Restriction map for
pFN21AE1472
(Linear) (Six-base) MAPSORT of: KIEE1472.seq Check: 4161 from: 1 to: 915
KIEE1472.orf
With 182 enzymes: SgfI *
July 15, 2010 18:39 ..
AccI GT'mk_AC
Cuts at: 0 161 789 915
Size: 161 628 126
Fragments arranged by size:
628 161 126
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 915
Size: 176 739
ApoI r'AATT_y
Cuts at: 0 85 175 868 915
Size: 85 90 693 47
Fragments arranged by size:
693 90 85 47
AseI AT'TA_AT
Cuts at: 0 658 915
Size: 658 257
AvrII C'CTAG_G
Cuts at: 0 904 915
Size: 904 11
BanII G_rGCy'C
Cuts at: 0 34 213 915
Size: 34 179 702
Fragments arranged by size:
702 179 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 895 915
Size: 895 20
BclI T'GATC_A
Cuts at: 0 554 915
Size: 554 361
BfrBI ATG'CAT
Cuts at: 0 798 915
Size: 798 117
BlpI GC'TnA_GC
Cuts at: 0 53 915
Size: 53 862
BsaAI yAC'GTr
Cuts at: 0 165 915
Size: 165 750
BsaWI w'CCGG_w
Cuts at: 0 505 915
Size: 505 410
BseYI C'CCAG_C
Cuts at: 0 27 915
Size: 27 888
BsiHKAI G_wGCw'C
Cuts at: 0 213 915
Size: 213 702
BsmI GAATG_Cn'
Cuts at: 0 621 915
Size: 621 294
Bsp1286I G_dGCh'C
Cuts at: 0 34 213 915
Size: 34 179 702
Fragments arranged by size:
702 179 34
BsrDI GCAATG_nn'
Cuts at: 0 713 915
Size: 713 202
BstYI r'GATC_y
Cuts at: 0 502 915
Size: 502 413
BstZ17I GTA'TAC
Cuts at: 0 162 790 915
Size: 162 628 125
Fragments arranged by size:
628 162 125
DraI TTT'AAA
Cuts at: 0 180 915
Size: 180 735
DrdI GACnn_nn'nnGTC
Cuts at: 0 109 915
Size: 109 806
EarI CTCTTCn'nnn_
Cuts at: 0 180 812 915
Size: 180 632 103
Fragments arranged by size:
632 180 103
EcoICRI GAG'CTC
Cuts at: 0 211 915
Size: 211 704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 915
Size: 176 739
EcoRI G'AATT_C
Cuts at: 0 85 915
Size: 85 830
HaeII r_GCGC'y
Cuts at: 0 22 915
Size: 22 893
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 539 571 755 787 915
Size: 301 32 206 32 184 32 128
Fragments arranged by size:
301 206 184 128 32 32 32
MfeI C'AATT_G
Cuts at: 0 527 915
Size: 527 388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 741 915
Size: 555 186 174
Fragments arranged by size:
555 186 174
MslI CAynn'nnrTG
Cuts at: 0 674 915
Size: 674 241
NsiI A_TGCA'T
Cuts at: 0 800 915
Size: 800 115
PflMI CCAn_nnn'nTGG
Cuts at: 0 799 915
Size: 799 116
SacI G_AGCT'C
Cuts at: 0 213 915
Size: 213 702
SfcI C'TryA_G
Cuts at: 0 80 647 915
Size: 80 567 268
Fragments arranged by size:
567 268 80
SnaBI TAC'GTA
Cuts at: 0 165 915
Size: 165 750
SpeI A'CTAG_T
Cuts at: 0 549 855 915
Size: 549 306 60
Fragments arranged by size:
549 306 60
SspI AAT'ATT
Cuts at: 0 426 604 662 915
Size: 426 178 58 253
Fragments arranged by size:
426 253 178 58
StyI C'CwwG_G
Cuts at: 0 627 904 915
Size: 627 277 11
Fragments arranged by size:
627 277 11
TatI w'GTAC_w
Cuts at: 0 685 915
Size: 685 230
Tth111I GACn'n_nGTC
Cuts at: 0 106 767 915
Size: 106 661 148
Fragments arranged by size:
661 148 106
XmnI GAAnn'nnTTC
Cuts at: 0 425 915
Size: 425 490
Enzymes that do cut:
AccI AcuI ApoI AseI AvrII BanII BbsI BclI
BfrBI BlpI BsaAI BsaWI BseYI BsiHKAI BsmI Bsp1286I
BsrDI BstYI BstZ17I DraI DrdI EarI EcoICRI Eco57MI
EcoRI HaeII Hin4I MfeI MmeI MslI NsiI PflMI
SacI SfcI SnaBI SpeI SspI StyI TatI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BglI BglII Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII EaeI
EagI EciI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MluI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SphI SrfI StuI SwaI TaqII TstI XbaI XcmI
XhoI XmaI ZraI