments arranged by size:
1383 73 50
BamHI G'GATC_C
Cuts at: 0 540 1506
Size: 540 966
BanI G'GyrC_C
Cuts at: 0 1216 1506
Size: 1216 290
BanII G_rGCy'C
Cuts at: 0 414 1506
Size: 414 1092
BbsI GAAGACnn'nnnn_
Cuts at: 0 964 1170 1506
Size: 964 206 336
Fragments arranged by size:
964 336 206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 630 664 1354 1388 1506
Size: 630 34 690 34 118
Fragments arranged by size:
690 630 118 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1490 1506
Size: 1490 16
BlpI GC'TnA_GC
Cuts at: 0 16 1506
Size: 16 1490
Bme1580I G_kGCm'C
Cuts at: 0 414 1506
Size: 414 1092
BmrI ACTGGGnnnn_n'
Cuts at: 0 92 728 1238 1506
Size: 92 636 510 268
Fragments arranged by size:
636 510 268 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 1506
Size: 77 1429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 1241 1506
Size: 153 1088 265
Fragments arranged by size:
1088 265 153
BsaAI yAC'GTr
Cuts at: 0 1329 1506
Size: 1329 177
BsaHI Gr'CG_yC
Cuts at: 0 824 1196 1506
Size: 824 372 310
Fragments arranged by size:
824 372 310
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 585 615 1506
Size: 585 30 891
Fragments arranged by size:
891 585 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 586 868 1506
Size: 586 282 638
Fragments arranged by size:
638 586 282
BseYI C'CCAG_C
Cuts at: 0 206 1506
Size: 206 1300
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 763 1156 1303 1448 1506
Size: 54 709 393 147 145 58
Fragments arranged by size:
709 393 147 145 58 54
BsiEI CG_ry'CG
Cuts at: 0 62 1506
Size: 62 1444
BsiHKAI G_wGCw'C
Cuts at: 0 809 1506
Size: 809 697
Bsp1286I G_dGCh'C
Cuts at: 0 414 809 1506
Size: 414 395 697
Fragments arranged by size:
697 414 395
BspHI T'CATG_A
Cuts at: 0 1403 1506
Size: 1403 103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1443 1506
Size: 1443 63
BsrDI GCAATG_nn'
Cuts at: 0 1071 1506
Size: 1071 435
BssSI C'ACGA_G
Cuts at: 0 7 1506
Size: 7 1499
BstAPI GCAn_nnn'nTGC
Cuts at: 0 779 1506
Size: 779 727
BstXI CCAn_nnnn'nTGG
Cuts at: 0 606 1139 1506
Size: 606 533 367
Fragments arranged by size:
606 533 367
BstYI r'GATC_y
Cuts at: 0 540 923 1506
Size: 540 383 583
Fragments arranged by size:
583 540 383
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1226 1506
Size: 1226 280
BtsI GCAGTG_nn'
Cuts at: 0 551 1144 1299 1415 1506
Size: 551 593 155 116 91
Fragments arranged by size:
593 551 155 116 91
DrdI GACnn_nn'nnGTC
Cuts at: 0 1174 1506
Size: 1174 332
EaeI y'GGCC_r
Cuts at: 0 23 899 1506
Size: 23 876 607
Fragments arranged by size:
876 607 23
EarI CTCTTCn'nnn_
Cuts at: 0 1270 1506
Size: 1270 236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 683 1506
Size: 77 606 823
Fragments arranged by size:
823 606 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1405 1506
Size: 1405 101
EcoO109I rG'GnC_Cy
Cuts at: 0 138 410 1506
Size: 138 272 1096
Fragments arranged by size:
1096 272 138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 981 1506
Size: 981 525
HaeII r_GCGC'y
Cuts at: 0 44 1506
Size: 44 1462
HincII GTy'rAC
Cuts at: 0 1200 1506
Size: 1200 306
KpnI G_GTAC'C
Cuts at: 0 1220 1506
Size: 1220 286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 657 810 1506
Size: 657 153 696
Fragments arranged by size:
696 657 153
MscI TGG'CCA
Cuts at: 0 901 1506
Size: 901 605
MslI CAynn'nnrTG
Cuts at: 0 460 907 1506
Size: 460 447 599
Fragments arranged by size:
599 460 447
MspA1I CmG'CkG
Cuts at: 0 293 747 1506
Size: 293 454 759
Fragments arranged by size:
759 454 293
PasI CC'CwG_GG
Cuts at: 0 606 1506
Size: 606 900
PflMI CCAn_nnn'nTGG
Cuts at: 0 718 1506
Size: 718 788
PpuMI rG'GwC_Cy
Cuts at: 0 138 1506
Size: 138 1368
PspOMI G'GGCC_C
Cuts at: 0 410 1506
Size: 410 1096
PstI C_TGCA'G
Cuts at: 0 1294 1506
Size: 1294 212
PvuII CAG'CTG
Cuts at: 0 293 747 1506
Size: 293 454 759
Fragments arranged by size:
759 454 293
SfcI C'TryA_G
Cuts at: 0 288 380 1290 1506
Size: 288 92 910 216
Fragments arranged by size:
910 288 216 92
SmaI CCC'GGG
Cuts at: 0 125 1506
Size: 125 1381
SmlI C'TyrA_G
Cuts at: 0 132 1256 1506
Size: 132 1124 250
Fragments arranged by size:
1124 250 132
SpeI A'CTAG_T
Cuts at: 0 163 1506
Size: 163 1343
SspI AAT'ATT
Cuts at: 0 420 1506
Size: 420 1086
StuI AGG'CCT
Cuts at: 0 319 1506
Size: 319 1187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 897 1506
Size: 897 609
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 585 617 788 820 1506
Size: 585 32 171 32 686
Fragments arranged by size:
686 585 171 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1171 1506
Size: 1171 335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 95 909 1506
Size: 95 814 597
Fragments arranged by size:
814 597 95
XmaI C'CCGG_G
Cuts at: 0 123 1506
Size: 123 1383
XmnI GAAnn'nnTTC
Cuts at: 0 1061 1506
Size: 1061 445
ZraI GAC'GTC
Cuts at: 0 1197 1506
Size: 1197 309
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AleI AlfI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbsI BcgI
BglI BlpI Bme1580I BmrI BpmI BpuEI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BssSI BstAPI BstXI BstYI BtgZI BtsI
DrdI EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII
HincII KpnI MmeI MscI MslI MspA1I PasI PflMI
PpuMI PspOMI PstI PvuII SfcI SmaI SmlI SpeI
SspI StuI TaqII TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI
BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BsaI
BsaBI BsaWI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraI DraIII EagI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI Hin4I HindIII HpaI KasI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SphI SrfI StyI SwaI
TatI XbaI XhoI